UPLC-ESI-MS/MS-Based Widely Targeted Metabolomics Analysis of Wood Metabolites in Teak (Tectona grandis)

Abstract

The properties of teak wood, such as natural durability and beautiful color, are closely associated with wood extractives. In order to further understand the performance differences between teak heartwood and sapwood, we analyzed the chemical components of extractives from 12 wood samples using an ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS)-based metabolomics approach. In total, 691 metabolites were identified, and these were classified into 17 different categories. Clustering analysis and principal component analysis of metabolites showed that heartwood samples could be clearly separated from sapwood samples. Differential metabolite analysis revealed that the levels of primary metabolites, including carbohydrates, amino acids, lipids, and nucleotides, were significantly lower in the heartwood than in the sapwood. Conversely, many secondary metabolites, including flavonoids, phenylpropanoids, and quinones, had higher levels in the heartwood than in the sapwood. In addition, we detected 16 specifically expressed secondary metabolites in the heartwood, the presence of which may correlate with the durability and color of teak heartwood. Our study improves the understanding of differential metabolites between sapwood and heartwood of teak, and provides a reference for the study of heartwood formation.

Integrated Data Reveal The Flavonoid Biosynthesis Metabolic Pathway In Semen Ziziphi Spinosae Using Transcriptomic And Metabolomic

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Analysis of metabolites in young and mature Docynia delavayi (Franch.) Schneid leaves using UPLC-ESI-MS/MS

“…Of the three sequencing technologies in metabolomics, widely targeted metabolomics integrates the advantages of both targeted and non-targeted metabolomics ( Sun, 2019 ). For the past few years, UPLC-ESI-MS ( Chen et al, 2013 ) has been widely used as a popular technique for the analysis and identification of plant metabolites because it saves time, uses less solvent, and provides an accurate compound determination ( Li et al, 2019 ; Wang et al, 2019a ; Yang et al, 2020 ; Zhu, Yin & Yang, 2020 ; Zou et al, 2020 ).…”

Soil Bacterial Community Shifts Are Driven by Soil Nutrient Availability along a Teak Plantation Chronosequence in Tropical Forests in China

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