Urolithin C, a gut metabolite of ellagic acid, induces apoptosis in PC12 cells through a mitochondria-mediated pathway

Yin, Peng; Zhang, Jianwei; Yan, Linlin; Yang, Lingguang; Sun, Liwei; Shi, Lingling; Ma, Chao

doi:10.1039/c7ra01548h

Cited by 24 publications

(16 citation statements)

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“…In diabetes, chronic hyperglycemia can induce carbonyl stress, which would lead to the increase of lipid peroxidation [ 42 ]. MDA is one of the most prevalent byproducts of lipid peroxidation during oxidative stress, therefore the content of MDA could be used as an index of the lipid peroxidation degree [ 15 , 43 ]. As listed in Table 3 , no significant difference of MDA levels was observed in normal rats without or with ECE treatments (NC, N + E2 and N + E8), and alloxan induction (DC) upraised MDA levels with no significant difference only in spleen compared to that of the NC rats.…”

Section: Resultsmentioning

confidence: 99%

Hypoglycemic Effects in Alloxan-Induced Diabetic Rats of the Phenolic Extract from Mongolian Oak Cups Enriched in Ellagic Acid, Kaempferol and Their Derivatives

et al. 2018

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Our previous reports showed that crude extract prepared with 50% ethanol (ethanol crude extract, ECE) from Mongolian oak cups possessed excellent in vitro antioxidant capacities as well as inhibitory activities against α-glucosidase, α-amylase and protein glycation caused by its enrichment in phenolics, including mainly ellagic acid, kaempferol and their derivatives. Nevertheless, few in vivo studies on antidiabetic activities of these phenolics were conducted. The present study investigated hypoglycemic effects with normal and diabetic rats being administrated orally without or with ECE at 200 and 800 mg/kg for 15 days. In normal rats, no significant differences were exhibited after ECE administration in body weight, fasting blood glucose level, levels of cholesterol, triglyceride, LDL and AST in serum, organ indexes, and levels of GSH and MDA in organs. In diabetic rats, the fasting blood glucose level, indexes of heart and liver, and levels of cholesterol and triglyceride in serum and MDA in heart tissue were significantly decreased. Moreover, HDL levels in serum and SOD activities in the four organs of diabetic rats were significantly improved after ECE administration at 800 mg/kg. Thus, in addition to inhibiting α-glucosidase, α-amylase and protein glycation reported previously, oak cups might contain novel dietary phytonutrients in preventing abnormal changes in blood glucose and lipid profile and attenuating oxidant stress in vivo. The results also implied that it is ellagic acid, kaempferol and their derivatives enriched in ECE that might play vital roles in managing type 1 as well as type 2 diabetes.

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Section: Resultsmentioning

confidence: 99%

Hypoglycemic Effects in Alloxan-Induced Diabetic Rats of the Phenolic Extract from Mongolian Oak Cups Enriched in Ellagic Acid, Kaempferol and Their Derivatives

et al. 2018

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“…To further assess how L. plantarum Y44 increased cell viability of HT-29 cells injured by H 2 O 2 , the ex- pression of Bcl-2, Bax, and Hsp70 were evaluated by Western blotting analysis. It is well known that Bax and Bcl-2 have been shown to play an important role in the regulation of mitochondria-mediated cell apoptosis (Huang et al, 2015;Yin et al, 2017). As shown in Figure 8B and 8D, the Bax expression of the HT-29 cells injured by H 2 O 2 alone was upgrading about 1.24-fold (P < 0.05), Bcl-2 expression was downgrading about 0.7-fold (P < 0.05), indicating that H 2 O 2 induced HT-29 cell apoptosis.…”

Section: Protection Of Lactobacilli Strains For Ht-29 Cells Against H 2 O 2 Injurymentioning

confidence: 99%

Assessing and comparing antioxidant activities of lactobacilli strains by using different chemical and cellular antioxidant methods

Gao

Tuo

et al. 2018

Journal of Dairy Science

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Some lactobacilli strains had beneficial effects on human beings due to their antioxidant activities. In this study lactobacilli strains stored in our laboratory were screened for potential antioxidant activities by investigating their 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, oxygen radical absorbance capacity, resistance to H 2 O 2 , and hydroxyl free radical scavenging activity; then the antioxidant activities of the screened strains were evaluated by cellular antioxidant assay and protection for HT-29 cells against H 2 O 2 injury assay. The results showed that Lactobacillus plantarum Y44 could scavenge oxygen free radicals, inhibit the production of intracellular reactive oxygen species without creating obvious cytotoxic effects, and protect HT-29 cells against H 2 O 2 injury evidenced by the significant decrease of the Bcl-2-associated X protein (Bax)/B-cell lymphoma 2 (Bcl-2) ratio and heat shock protein 70 expression, increase of superoxide dismutase and glutathione peroxidase activities, and decrease of malondialdehyde level of HT-29 cells damaged by H 2 O 2 . It was speculated that L. plantarum Y44 protect HT-29 cells against oxygen radical injury through scavenging reactive oxygen species and activating intracellular antioxidant enzymes. A significant correlation was observed among the results of the hydroxyl radical scavenging assay, protection assay for HT-29 cells against H 2 O 2 injury, and the cellular antioxidant assay. The findings indicated that L. plantarum Y44 could be a probiotic candidate with antioxidant properties and combining several chemical antioxidant methods and antioxidant cellular models could be an effective procedure to screen lactobacilli strains with antioxidant activity.

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“…To examine the effects of RA‐rich extract on the apoptosis rate of H 2 O 2 ‐pretreated HepG2 cells, Annexin V–fluorescein isothiocyanate/propidium iodide double staining was performed to distinguish apoptotic from damaged cells. The total apoptosis rate was calculated as the sum of apoptosis rates of cells in the upper‐right and lower‐right quadrants (Yin et al, ). The percentage of apoptotic cells in the H 2 O 2 ‐pretreated group was ∼39.5%, considerably higher than that in normal cells (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Protective effect of a rosmarinic acid-rich extract from cold-pressedPerilla frutescensseed flour on oxidative hepatotoxicity in vitro and in vivo

et al. 2018

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Abundant phenolic compounds, including rosmarinic acid (RA), caffeic acid, and their derivatives, are present in cold-pressed Perilla frutescens seed flour (CP-PFSF) after oil extraction. In this study, the in vitro antioxidant and hepatoprotective activities of the major phenolic components of CP-PFSF were assessed and compared. RA showed the highest antioxidant and hepatoprotectiveactivities. An RA-rich extract was prepared and its hepatoprotective effects were assessed in vitro and in vivo. In vitro, treatment with RA-rich extract markedly reduced H 2 O 2 -induced cytotoxicity, significantly prevented H 2 O 2 -induced lipid peroxidation and glutathione depletion, inhibited H 2 O 2induced apoptosis by regulating the expression of Bcl-2/Bax, caspase 9, and caspase 3, which are involved in the mitochondrial apoptosis pathway. In vivo, oral administration of RA-rich extract significantly reduced the levels of aspartate aminotransferase and alanine aminotransferase, and hepatocyte degeneration and neutrophilic infiltration induced by tert-butylhydroperoxide (t-BHP).

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Urolithin C, a gut metabolite of ellagic acid, induces apoptosis in PC12 cells through a mitochondria-mediated pathway

Abstract: Urolithin C includes apoptosis in PC12 cells through a mitochondria-mediated pathway.

Cited by 24 publications

References 55 publications

Hypoglycemic Effects in Alloxan-Induced Diabetic Rats of the Phenolic Extract from Mongolian Oak Cups Enriched in Ellagic Acid, Kaempferol and Their Derivatives

Hypoglycemic Effects in Alloxan-Induced Diabetic Rats of the Phenolic Extract from Mongolian Oak Cups Enriched in Ellagic Acid, Kaempferol and Their Derivatives

Assessing and comparing antioxidant activities of lactobacilli strains by using different chemical and cellular antioxidant methods

Protective effect of a rosmarinic acid-rich extract from cold-pressedPerilla frutescensseed flour on oxidative hepatotoxicity in vitro and in vivo

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