Untangling the biological effects of cerium oxide nanoparticles: the role of surface valence states

Pulido-Reyes, Gerardo; Rodea-Palomares, Ismael; Das, Soumen; Sakthivel, T.; Leganés, Francisco; Rosal, Roberto; Seal, Sudipta; Fernández-Piñas, Francisca

doi:10.1038/srep15613

Cited by 257 publications

(183 citation statements)

References 49 publications

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“…In such hypothesis, photosynthetic cells would be more affected by the reduction of cerium (IV) to cerium (III) (Rodea-Palomares et al, 2012). In agreement with this, the main driver of toxicity was found to be the percentage of surface content of Ce 3+ sites (Pulido-Reyes et al, 2015).…”

Section: Discussionsupporting

confidence: 69%

Nanoceria and bulk cerium oxide effects on the germination of asplenium adiantum-nigrum spores

et al. 2016

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Aim of the study: The effect of cerium oxide engineered nanoparticles on the spore germination of the fern Asplenium adiantumnigrum.Area of study: France, Britanny Region, Finistére Department, Plougonvelin, in rocks near the sea. Material and methods: Asplenium spores were cultured in vitro on agar medium with Nano-CeO 2 (less than 25 nm particle size) and bulk-CeO 2 . The addition of each nano-and bulk particles ranged from 0 to 3000 mg L -1 . Observations on rhizoidal and prothallial cells during first stages of gametophyte development were made. The No-Observed-Adverse-Effect concentration (NOAEC) and Lowest-Observed-Adverse-Effect-Concentration (LOEC) values for spore germination rate data were analyzed.Main results: Germination was speeded up by 100 to 2000 mg L -1 nanoceria, while bulk cerium oxide had the same effect for 500 to 2000 mg L -1 concentrations. Present results showed cellular damage in the protonema while rhizoid cells seemed not to be affected, as growth and membrane integrity remained.Research highlights: Both nanosized and bulk cerium oxide are toxic for the fern Asplenium adiantum-nigrum, although diverse toxicity patterns were shown for both materials. Diverse toxic effects have been observed: chloroplast membrane damage and lysis, cell wall and membrane disruption which leads to cell lysis; and alterations in morphology and development.

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Section: Discussionsupporting

confidence: 69%

Nanoceria and bulk cerium oxide effects on the germination of asplenium adiantum-nigrum spores

et al. 2016

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“…Moreover, the most antibacterial effects due to the highest percentage of surface Ce 3+ of NP are in agreement with Pulido-Reyes et al's observations. 67 …”

Section: Biological Applications Of Ceo 2 -Nps Antibacterial Effectmentioning

confidence: 99%

“…, there seemed to be a shift from SOD activity to catalase mimetic activity; however, the mechanisms and whether the observed biological effect reported at their study may also occur in other cellular systems, requires further investigation. 67 However, there is no report on the effect of applying green methods of CeO 2 -NPs synthesis on the percentage of surface Ce 3+ of NPs and this should be investigated to clearly demonstrate the effect of green synthesis of CeO 2 -NPs on their cytotoxicity.…”

Section: Morphologymentioning

confidence: 99%

Cerium oxide nanoparticles: green synthesis and biological applications

Charbgoo

Ahmad

Darroudi

2017

IJN

264

125

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CeO 2 nanoparticles (NPs) have shown promising approaches as therapeutic agents in biology and medical sciences. The physicochemical properties of CeO 2 -NPs, such as size, agglomeration status in liquid, and surface charge, play important roles in the ultimate interactions of the NP with target cells. Recently, CeO 2 -NPs have been synthesized through several bio-directed methods applying natural and organic matrices as stabilizing agents in order to prepare biocompatible CeO 2 -NPs, thereby solving the challenges regarding safety, and providing the appropriate situation for their effective use in biomedicine. This review discusses the different green strategies for CeO 2 -NPs synthesis, their advantages and challenges that are to be overcome. In addition, this review focuses on recent progress in the potential application of CeO 2 -NPs in biological and medical fields. Exploiting biocompatible CeO 2 -NPs may improve outcomes profoundly with the promise of effective neurodegenerative therapy and multiple applications in nanobiotechnology.

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“…27,28 UV–Vis absorbance is a facile method to determine the oxidation state of CNPs in aqueous solution. 50 None of the CNPs demonstrated significant peak at 250 nm (Fig. 3b), suggesting low or negligible percentage of Ce 3+ population.…”

Section: Resultsmentioning

confidence: 92%

“…3b), suggesting low or negligible percentage of Ce 3+ population. 50 SR, LR and sphere CNPs showed strong absorbance peaks at 300 nm (Fig. 3b), suggesting predominant presence of Ce 4+ population.…”

Section: Resultsmentioning

confidence: 97%

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

et al. 2017

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Introduction Lack of effective pharmacological treatment makes valvular calcification a significant clinical problem in patients with valvular disease and bioprosthetic/mechanical valve replacement therapies. Elevated levels of reactive oxygen species (ROS) in valve tissue have been identified as a prominent hallmark and driving factor for valvular calcification. However, the therapeutic value of ROS-modulating agents for valvular calcification remains elusive. We hypothesized that ROS-modulating shape-specific cerium oxide nanoparticles (CNPs) will inhibit oxidative stress-induced valvular calcification. CNPs are a class of self-regenerative ROS-modulating agents, which can switch between Ce3+ and Ce4+ in response to oxidative microen-vironment. In this work, we developed oxidative stress-induced valve calcification model using two patient-derived stenotic valve interstitial cells (hVICs) and investigated the therapeutic effect of shape-specific CNPs to inhibit hVIC calcification. Methods Human valvular interstitial cells (hVICs) were obtained from a normal healthy donor and two patients with calcified aortic valves. hVICs were characterized for their phenotypic (mesenchymal, myofibroblast and osteoblast) marker expression by qRT-PCR and antioxidant enzymes activity before and after exposure to hydrogen peroxide (H2O2)-induced oxidative stress. Four shape-specific CNPs (sphere, short rod, long rod, and cube) were synthesized via hydrothermal or ultra-sonication method and characterized for their biocompatibility in hVICs by alamarBlue® assay, and ROS scavenging ability by DCFH-DA assay. H2O2 and inorganic phosphate (Pi) were co-administrated to induce hVIC calcification in vitro as demonstrated by Alizarin Red S staining and calcium quantification. The effect of CNPs on inhibiting H2O2-induced hVIC calcification was evaluated. Results hVICs isolated from calcified valves exhibited elevated osteoblast marker expression and decreased antioxidant enzyme activities compared to the normal hVICs. Due to the impaired antioxidant enzyme activities, acute H2O2-induced oxidative stress resulted in higher ROS levels and osteoblast marker expression in both diseased hVICs when compared to the normal hVICs. Shape-specific CNPs exhibited shape-dependent abiotic ROS scavenging ability, and excellent cytocompatibility. Rod and sphere CNPs scavenged H2O2-induced oxidative stress in hVICs in a shape- and dose-dependent manner by lowering intracellular ROS levels and osteoblast marker expression. Further, CNPs also enhanced activity of antioxidant enzymes in hVICs to combat oxidative stress. Cube CNPs were not effective ROS scavengers. The addition of H2O2 in the Pi-induced calcification model further increased calcium deposition in vitro in a time-dependent manner. Co-administration of rod CNPs with Pi and H2O2 mitigated calcification in the diseased hVICs. Conclusions We demonstrated that hVICs derived from calcified valves exhibited impaired antioxidant defense mechanisms and were more susceptible to oxidative stress th...

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Untangling the biological effects of cerium oxide nanoparticles: the role of surface valence states

Cited by 257 publications

References 49 publications

Nanoceria and bulk cerium oxide effects on the germination of asplenium adiantum-nigrum spores

Nanoceria and bulk cerium oxide effects on the germination of asplenium adiantum-nigrum spores

Cerium oxide nanoparticles: green synthesis and biological applications

Shape-Specific Nanoceria Mitigate Oxidative Stress-Induced Calcification in Primary Human Valvular Interstitial Cell Culture

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