Unraveling response mechanism of photosynthetic metabolism and respiratory metabolism to uranium-exposure in Vicia faba

Zhang, Yu; Lai, Jin-long; Ji, Xiaohui; Luo, Xin

doi:10.1016/j.jhazmat.2020.122997

Cited by 41 publications

(12 citation statements)

References 48 publications

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“…The fresh weight, length, outer diameter, and wall thickness of each bamboo internode were determined. Leaf photosynthetic pigments were extracted with ethanol-acetone solution and quantified spectrophotometrically ( Zhang Y. et al, 2020 ). Leaf nitrogen content was determined using a Chlorophyll Analyzer (TYS-4 N model, Beijing Zhongke Weihe Technology Development Co., Ltd. China).…”

Section: Methodsmentioning

confidence: 99%

“…The soil metabolite data matrix was compared against KEGG 1 datasets to determine the KEGG IDs of the metabolites; the metabolic pathways associated with the DEM were filtered according to the annotated results. The screening threshold for metabolic pathway enrichment analysis of DEMs was p < 0.05 ( Zhang Y. et al, 2020 ). Based on the metabolite data matrix, galactose, nucleotide, and amino acid metabolism under different fertilization conditions were then evaluated.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome

Wang²,

Xu³

et al. 2023

Front. Microbiol.

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Organic and microbial fertilizers have potential advantages over inorganic fertilizers in improving soil fertility and crop yield without harmful side-effects. However, the effects of these bio-organic fertilizers on the soil microbiome and metabolome remain largely unknown, especially in the context of bamboo cultivation. In this study, we cultivated Dendrocalamus farinosus (D. farinosus) plants under five different fertilization conditions: organic fertilizer (OF), Bacillus amyloliquefaciens bio-fertilizer (Ba), Bacillus mucilaginosus Krassilnikov bio-fertilizer (BmK), organic fertilizer plus Bacillus amyloliquefaciens bio-fertilizer (OFBa), and organic fertilizer plus Bacillus mucilaginosus Krassilnikov bio-fertilizer (OFBmK). We conducted 16S rRNA sequencing and liquid chromatography/mass spectrometry (LC–MS) to evaluate the soil bacterial composition and soil metabolic activity in the different treatment groups. The results demonstrate that all the fertilization conditions altered the soil bacterial community composition. Moreover, the combination of organic and microbial fertilizers (i.e., in the OFBa and OFBmK groups) significantly affected the relative abundance of soil bacterial species; the largest number of dominant microbial communities were found in the OFBa group, which were strongly correlated with each other. Additionally, non-targeted metabolomics revealed that the levels of soil lipids and lipid-like molecules, and organic acids and their derivatives, were greatly altered under all treatment conditions. The levels of galactitol, guanine, and deoxycytidine were also markedly decreased in the OFBa and OFBmK groups. Moreover, we constructed a regulatory network to delineated the relationships between bamboo phenotype, soil enzymatic activity, soil differential metabolites, and dominant microbial. The network revealed that bio-organic fertilizers promoted bamboo growth by modifying the soil microbiome and metabolome. Accordingly, we concluded that the use of organic fertilizers, microbial fertilizers, or their combination regulated bacterial composition and soil metabolic processes. These findings provide new insights into how D. farinosus-bacterial interactions are affected by different fertilization regiments, which are directly applicable to the agricultural cultivation of bamboo.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome

Wang²,

Xu³

et al. 2023

Front. Microbiol.

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Section: -Introductionmentioning

confidence: 99%

“…During the last decade, several comparative genomic approaches and/or quantitative analyses performed in A. thaliana , Vicia faba and Ipomoea batatas roots have provided insight into global gene expression and metabolic adjustments in response to U (Doustaly et al, 2014; Lai et al, 2020; Sarthou et al, 2020; Zhang et al, 2020; Lai et al, 2021, Vallet et al, 2023; Xiao et al, 2023; Chen et al, 2023). To shed light on the consequences of uranyl ions on the soluble proteome of Arabidopsis root and shoot cells, we previously developed an ionomic and top-down proteomic analysis coupled with biochemical and structural approaches (Sarthou et al, 2020; Vallet et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of the membrane- and cell wall-associated proteome ofArabidopsis thalianaroots in response to uranium stress

Przybyla-Toscano,

Chetouhi,

Balliau

et al. 2024

Preprint

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Uranium (U) is a non-essential and toxic metal for plants, which have the ability to take up uranyl ions from the soil and preferentially accumulate them in the roots. We showed that the bulk of the radionuclide accumulates in the root insoluble proteome of Arabidopsis plants challenged with U. Therefore, to elucidate new molecular mechanisms related to U stress response and tolerance, we used label-free quantitative proteomics to analyze the dynamics of the root membrane- and cell wall-enriched proteome under U stress. Of the 2,802 proteins identified, 458 showed differential accumulation in response to U. Biological processes affected by U include response to stress, amino acid metabolism, and previously unexplored functions associated with membranes and the cell wall. Indeed, our analysis supports a dynamic and complex reorganization of the cell wall in response to U stress, including lignin and suberin synthesis, pectin modifications, polysaccharide hydrolysis, and Casparian strips formation. Water flux through aquaporins and vesicular trafficking were also significantly perturbed by U stress. Finally, the abundance of metal transporters and iron, calcium, and other metal-binding proteins was affected by U. These proteins may play a role in controlling the fate and toxicity of U in plants.

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“…There are a few studies pertaining to the interactions of RNs with plants at the molecular level. These reports include investigations of plant cell cultures and whole plants as well as modeling studies of RN transport within the plant. ,,,− However, most studies in this field focus on determining transfer factors. Consequently, there is currently insufficient information about the uptake processes taking place …”

Section: Introductionmentioning

confidence: 99%

Bioassociation of U(VI) and Eu(III) by Plant (Brassica napus) Suspension Cell Cultures—A Spectroscopic Investigation

Jessat

Sachs

Moll

et al. 2021

Environ. Sci. Technol.

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In this study, we investigated the interaction of U(VI) and Eu(III) with Brassica napus suspension plant cells as a model system. Concentration-dependent (0–200 μM) bioassociation experiments showed that more than 75% of U(VI) and Eu(III) were immobilized by the cells. In addition to this phenomenon, time-dependent studies for 1 to 72 h of exposure showed a multistage bioassociation process for cells that were exposed to 200 μM U(VI), where, after initial immobilization of U(VI) within 1 h of exposure, it was released back into the culture medium starting within 24 h. A remobilization to this extent has not been previously observed. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to correlate the bioassociation behavior of Eu and U with the cell vitality. Speciation studies by spectroscopy and in silico methods highlighted various U and Eu species over the course of exposure. We were able to observe a new U species, which emerged simultaneously with the remobilization of U back into the solution, which we assume to be a U(VI) phosphate species. Thus, the interaction of U(VI) and Eu(III) with released plant metabolites could be concluded.

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Unraveling response mechanism of photosynthetic metabolism and respiratory metabolism to uranium-exposure in Vicia faba

Cited by 41 publications

References 48 publications

Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome

Bio-organic fertilizers improve Dendrocalamus farinosus growth by remolding the soil microbiome and metabolome

Dynamics of the membrane- and cell wall-associated proteome ofArabidopsis thalianaroots in response to uranium stress

Bioassociation of U(VI) and Eu(III) by Plant (Brassica napus) Suspension Cell Cultures—A Spectroscopic Investigation

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