Urinary metabolomics study on the anti-inflammation effects of flavonoids obtained from Glycyrrhiza

Wei, Mengying; Yan, Ma; Liu, Yuanyuan; Zhou, Yuan; Men, Lihui; Yue, Kexin; Pi, Zifeng; Liu, Zhiqiang

doi:10.1016/j.jchromb.2018.04.007

Cited by 25 publications

(17 citation statements)

References 39 publications

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“…p ‐Cresol is a metabolite of tyrosine or phenylalanine and also a uremic toxin. Tyrosine and phenylalanine are converted to p ‐hydroxyphenylacetic acid by gut microbes and then decarboxylated to p ‐cresol (Wei et al, ). p ‐Cresol can be highly bound to proteins, producing uremic toxins that are difficult to separate (Meijers et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that taurine enhances the expression and activities of antioxidant enzymes (Sun, Piao, Cha, & Kim, ). Although taurine cannot be oxidized, studies have shown that it could be used as an antioxidant to protect organisms (Wei et al, ). Taurine combines with bile acid to produce taurocholic acid, which is involved in lipid and cholesterol metabolism and reduces bile acid accumulation (Gao, Wan, Zhuang, Wei, & Han, ).…”

Section: Resultsmentioning

confidence: 99%

“…Regarding DR, vitreous and serum samples are the main tissues studied (Liew et al, ). A variety of analytical techniques, such as gas chromatography–mass spectrometry, liquid chromatography–mass spectrometry (LC–MS) and nuclear magnetic resonance, have become powerful tools for metabolomics research (Wei et al, ; Zimny et al, ). Ultrahigh‐performance liquid chromatography coupled with Q‐exactive quadrupole–electrostatic field Orbitrap mass spectrometry (UPLC–Q‐exactive Orbitrap–MS) has the advantages of high resolution, good quality stability, high sensitivity and fast speed in complex matrix, which is a good option for metabolite analysis of TCM.…”

Section: Introductionmentioning

confidence: 99%

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Urine metabolomics study of Bushen Huoxue Prescription on diabetic retinopathy rats by UPLC–Q‐exactive Orbitrap–MS

Wang

Xie

et al. 2020

Biomedical Chromatography

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Diabetic retinopathy (DR) is a common microvascular complication of diabetes and remains one of the leading causes of blindness worldwide. Previous studies have shown that Bushen Huoxue Prescription (BP) possesses an effect on preventing and treating DR, but the mechanisms of action are not entirely clear. In order to clarify the mechanisms, the pharmacodynamical assessments of BP were investigated. Combining the pharmacodynamical studies, it was found that BP could inhibit high expression of VEGF and HIF‐1α, indicating significant therapeutic effects on DR. In order to further investigate the mechanism of BP in treating DR, a urine metabolomics method based on ultrahigh‐performance liquid chromatography coupled with Q‐exactive quadrupole‐electrostatic field Orbitrap mass spectrometry was established to observe the metabolic variations in DR rats and investigate the therapeutic effect of BP on DR. As a result, nine potential biomarkers associated with DR were found. The metabolic pathways related to these compounds were explored, and the results showed that these biomarkers were mainly associated with gut microbial metabolism, lipid metabolism and tryptophan metabolism. The results for the pharmacodynamics and metabolomics provided a theoretical basis for clarifying the mechanism of BP in the treatment of DR.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Urine metabolomics study of Bushen Huoxue Prescription on diabetic retinopathy rats by UPLC–Q‐exactive Orbitrap–MS

Wang

Xie

et al. 2020

Biomedical Chromatography

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“…Flavonoids constitute a large group of secondary metabolites that are widely distributed in plants, they play important roles in many biological processes, and possess several health-promoting and health-protecting properties due to their antioxidant, anticancer, antifungal and antiviral activities [1][2][3][4][5][6][7][8][9][10][11][12][13][14].…”

Section: Discussionmentioning

confidence: 99%

Whole-transcriptome Analysis Reveals the Promising Bioresource of Total Flavonoid from Bitter-Pit Apples, Especially the Pitted Parts

Xue

Wang

et al. 2020

Preprint

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Background: Apple fruits are rich in flavonoids, and play important roles in human-health protection against chronic diseases. However, pitter pit in apple has affected apple fruit production worldwide. There must be some application values could be exploited from the bitter-pit apples so as to reduce the loss caused by bitter pit.Results: In the present study, the influence of bitter pit on the total flavonoid content and flavonoid biosynthesis in apples was investigated using the aluminum chloride colorimetric method, whole-transcriptome sequencing and qRT-PCR analysis. The results showed that the total flavonoid content in bitter-pit apples (BG), pitted parts (BGBB) and non-pitted parts (JKBF) was 4.28-fold, 4.68-fold and 0.57-fold respectively as that in healthy apples (JKG). By RNA-Seq analysis, 26, 23 and 3 DEGs involved in flavonoid biosynthesis were enriched in JKG vs. BG, JKG vs. BGBB and JKG vs. JKBF comparisons, respectively. Eight DEGs [CYP98A3(1), CYP98A3(2), BADH, DAT, HCT(1), HCT(2), CHI(1) and CHI(2)], were selected to be validated by qRT-PCR analysis, and the consistent expression patterns with RNA-Seq analysis were obtained, the results showed that the 8 DEGs were upregulated in BG and BGBB but downregulated in JKBF when compared with JKG.Conclusions: The flavonoid accumulation and biosynthesis in apples, especially the pitted parts, were stimulated greatly by bitter pit, while depressed slightly in non-pitted parts. The results indicated that the bitter-pit apples, especially the pitted parts, could be used as the promising bioresource of total flavonoid for the therapeutic utilization in human chronic diseases.

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“…Previous studies have shown that G.glabra has anti-allergic and anti-inflammatory activities, and that it is used for the treatment of various diseases such as allergy, asthma, and eczema. In addition, G.glabra is known to be useful for the treatment of chronic hepatitis and viral infections caused by human viruses such as human immunodeficiency virus, cytomegalovirus, and Herpes simplex virus in Japan (32)(33)(34)(35)(36)(37)(38).…”

Section: Tetracyclinementioning

confidence: 99%

An experimental in-vitro study to evaluate the anti-helicobacter activity of Glycyrrhetinic acid

Çelik

Duran

2019

Revista Romana De Medicina De Laborator

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Aim: The aim of this study was to investigate the in-vitro efficacy of Glycyrrhetinic acid against Helicobacter pylori (H. pylori) strains, as compared with conventional antibacterial agents. Methods: A total of 41 H. pylori isolates were used, 6 of which were of standard strains (NCTC 1637), 8 of which were drug-sensitive, and 27 were resistant to drugs isolates. Clarithromycin and metronidazole resistance in all strains of H. pylori were determined by the Epsilometer test (E-test) method. MIC study was performed by using microdilution broth method. Results: Glycyrrhetinic acid was found to be effective against H. pylori NCTC 1637 in doses of 12.0±4.38 µg/mL, while the MIC value of clinical H. pylori isolates susceptible to antimicrobials was 20.8±10.11 µg/ml. It was found that the MIC values for antimicrobial-sensitive clinical H. pylori isolates was higher when compared with H. pylori NCTC 1637 strains. The MIC values of the standard antimicrobial agents against drug-resistant H. pylori strains were higher than H. pylori NCTC 1637 strains and drug-sensitive H. pylori strains. The MIC value was found to be 14.22±7.77 µg/ml for metronidazole, 3.89±1.90 µg/ml for clarithromycin, 2.33±1.0 µg/ml for amoxicillin, 2.44±0.88 µg/ml for levofloxacin and 4.89±2.47 µg/ml for tetracycline, whereas the MIC value of Glycyrrhetinic acid was 26.67±8.0 µg/ml in metronidazole-resistant H. pylori isolates. Besides, MIC values of the antimicrobials and 18ß-Glycyrrhetinic acid among the strains resistant to clarithromycin were as follows: 3.25±2.12 µg/ml for metronidazole, 9.71±4.54 µg/ml for clarithromycin, 2.06±1.32 µg/ml for amoxicillin, 3.88±4.22 µg/ml for levofloaxacin and 3.25±1.04 µg/mL for tetracycline and 22.0±11.11 µg/ml for Glycyrrhetinic acid. Conclusion: Glycyrrhetinic acid had significant antimicrobial activity against H. pylori strains. Although further in-vivo studies are needed on antimicrobial activity of Glycyrrhetinic acid, increased resistance to drugs currently used in treatment suggests that Glycyrrhetinic acid may be a potential agent for the treatment of H. pylori.

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Urinary metabolomics study on the anti-inflammation effects of flavonoids obtained from Glycyrrhiza

Cited by 25 publications

References 39 publications

Urine metabolomics study of Bushen Huoxue Prescription on diabetic retinopathy rats by UPLC–Q‐exactive Orbitrap–MS

Urine metabolomics study of Bushen Huoxue Prescription on diabetic retinopathy rats by UPLC–Q‐exactive Orbitrap–MS

Whole-transcriptome Analysis Reveals the Promising Bioresource of Total Flavonoid from Bitter-Pit Apples, Especially the Pitted Parts

An experimental in-vitro study to evaluate the anti-helicobacter activity of Glycyrrhetinic acid

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