Universal stress protein‐like gene from mulberry enhances abiotic stress tolerance in <i>Escherichia coli</i> and transgenic tobacco cells

Dhanyalakshmi, K. H.; Nataraja, K.

doi:10.1111/plb.13311

Cited by 16 publications

(10 citation statements)

References 45 publications

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“…Nevertheless, the gene EF1058 for ‘universal stress protein’ was found to be down‐regulated. Universal stress proteins are one of the most abundant proteins in growth‐restricted cells, thus down‐regulated genes may indicate that low pH still has a negative impact on cell growth and stress capacity to some extent (Dhanyalakshmi and Nataraja 2021). While after adding GTP, EF1058 was found to be up‐regulated.…”

Section: Resultsmentioning

confidence: 99%

RNA-seq analysis of green tea polyphenols modulation of differently expressed genes in Enterococcus faecalis under low pH

Yan

Zhou

Zheng

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: Enterococcus faecalis (E. faecalis) is a resident bacterium in the host, and can be used as probiotic additives. In this study, transcriptome technology was used to comprehensively analyse the mechanism of GTP improving the growth state of E. faecalis under low pH stress from the perspective of genes. This study showed that under low pH stress, a number of genes of E. faecalis associated with cell wall and membrane, transmembrane transport and metabolism process were significantly differentially expressed (P < 0Á05), while green tea polyphenols (GTP) intervention can partly relieve these negative influences. Significant changes were associated with selenocompound metabolism and aminoacyl-tRNA biosynthesis after the intervention of GTP (P < 0Á05). This will further deepen our understanding of the functional mechanism of GTP assisted E. faecalis in the gastrointestinal environment.

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Section: Resultsmentioning

confidence: 99%

RNA-seq analysis of green tea polyphenols modulation of differently expressed genes in Enterococcus faecalis under low pH

Yan

Zhou

Zheng

et al. 2022

Letters in Applied Microbiology

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“…Six seedlings with similar growth status were selected and divided into two groups. The AnAO5 was transiently expressed in tobacco by reference to a previous method [ 59 ]. The vector used in the experiment was pCAMBIA1305, the enzyme digestion sites were Xba I and Bam HI, and the competent cell was Agrobacterium tumefaciens GV3101.…”

Section: Methodsmentioning

confidence: 99%

Gene Profiling of the Ascorbate Oxidase Family Genes under Osmotic and Cold Stress Reveals the Role of AnAO5 in Cold Adaptation in Ammopiptanthus nanus

Zhu

Liu

et al. 2023

Plants

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The uplift of the Qinghai Tibet Plateau has led to a drastic change in the climate in Central Asia, from warm and rainy, to dry and less rainfall. Ammopiptanthus nanus, a rare evergreen broad-leaved shrub distributed in the temperate desert region of Central Asia, has survived the drastic climate change in Central Asia caused by the uplift of the Qinghai-Tibet Plateau. Ascorbate oxidase (AO) regulates the redox status of the apoplast by catalyzing the oxidation of ascorbate acid to dehydroascorbic acid, and plays a key role in the adaptation of plants to environmental changes. Analyzing the evolution, environmental response, and biological functions of the AO family of A. nanus is helpful for understanding how plant genome evolution responds to climate change in Central Asia. A total of 16 AOs were identified in A. nanus, all of which contained the ascorbate oxidase domain, most of which contained transmembrane domain, and many were predicted to be localized in the apoplast. Segmental duplication and tandem duplication are the main factors driving the gene amplification of the AO gene family in A. nanus. Gene expression analysis based on transcriptome data and fluorescence quantitative PCR, as well as enzyme activity measurements, showed that the expression levels of AO genes and total enzyme activity decreased under short-term osmotic stress and low-temperature stress, but the expression of some AO genes (AnAO5, AnAO13, and AnAO16) and total enzyme activity increased under 7 days of cold stress. AnAO5 and AnAO11 are targeted by miR4415. Further functional studies on AnAO5 showed that AnAO5 protein was localized in the apoplast. The expression of AnAO5 in yeast cells and the transient expression in tobacco enhanced the tolerance of yeast and tobacco to low-temperature stress, and the overexpression of AnAO5 enhanced the tolerance of Arabidopsis seedlings to cold stress. Our research provides important data for understanding the role of AOs in plant adaptation to environmental change.

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“…The AnTLP13 was expressed in E. coli by reference to the previous method [58]. The vector used in the experiment was pET-28a(+), the enzyme digestion sites were BamHI and HindIII, and the competent cell was E. coli BL21(DE3).…”

Section: Vector Construction and Expression In E Colimentioning

confidence: 99%

Genome-Wide Analyses of Thaumatin-like Protein Family Genes Reveal the Involvement in the Response to Low-Temperature Stress in Ammopiptanthus nanus

Liu

Sui

Wang

et al. 2023

IJMS

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Thaumatin-like proteins (TLPs), a family of proteins with high sequence similarity to thaumatin, are shown to be involved in plant defense, and are thus classified into the pathogenesis related protein family 5. Ammopiptanthus nanus is a rare evergreen broad-leaved shrub distributed in the temperate zone of Central Asia, which has a high tolerance to low-temperature stress. To characterize A. nanus TLPs and understand their roles in low-temperature response in A. nanus, a comprehensive analysis of the structure, evolution, and expression of TLP family proteins was performed. A total of 31 TLP genes were detected in the A. nanus genome, and they were divided into four groups based on their phylogenetic positions. The majority of the AnTLPs contained the conserved cysteine residues and were predicted to have the typical three-dimensional structure of plant TLPs. The primary modes of gene duplication of the AnTLP family genes were segmental duplication. The promoter regions of most AnTLP genes contain multiple cis-acting elements related to environmental stress response. Gene expression analysis based on transcriptome data and fluorescence quantitative PCR analysis revealed that several AnTLP genes were involved in cold-stress response. We further showed that a cold-induced AnTLP gene, AnTLP13, was localized in apoplast, and heterologous expression of the AnTLP13 in Escherichia coli and yeast cells and tobacco leaves enhanced low-temperature stress tolerance when compared with the control cells or seedlings. Our study provided important data for understanding the roles of TLPs in plant response to abiotic stress.

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Universal stress protein‐like gene from mulberry enhances abiotic stress tolerance in Escherichia coli and transgenic tobacco cells

Cited by 16 publications

References 45 publications

RNA-seq analysis of green tea polyphenols modulation of differently expressed genes in Enterococcus faecalis under low pH

RNA-seq analysis of green tea polyphenols modulation of differently expressed genes in Enterococcus faecalis under low pH

Gene Profiling of the Ascorbate Oxidase Family Genes under Osmotic and Cold Stress Reveals the Role of AnAO5 in Cold Adaptation in Ammopiptanthus nanus

Genome-Wide Analyses of Thaumatin-like Protein Family Genes Reveal the Involvement in the Response to Low-Temperature Stress in Ammopiptanthus nanus

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