UPLC–MS/MS method for determination of selected pyrrolizidine alkaloids in feed

Bolechová, Martina; Čáslavský, Josef; Pospíchalová, Markéta; Kosubová, Petra

doi:10.1016/j.foodchem.2014.08.072

Cited by 40 publications

(27 citation statements)

References 24 publications

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“…8,9 PAs are among the most abundant group of natural toxins produced by plants. 10,11 Toxicity of individual PAs is related to the chemical structure and physical properties. 7 Cases of human exposure to PAs originate from direct consumption of PA-containing teas, herbal dietary and supplements.…”

Section: Introductionmentioning

confidence: 99%

Pyrrolizidine alkaloids quantified in soil and water using UPLC-MS/MS

Hama

Strobel

2019

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Pyrrolizidine alkaloids quantified in soil and water using UPLC-MS/MS

Hama

Strobel

2019

RSC Adv.

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“…Thus far, the only recent method that has been developed is an LC‐ESI‐MS/MS method that does not require clean‐up steps [limit of quantification (LOQ) of 0.3 ppb in honey samples] . An UPLC‐MS/MS method produced an LOD of 0.4 ppb for MCT determination in feed . Although this method is highly sensitive, both methods require costly devices and analytical procedures.…”

Section: Resultsmentioning

confidence: 99%

“…2 An UPLC-MS/MS method produced an LOD of 0.4 ppb for MCT determination in feed. 12 Although this method is highly sensitive, both methods require costly devices and analytical procedures. Rapidity, simplicity, and cost-performance are important factors for analytical methods, as well as sensitivity and specificity, when the methods are used in practical applications.…”

Section: Applicationmentioning

confidence: 99%

“…2 In addition, ultraperformance liquid chromatography (UPLC)-MS/MS and quadrupole time-of-flight (QTOF)-MS methods were reported to determine pyrrolizidine alkaloids in feed, botanicals, and dietary supplements. 11,12 Most analytical methods have been developed using MS-based detection; however, the cost and complexity of these methods has limited their application.…”

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confidence: 99%

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Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

Yusakul

Sakamoto

Chanpokapaiboon

et al. 2019

Phytochemical Analysis

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Introduction Monocrotaline (MCT), which is classified as a 1,2‐dehydropyrrolizidine alkaloid (DHPA), is a toxic compound that is mainly produced by Crotalaria spp. MCT contamination in cereals and herbs leads to hepatitis, gastroenteritis, pulmonary vasculitis and hypertension, and different types of cancer. The current analytical methods for MCT are complicated and expensive using liquid chromatography equipped with mass spectrometry detection. Objective The aim of this study was to develop a simple and sensitive preincubation format for an immunochromatographic assay (PI‐ICA) for MCT detection. Methodology We conducted the PI‐ICA via incubation of an MCT‐containing sample with an anti‐MCT monoclonal antibody conjugated with colloidal gold before strip dipping. We compared the PI‐ICA detection sensitivity with that of the conventional ICA (Conv‐ICA) format. Results The PI‐ICA was sensitive with a limit of detection (LOD) of 0.61 ng/mL, which is a 16‐fold improvement over the Conv‐ICA format. These results indicated that the PI‐ICA method exhibits high binding specificity for MCT and low cross‐reactivity towards retronecine, retrorsine, senecionine and heliotrine. Sample solutions from plants containing MCT and related DHPAs produced positive results via PI‐ICA analysis. Conclusions The proposed PI‐ICA system provides a highly sensitive method compared to Conv‐ICA. In addition, the developed PI‐ICA method is simple and highly effective for detecting MCT contamination.

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“…It has become one of the most important methods of pharmacokinetics (Jahne, Sampath, Butterweck, Hamburger, & Oufir, 2016;Li, Huang, Shao, Lin, & Shen, 2017;Och et al, 2017). UPLC-MS possesses the advantages of good separation performance of UPLC and giid discrimination of MS, resulting in a better analytical technique that can fully adapt to the new development of modern pharmaceutical research (Ai et al, 2015;Bolechova, Caslavsky, Pospichalova, & Kosubova, 2015).…”

Section: Introductionmentioning

confidence: 99%

Determination of armepavine in mouse blood by UPLC‐MS/MS and its application to pharmacokinetic study

Geng

Luo

Weng

et al. 2018

Biomedical Chromatography

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The purpose of this study was to develop an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to determine armepavine in mouse blood. Nuciferine was used as internal standard. Chromatographic separation was performed on a UPLC BEH (2.1 × 50 mm, 1.7 μm) column with a gradient elution of acetonitrile and 10 mmol/L ammonium acetate solution (containing 0.1% formic acid). The quantitative analysis was conducted in multiple reaction monitoring mode with m/z 314.1 → 106.9 for armepavine and m/z 296.2 → 265.1 for nuciferine. Calibration curves were linear (r > 0.995) over the concentration range 1-1000 ng/mL in mouse blood with a lowest limit of quantitation of 1 ng/mL. The intra- and inter-day precisions of armepavine in mouse were < 13.5 and 10.8%, respectively. The accuracy ranged between 86.8 and 103.3%. Meanwhile, the average recovery was >70.7% and the matrix effect was within the range 109.5-113.7%. All of the obtained data confirmed the satisfactory sensitivity and selectivity of the developed method which was then successfully applied to evaluate the pharmacokinetic behavior of armepavine in mouse for the first time. The bioavailability of armepavine in mouse was calculated to be 11.3%.

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UPLC–MS/MS method for determination of selected pyrrolizidine alkaloids in feed

Cited by 40 publications

References 24 publications

Pyrrolizidine alkaloids quantified in soil and water using UPLC-MS/MS

Pyrrolizidine alkaloids quantified in soil and water using UPLC-MS/MS

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

Determination of armepavine in mouse blood by UPLC‐MS/MS and its application to pharmacokinetic study

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