UPLC-MS fingerprints, phytochemicals and quality evaluation of flavonoids from Abrus precatorius leaves

He, Cuimin; Huang, Weibin; Xue, Xianmei; Liang, Zhike; Hao, Yue; Li, Kunping; Yuan, Xiaohong

doi:10.1016/j.jfca.2022.104585

Cited by 13 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The area where the A. precatorius plant habitat is the main factor influencing the decrease in the flavonoid content in A. precatorius leaves. In addition, the active components in A. precatorius leaves that grow for more than one year are not positively related to the year of plant growth, which may be related to the catalysis and decomposition of photosyntheticrelated enzymes (He et al, 2022).…”

Section: Disc Testmentioning

confidence: 92%

Potential Use of Saga Leaf Extract (Abrus precatorius) as Anti Bacteria Aeromonas hydrophila by in Vitro

Kenitasari,

Prajitno,

Wilujeng

et al. 2023

jppipa, pendidikan ipa, fisika, biologi, kimia

View full text Add to dashboard Cite

Many diseases are caused by bacterial pathogens, for example, Aeromonas hydrophila. One alternative that can be done to overcome this problem is to use saga leaves (Abrus precatorius) which have bioactive ingredients as antibacterial ingredients. The aims of this study were to determine the antibacterial activity of saga (A. precatorius) leaf extract, to study the character and structure of bacteria inhibited by saga (A. precatorius) leaf extract and to study the bioactive components contained in saga (A. precatorius) leaf extract. The results of the disc test showed that the average inhibition zone was 14,29 mm while the MIC test results showed that at the dose 1 mg/L capable of inhibiting bacterial growth (bacteriostatic) and dose 10 mg/L It has been able to kill bacteria (bactericidal). Crude extract of saga leaf extract (A. precatorius) affects the character and structure of A. hydrophila bacteria by damaging the cell walls and cell membranes of bacteria. The results of Infrared Spectrophotometry analysis of saga (A. precatorius) leaf extract and Ultra Violet Spectrophotometry analysis with 96% absorption ethanol were suspected to contain flavonoid compounds

show abstract

Section: Disc Testmentioning

confidence: 92%

Potential Use of Saga Leaf Extract (Abrus precatorius) as Anti Bacteria Aeromonas hydrophila by in Vitro

Kenitasari,

Prajitno,

Wilujeng

et al. 2023

jppipa, pendidikan ipa, fisika, biologi, kimia

View full text Add to dashboard Cite

show abstract

“…Moreover, only using the retention time of the reference substance to identify the chromatographic peak is prone to cause false-positive results, with low specificity and accuracy. LC-MS has been proven to be a powerful technique for the analysis and identification of natural compounds [20] and has drawn much attention in phytochemical analysis [21][22][23][24]. UPLC holds advantages over conventional HPLC with increased operation speed and improved sensitivity [25].…”

Section: Introductionmentioning

confidence: 99%

Quality Evaluation of Peony Petals Based on the Chromatographic Fingerprints and Simultaneous Determination of Sixteen Bioactive Constituents Using UPLC-DAD-MS/MS

Li,

Ma,

et al. 2023

Molecules

View full text Add to dashboard Cite

In this study, a validated quality evaluation method with peony flower fingerprint chromatogram combined with simultaneous determination of sixteen bioactive constituents was established using UPLC-DAD-MS/MS. The results demonstrated that the method was stable, reliable, and accurate. The UPLC chemical fingerprints of 12 different varieties of peonies were established and comprehensively evaluated by similarity evaluation (SE), hierarchical cluster analysis (HCA), principal component analysis (PCA), and quantification analysis. The results of SE indicated that similar chemical components were present in these samples regardless of variety, but there were significant differences in the content of chemical components and material basis characteristics. The results of HCA and PCA showed that 12 varieties of samples were divided into two groups. Four flavonoids (11, 12, 13, and 16), five monoterpenes and their glycosides (3, 4, 6, 14, and 15), three tannins (7, 9, and 10), three phenolic acids (1, 2, and 5), and one aromatic acid (8) were identified from sixteen common peaks by standards and liquid chromatography–mass spectrometry (LC–MS). The simultaneous quantification of six types of components was conducted with the 12 samples, it was found that the sum contents of analytes varied obviously for peony flower samples from different varieties. The content of flavonoids, tannins, and monoterpenes (≥19.34 mg/g) was the highest, accounting for more than 78.45% of the total compounds. The results showed that the flavonoids, tannins, and monoterpenes were considered to be the key indexes in the classification and quality assessment of peony flower. The UPLC-DAD-MS/MS method coupled with multiple compounds determination and fingerprint analysis can be effectively applied as a feature distinguishing method to evaluate the compounds in peony flower raw material for product quality assurance in the food, pharmaceutical, and cosmetic industries. Moreover, this study provides ideas for future research and the improvement of products by these industries.

show abstract

“…DNA markers are an effective method for analyzing phylogenetic relationships and evolutionary processes and the most commonly used DNA markers include ITS, matK, rpoB, rbcL, psbA-trnH, and others (Adolfo et al 2022; Kurian et al 2020;Li et al 2022). As single DNA markers cannot distinguish genetic differences in closely related species, combined sequences (such as ITS + trnL-trnF (Feng et al 2019), rbcL + matK + ITS2 (Valuyskikh et al 2020) have been extensively used.…”

Section: Introductionmentioning

confidence: 99%

“…The content of leonurine and stachydrine was generally determined by highperformance liquid chromatography (HPLC). HPLC is characterized by fast analysis speed, high sensitivity, good separation e ciency, and low sample usage (Barzdina et al 2022;He et al 2022;. It has been widely used in the ngerprinting of medicinal plants, content determination of the active ingredients, and species identi cation.…”

Section: Introductionmentioning

confidence: 99%

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

Han

Wen

Chen

et al. 2023

Genet Resour Crop Evol

View full text Add to dashboard Cite

Leonurus japonicus Houtt. (Labiatae), a perennial herb, is used to treat cardiovascular, uterine, and gynecological diseases. In the present study, a phylogenetic tree was constructed based on the ITS + psbA-trnH + rbcL + rpoB concatenation sequence, and partial least squares-discriminant analysis (PLS-DA) was performed based on high-performance liquid chromatography. The phylogenetic tree and PLS-DA were combined to correlate genetic and chemical differences among L. japonicus derived from different origins. The results showed that the concatenation sequence could distinguish among L. japonicus from different origins. Moreover, chemical analysis revealed intergroup differences, but the results were not of su ciently high quality as that of molecular phylogeny. Furthermore, the results of combined chemical and phylogenetic analyses suggested that differences in metabolites are in uenced by not only genetic differences but also environmental factors. These results provide valuable information for the arti cial cultivation of L. japonicus and new ideas for improving its quality.

show abstract

UPLC-MS fingerprints, phytochemicals and quality evaluation of flavonoids from Abrus precatorius leaves

Cited by 13 publications

References 25 publications

Potential Use of Saga Leaf Extract (Abrus precatorius) as Anti Bacteria Aeromonas hydrophila by in Vitro

Potential Use of Saga Leaf Extract (Abrus precatorius) as Anti Bacteria Aeromonas hydrophila by in Vitro

Quality Evaluation of Peony Petals Based on the Chromatographic Fingerprints and Simultaneous Determination of Sixteen Bioactive Constituents Using UPLC-DAD-MS/MS

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

Contact Info

Product

Resources

About