Use of Accurate Mass Full Scan Mass Spectrometry for the Analysis of Anthocyanins in Berries and Berry-Fed Tissues

Abstract

Anthocyanins in extracts from raspberries and blueberries were analyzed by reversed-phase HPLC coupled to a high-resolution Exactive Orbitrap mass spectrometer (HR-MS) with a resolution of 100,000, operated with an electrospray source in the positive ionization mode. As consumption of anthocyanin-rich berry extracts has been associated with improved cognitive function, brain extracts from European greenfinches ( Carduelis chloris ) that had been fed one blackberry daily for a period of 2 weeks were analyzed by both HPLC with traditional tandem MS in the selected reaction monitoring mode and HPLC-HR-MS. Cyanidin-3-O-glucoside was detected in the brain extracts by both methods, but because of its high level of selectivity, HR-MS was ca. 200-fold more sensitive. A further advantage of HR-MS is that unlike MS-SRM it enables both targeted and nontargeted compounds to be detected and much lower limits of detection are achieved without compromising the selectivity of the analysis.

Evaluation of Antioxidant Activities of Some Small Fruits Containing Anthocyanins Using Electrochemical and Chemical Methods

“…The most used technique for separation, identification and quantification of anthocyanins is high performance liquid chromatography (HPLC) coupled with UV-Vis, photodiode array (PDA) or mass spectrometry (MS) detectors. [11][12][13][14] Small berry fruits like chokeberry, elderberries, blackcurrants, bilberry, etc.…”

Systematic bottom-up approach for flavonoid derivative screening in plant material using liquid chromatography high-resolution mass spectrometry

“…Attraction towards mass spectrometry (MS)-based profiling methods of flavonoids has also increased in the recent years [12,[16][17][18][19]. Mass spectrometry usually with electrospray ionization often combined with a diode array detector appeared to be the most important technique for flavonoid profiling.…”

“…The specificity obtained from in-source fragmentation is not at the level of dedicated MS/MS experiments, but this can be overcome by using highresolution and accurate mass measurements of the ions of interest. For this purpose, MS systems equipped with mass analyzers capable of producing accurate mass measurements such as time-of-flight (TOF) [7,[26][27][28], Orbitrap [19], or Fourier transform-ion cyclotron resonance [29] have been used in the field of flavonoid profiling.…”

Determination of cyanidin-3-glucoside (red kernel food colour) in beverages by high performance liquid chromatography and a study of its degradation by quadruple time-of-flight mass spectrometry

“…Current research has focused on extraction technologies for AC, such as supercritical solvent extraction (Monrad et al 2010), ultrasonics, high hydrostatic pressure or pulsed electric fields (Corrales et al 2008), and characterisation of the extracts (Hong and Wrolstad 1990;Pazmin˜o-Dura´n et al 2001;AbadGarcı´a et al 2009;Mullen et al 2010;Truong et al 2010;Yang and Zhai 2010) for AC pigments. Analytical methods for AC are mainly limited to qualitative determination of the components of the extracts from various food sources by HPLC (Hong and Wrolstad 1990), LC-low resolution mass spectrometry (LR-MS) (Pazmin˜o-Dura´n et al 2001;AbadGarcı´a et al 2009;Truong et al 2010;Yang and Zhai 2010) and LC-high resolution mass spectrometry (HR-MS) (Mullen et al 2010). Chandra et al (2001) established the quantification of individual as well as total AC components by HPLC and LC-MS with cyanidin 3-glucoside (Cyd-3-Glu) as external standard and expressed as its equivalents.…”