Upregulation of let-7a inhibits vascular smooth muscle cell proliferation in vitro and in vein graft intimal hyperplasia in rats

Cao, Hui; Hu, Xinhua; Qiang, Zhang; Wang, Junpeng; Li, Jun; Liu, Bing; Shao, Yang; Li, Xi; Zhang, Jian; Xin, Shijie

doi:10.1016/j.jss.2014.05.045

Cited by 21 publications

(12 citation statements)

References 49 publications

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“…The let-7 family plays an important role in VSMC function. Let-7a decreased the proliferation of cultured VSMCs by reducing the expression of c-Myc and KRAS and could prevent intimal hyperplasia in an experimental vein graft model [102]. Overexpression of let-7d reduces VSMC growth by targeting KRAS [103].…”

Section: Mirnas and Vascular Agingmentioning

confidence: 99%

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Lin

Zhan

Wang

et al. 2016

BioMed Research International

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Vascular aging, a specific type of organic aging, is related to age-dependent changes in the vasculature, including atherosclerotic plaques, arterial stiffness, fibrosis, and increased intimal thickening. Vascular aging could influence the threshold, process, and severity of various cardiovascular diseases, thus making it one of the most important risk factors in the high mortality of cardiovascular diseases. As endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) are the main cell biological basis of these pathology changes of the vasculature, the structure and function of ECs and VSMCs play a key role in vascular aging. MicroRNAs (miRNAs), small noncoding RNAs, have been shown to regulate the expression of multiple messenger RNAs (mRNAs) posttranscriptionally, contributing to many crucial aspects of cell biology. Recently, miRNAs with functions associated with aging or aging-related diseases have been studied. In this review, we will summarize the reported role of miRNAs in the process of vascular aging with special emphasis on EC and VSMC functions. In addition, the potential application of miRNAs to clinical practice for the diagnosis and treatment of cardiovascular diseases will also be discussed.

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Section: Mirnas and Vascular Agingmentioning

confidence: 99%

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Lin

Zhan

Wang

et al. 2016

BioMed Research International

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“…Venous stenosis of dialysis vascular access is histologically characterized by intimal thickening, mainly due to the proliferation of smooth muscle cells and myofibroblasts and deposition of extracellular matrix, and hence is different from atherosclerotic lesions. In a porcine vein graft model, Cao et al (25) found that six miRNAs were downregulated and ten miRNAs were method used in our study. This hybridization-based platform used high-throughput technology to analyze hundreds to thousands of miRNAs in one assay, but it may be considered to have lower dynamic range and specificity than the quantitative real-time PCR method (28).…”

Section: Discussionmentioning

confidence: 59%

MicroRNA-21 and Venous Neointimal Hyperplasia of Dialysis Vascular Access

Chen

Hsieh

et al. 2018

CJASN

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Background and objectives There is increasing evidence that microRNAs (miRNAs) play crucial roles in the regulation of neointima formation. However, the translational evidence of the role of miRNAs in dialysis vascular access is limited.Design, setting, participants, & measurements miRNA expression in tissues was assessed by using venous tissues harvested from ten patients on dialysis who received revision or removal surgery, and ten patients who were predialysis and received creation surgery of arteriovenous fistulas served as controls. To extend these findings, 60 patients who received angioplasty of dialysis access were enrolled and the levels of circulating miRNAs were determined before and 2 days after angioplasty. Clinical follow-up was continued monthly for 6 months. The primary outcome of angioplasty cohort was target lesion restenosis within 6 months after angioplasty. ResultsIn the surgery cohort, the expressions of miR-21, miR-130a, and miR-221 were upregulated in stenotic tissues, whereas those of miR-133 and miR-145 were downregulated. In situ hybridization revealed similar expression patterns of these miRNAs, localized predominantly in the neointima region. Twenty eight patients in the angioplasty cohort developed restenosis within 6 months. The levels of circulating miR-21, miR-130a, miR-221, miR-133, and miR-145 significantly increased 2 days after angioplasty. Kaplan-Meier plots showed that patients with an increase of miR-21 expression level .0.35 have a higher risk of patency loss (hazard ratio, 4.45; 95% confidence interval, 1.68 to 11.7). In a multivariable analysis, postangioplasty increase of miR-21 expression was independently associated with restenosis (hazard ratio, 1.20; 95% confidence interval, 1.07 to 1.35 per one unit increase of miR-21 expression level; P=0.001).Conclusions Certain miRNAs are differentially expressed in the stenotic venous segments of dialysis accesses. An increase in blood miR-21 level with angioplasty is associated with a higher risk of restenosis.

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“…According to recent significant research, many miRNAs, including miR‐24‐3p, miR‐424‐5p, miR‐1298‐5p, miR‐204‐5p and let‐7a‐5p, are involved in VSMC proliferation and down‐regulated in proliferative VSMCs. In the HASMCs, the MREs of the miRNAs mentioned above were predicted in DEcircRNAs.…”

Section: Discussionmentioning

confidence: 99%

Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells

Chen

Lin

et al. 2020

J Cellular Molecular Medi

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| INTRODUC TI ONCardiovascular diseases (CVD), including vascular remodelling diseases, are the leading cause of mortality among humans throughout the world. Abnormal proliferation of vascular smooth muscle cells (VSMCs) is a common feature of many vascular remodelling diseases, including atherosclerosis, 1 hypertension 2 and vascular aneurysms. 3 The regulation of VSMC proliferation has been considered a key event due to its major implications for the prevention of pathological vascular conditions. 4 Non-coding RNAs (ncRNAs) form the dominant product of eukaryotic transcription, comprising over 73% of the human genome. 5 Circular RNAs (circRNAs) are a special novel type of endogenous ncRNAs, forming covalently closed-loop structures without 5′ caps Abstract Vascular smooth muscle cell (VSMC) proliferation is the pathological base of vascular remodelling diseases. Circular RNAs (circRNAs) are important regulators involved in various biological processes. However, the function of circRNAs in VSMC proliferation regulation remains largely unknown. This study was conducted to identify the key differentially expressed circRNAs (DEcircRNAs) and predict their functions in human aortic smooth muscle cell (HASMC) proliferation. To achieve this, DEcircRNAs between proliferative and quiescent HASMCs were detected using a microarray, followed by quantitative real-time RT-PCR validation. A DEcircRNA-miRNA-DEmRNA network was constructed, and functional annotation was performed using Gene Ontology (GO) and KEGG pathway analysis. The function of hsa_circ_0002579 in HASMC proliferation was analysed by Western blot. The functional annotation of the DEcircRNA-miRNA-DEmRNA network indicated that the four DEcircRNAs might play roles in the TGF-β receptor signalling pathway, Ras signalling pathway, AMPK signalling pathway and Wnt signalling pathway. Twenty-seven DEcircRNAs with coding potential were screened. Hsa_circ_0002579 might be a pro-proliferation factor of HASMC. Overall, our study identified the key DEcircRNAs between proliferative and quiescent HASMCs, which might provide new important clues for exploring the functions of circRNAs in vascular remodelling diseases. K E Y W O R D S circular RNAs, coding potential, HASMCs, microRNA sponge, proliferation | 4763 CHEN Et al.

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Upregulation of let-7a inhibits vascular smooth muscle cell proliferation in vitro and in vein graft intimal hyperplasia in rats

Cited by 21 publications

References 49 publications

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

MicroRNA-21 and Venous Neointimal Hyperplasia of Dialysis Vascular Access

Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells

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