Universal stress proteins contribute Edwardsiella piscicida adversity resistance and pathogenicity and promote blocking host immune response

Fang, Qingjian; Han, Yuexin; Shi, Yan-jie; Huang, Huiqin; Zai-guang, Fang; Hu, Yonghua

doi:10.1016/j.fsi.2019.10.035

Cited by 19 publications

(8 citation statements)

References 56 publications

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“…Bacteria were cultured in Luria-Bertani broth (LB) at 37 • C (for E. coli) or 28 • C (for E. piscicida). Where indicated, Ampicillin, Kanamycin, chloramphenicol, tetracycline, and polymyxin B, were supplemented at the concentration of 100, 50, 30, 15, and 100 µg/mL (Fang et al, 2019).…”

Section: Bacteria and Growth Conditionsmentioning

confidence: 99%

“…For high temperature resistance, TX01, TX01 yoeB, TX01 yefM-yoeB, and TX01 yefM-yoeBC were cultured in LB medium to exponential phase, then bacteria were transferred to fresh LB medium and cultured at 28 and 40 • C. Bacterial cell density was measured at different time points by determining absorbance at OD 600 as previously described (Fang et al, 2019). For oxidative stress assay, the TX01, TX01 yoeB, TX01 yefM-yoeB, and TX01 yefM-yoeBC in exponential phase were washed with PBS and resuspended in PBS.…”

Section: Resistance To Environmental Stress and To Non-immune Fish Serummentioning

confidence: 99%

“…The fish were maintained at 25 ± 1 • C in aerated water and fed daily with commercial dry pellets. Fish were acclimatized in the laboratory for 2 weeks before experimental manipulation (Fang et al, 2019). To make sure that the fish were not infected by bacteria, randomly selected fish were tested for bacteria in the tissues, including blood, liver, kidney, and spleen.…”

Section: Fish and Experimental Challenges For Bacterial Disseminationmentioning

confidence: 99%

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Edwardsiella piscicida YefM-YoeB: A Type II Toxin-Antitoxin System That Is Related to Antibiotic Resistance, Biofilm Formation, Serum Survival, and Host Infection

DongMei

Shi

et al. 2021

Front. Microbiol.

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The emergence of drug resistant bacteria is a tricky and confronted problem in modern medicine, and one of important reasons is the widespread of toxin-antitoxin (TA) systems in pathogenic bacteria. Edwardsiella piscicida (also known as E. tarda) is the leading pathogen threatening worldwide fresh and seawater aquaculture industries and has been considered as a model organism for studying intracellular and systemic infections. However, the role of type II TA systems are completely unknown in aquatic pathogenic bacteria. In this study, we identified and characterized a type II TA system, YefM-YoeB, of E. piscicida, where YefM is the antitoxin and YoeB is the toxin. yefM and yoeB are co-expressed in a bicistronic operon. When expressed in E. coli, YoeB cause bacterial growth arrest, which was restored by the addition of YefM. To investigate the biological role of the TA system, two markerless yoeB and yefM-yoeB in-frame mutant strains, TX01ΔyoeB and TX01ΔyefM-yoeB, were constructed, respectively. Compared to the wild strain TX01, TX01ΔyefM-yoeB exhibited markedly reduced resistance against oxidative stress and antibiotic, and markedly reduced ability to form persistent bacteria. The deletion of yefM-yoeB enhanced the bacterial ability of high temperature tolerance, biofilm formation, and host serum resistance, which is the first study about the relationship between type II TA system and serum resistance. In vitro infection experiment showed that the inactivation of yefM-yoeB greatly enhanced bacterial capability of adhesion in host cells. Consistently, in vivo experiment suggested that the yefM-yoeB mutation had an obvious positive effect on bacteria dissemination of fish tissues and general virulence. Introduction of a trans-expressed yefM-yoeB restored the virulence of TX01ΔyefM-yoeB. These findings suggest that YefM-YoeB is involved in responding adverse circumstance and pathogenicity of E. piscicida. In addition, we found that YefM-YoeB negatively autoregulated the expression of yefM-yoeB and YefM could directly bind with own promoter. This study provides first insights into the biological activity of type II TA system YefM-YoeB in aquatic pathogenic bacteria and contributes to understand the pathogenesis of E. piscicida.

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Section: Bacteria and Growth Conditionsmentioning

confidence: 99%

Section: Resistance To Environmental Stress and To Non-immune Fish Serummentioning

confidence: 99%

Section: Fish and Experimental Challenges For Bacterial Disseminationmentioning

confidence: 99%

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Edwardsiella piscicida YefM-YoeB: A Type II Toxin-Antitoxin System That Is Related to Antibiotic Resistance, Biofilm Formation, Serum Survival, and Host Infection

DongMei

Shi

et al. 2021

Front. Microbiol.

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“…As a member of Enterobacteriales, E. tarda can survive and replicate in an acidic environment, but the underlying mechanisms remain unknown. Although some genes or factors, such as Usp13, TAM, HutZ, TrxH, RpoN, and RpoS have been reported to participate in acid tolerance [ 37 , 47 – 50 ], there is no report about the function of traditional or classical acid resistance in E. tarda . In this study, our results show that compared to wild type TX01, the growth of three mutants TX01Δ cadA , TX01Δ cadB , and TX01Δ cadBA appeared to be retarded obviously when exposed to an acidic environment (pH = 5.5 to 4.5), especially cadA delete mutant TX01Δ cadA .…”

Section: Discussionmentioning

confidence: 99%

“…These results illustrate that cadBA operon negatively participated in the biofilm formation. Different from cadBA , hutZ mutation and usp13 mutation in E. tarda decreased both bacterial resistance against acid stress and biofilm formation [ 37 , 47 ]. YefM-YoeB operon, a type II TA system, is not related to acid tolerance, but its mutation enhanced biofilm formation of E. tarda [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Acid resistance system CadBA is implicated in acid tolerance and biofilm formation and is identified as a new virulence factor of Edwardsiella tarda

Huo

et al. 2021

Vet Res

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Edwardsiella tarda is a facultative intracellular pathogen in humans and animals. The Gram-negative bacterium is widely considered a potentially important bacterial pathogen. Adaptation to acid stress is important for the transmission of intestinal microbes, so the acid-resistance (AR) system is essential. However, the AR systems of E. tarda are totally unknown. In this study, a lysine-dependent acid resistance (LDAR) system in E. tarda, CadBA, was characterized and identified. CadB is a membrane protein and shares high homology with the lysine/cadaverine antiporter. CadA contains a PLP-binding core domain and a pyridoxal phosphate-binding motif. It shares high homology with lysine decarboxylase. cadB and cadA are co-transcribed under one operon. To study the function of the cadBA operon, isogenic cadA, cadB and cadBA deletion mutant strains TX01ΔcadA, TX01ΔcadB and TX01ΔcadBA were constructed. When cultured under normal conditions, the wild type strain and three mutants exhibited the same growth performance. However, when cultured under acid conditions, the growth of three mutants, especially TX01ΔcadA, were obviously retarded, compared to the wild strain TX01, which indicates the important involvement of the cadBA operon in acid resistance. The deletion of cadB or cadA, especially cadBA, significantly attenuated bacterial activity of lysine decarboxylase, suggesting the vital participation of cadBA operon in lysine metabolism, which is closely related to acid resistance. The mutations of cadBA operon enhanced bacterial biofilm formation, especially under acid conditions. The deletions of the cadBA operon reduced bacterial adhesion and invasion to Hela cells. Consistently, the deficiency of cadBA operon abated bacterial survival and replication in macrophages, and decreased bacterial dissemination in fish tissues. Our results also show that the expression of cadBA operon and regulator cadC were up-regulated upon acid stress, and CadC rigorously regulated the expression of cadBA operon, especially under acid conditions. These findings demonstrate that the AR CadBA system was a requisite for the resistance of E. tarda against acid stress, and played a critical role in bacterial infection of host cells and in host tissues. This is the first study about the acid resistance system of E. tarda and provides new insights into the acid-resistance mechanism and pathogenesis of E. tarda.

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Exogenous indole modulates several CpxRA-mediated virulence-related parameters of Edwardsiella piscicida in vitro

Gou,

Fang,

et al. 2024

Mar Life Sci Technol

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Universal stress proteins contribute Edwardsiella piscicida adversity resistance and pathogenicity and promote blocking host immune response

Cited by 19 publications

References 56 publications

Edwardsiella piscicida YefM-YoeB: A Type II Toxin-Antitoxin System That Is Related to Antibiotic Resistance, Biofilm Formation, Serum Survival, and Host Infection

Edwardsiella piscicida YefM-YoeB: A Type II Toxin-Antitoxin System That Is Related to Antibiotic Resistance, Biofilm Formation, Serum Survival, and Host Infection

Acid resistance system CadBA is implicated in acid tolerance and biofilm formation and is identified as a new virulence factor of Edwardsiella tarda

Exogenous indole modulates several CpxRA-mediated virulence-related parameters of Edwardsiella piscicida in vitro

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