Untersuchungen zum katabolen Mucopolysaccharid-Protein-Stoffwechsel in menschlichen Organen

Platt, D.; Stein, U.

doi:10.1515/cclm.1969.7.4.374

Cited by 14 publications

(5 citation statements)

References 8 publications

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“…Refer to P latt [11a] and Platt and Stein [12] for the measurement of the protein and enzyme activity.…”

Section: Methodsmentioning

confidence: 99%

An Age-Dependent Determination of Lysosomal Enzyme Activities, as well as the Measurements of the Incorporation of 14-C-Proline and 14-C-GIucosamine in a Subcutaneously Implanted Polyether Sponge

Platt¹,

Rühl²

1972

Gerontology

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Synthetic sponge (polyether) was implanted in the subcutis of male albino rats of 3 age groups: 6 weeks old, 9 months old and 20–22 months old. Morphological and biological examinations were made on the granulation tissue which migrated into it. As parameters for the inter-cellular catabolic process, the activity measurements of the β-glucuronidase, β-acetylglucosaminidase, cathepsin D and a collagen catabolic enzyme were conducted. The incorporation rate of ¹⁴C-glucosamine and ¹⁴C-ρroline served as an indicator for the synthesis mechanisms. In addition to this, the DNA content of the sponge was determined. The measurements of all the above-named parameters were made 14, 21 and 30 days after the implantation, on the sponge implanted in the paravertebral subcutis. The results demonstrate that, depending on the duration of implantation as well as on the age of the animal, distinct alterations of the examined parameters take place. A correlation of the various parameters to one another could not be established. The findings were discussed in relation to those found in the literature.

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“…Refer to P latt [11a] and Platt and Stein [12] for the measurement of the protein and enzyme activity.…”

Section: Methodsmentioning

confidence: 99%

An Age-Dependent Determination of Lysosomal Enzyme Activities, as well as the Measurements of the Incorporation of 14-C-Proline and 14-C-GIucosamine in a Subcutaneously Implanted Polyether Sponge

Platt¹,

Rühl²

1972

Gerontology

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“…Derzeit sind keine biosynthetischen Enzyme (z.B. Xylosyltransferasen, Sulfotransferasen) bekannt, deren Bestimmung im Se-N-Acetyl-ft-g/ucosamtpidase ($-Hex) Die Aktivität der ß-Hex im Serum ist signifikant bei chronischer Hepatitis und in der Frühphase der Leberzirrhose erhöht, während terminale Stadien nur geringe Aktivitätszunahmen zeigen (66)(67)(68)(69). Die Höhe der Serum-und Leberaktivität korreliert mit dem histologisch beurteilten Aktivitätsgrad der bindegewebigen Umgestaltung der Leber (66,70,71) und erlaubt somit eine Abgrenzung der mit Normalaktivitäten einhergehenden chronisch-persistierenden von den chronischaggressiven Hepatitiden und Zirrhosen.…”

Section: Parameter Des Proteoglykanstoffwechselsunclassified

“…Die Höhe der Serum-und Leberaktivität korreliert mit dem histologisch beurteilten Aktivitätsgrad der bindegewebigen Umgestaltung der Leber (66,70,71) und erlaubt somit eine Abgrenzung der mit Normalaktivitäten einhergehenden chronisch-persistierenden von den chronischaggressiven Hepatitiden und Zirrhosen. Da jedoch auch akute Hepatitiden (66,69,72) und Hepatome (66,68) sowie einige nicht-hepatische Erkrankungen zu stark erhöhten ß-Hex-Aktivitäten führen, bleibt die Spezifität dieses Parameters per se für fibrosierende Lebererkrankungen eingeschränkt. Isoenzymbestimmungen (71,73) bringen möglicherweise eine Steigerung der diagnostischen Präzision.…”

Section: Parameter Des Proteoglykanstoffwechselsunclassified

“…Bei tierexperimentellen (68) und menschlichen (68,69,72) Lebererkrankungen sind die Serum-und Leberaktivitäten der beiden Enzyme eleviert. Die höchsten Aktivitäten finden sich bei akuter Hepatitis (69,72,73).…”

Section: Hyaluronidase Und $-D-glucuronidaseunclassified

“…Die höchsten Aktivitäten finden sich bei akuter Hepatitis (69,72,73). In der diagnostischen Wertigkeit für Leberfibrosen sind die beiden .Enzyme der ß-Hex unterlegen (66), in der Durchführbarkeit ihrer Testmethoden bieten sie ebenfalls keine Vorteile (Tab.6).…”

Section: Hyaluronidase Und $-D-glucuronidaseunclassified

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Übersichtsreferate

1979

LaboratoriumsMedizin

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Biochemical indicators of the degree or activity of the fibrotic transformation of the chronically injured liver (parameters of fibrosis) were summarized and their diagnostic usefulness on the basis of the pathobiochemical distortion of hepatic connective tissue metabolism critically evaluated. Specificity, sensitivity and practicability of the tests of the majority of the presently available parameters of liver fibrosis are unsatisfactory. Comparatively the best diagnostic informations are obtained by the determinations of the activities of collagen-protyl-hydroxylase in liver biopsy specimens and collagen peptidase r monoamine oxidase or N-acetyl· -D -glucosaminidase in serum. However, the disease -(fibrosis-) related and organ-(liver-)related specificity of these serum parameters also is low in part

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Mucopolysaccharidosis types I, II, IIIA and V

Dekaban

Constantopoulos

1977

Acta Neuropathol

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Histochemical and electron microscopic studies of the brains inclusive of the leptomeninges containing large blood vessels from 7 patients with mucopolysaccharidosis (MPS) I, II, IIIA and V showed marked increase in mesenchymal elements and the generalized presence of characteristic lesions around cerebral veins and arteries. The periadventitial space was greatly distended and filled with viscous fluid and numerous mononuclear cells containing large cytoplasmic vacuoles; these cells stained positively for glycosaminoglycans (GAG). In contrast, the neurons showed only a slight increase of GAG over the normal controls but contained an excessive amount of glycolipid-like material. The amount of GAG in the leptomeninges, inclusive of the large blood vessels, was 10.8, 6.5, 4.5 and 2.2 times greater in patients with MPS I, II, V and IIIA respectively, than the mean of unaffected controls. Dermatan sulfate (DS) accounted for most of the GAG increase in MPS I, II and V [mixed excretors of DS and heparan sulfate (HS)], and HS for the GAG increase in MPS IIIA (HS excretor). With the exception of the patient with MPS IIIA, whose GAG content and composition were the same in both the neural and mesenchymal elements, in all the other MPS types the mesenchymal elements contained more GAG, with a preponderance of DS. We conclude that the mesenchymal elements contribute substantially to the increased content of GAG in the brain and its coverings, mostly in the form of dermatan sulfate.

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Untersuchungen zum katabolen Mucopolysaccharid-Protein-Stoffwechsel in menschlichen Organen

Cited by 14 publications

References 8 publications

An Age-Dependent Determination of Lysosomal Enzyme Activities, as well as the Measurements of the Incorporation of 14-C-Proline and 14-C-GIucosamine in a Subcutaneously Implanted Polyether Sponge

An Age-Dependent Determination of Lysosomal Enzyme Activities, as well as the Measurements of the Incorporation of 14-C-Proline and 14-C-GIucosamine in a Subcutaneously Implanted Polyether Sponge

Übersichtsreferate

Mucopolysaccharidosis types I, II, IIIA and V

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