Uptake and intracellular stability of glycosylphosphatidylinositol-specific phospholipase D in neuroblastoma cells

Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7

“…Although these results may simply demonstrate the limitations of protein-based detection methods, they could also indicate that the reduction in mRNA level results only in a transient decrease in newly synthesized GPI-PLD in the endoplasmic reticulum, where the degradation of GPI anchors is proposed to occur in vivo (43). This change might not be large enough to affect the overall level of cellular GPI-PLD, the bulk of which may be stored in other cellular compartments (6,10,47).…”

Thematic review series: Lipid Posttranslational Modifications. Lysosomal metabolism of lipid-modified proteins

“…The activity has also been described as enriched in lysosomal fractions of bovine liver (44). GPI-PLD is taken up into cells from culture medium and proteolyzed to smaller fragments, a process that is inhibited by the lysosomotropic agent chloroquine (45), which raises the possibility of lysosomal targeting. Metabolism of GPI anchor intermediates, a function also performed by GPI-PLD, has been shown to be sensitive to chloroquine and ammonium chloride, maneuvers that increase lysosomal pH (46).…”