Use of chitosan membrane associated with polypropylene mesh to prevent peritoneal adhesion in rats

Abstract

The correction of wall abdominal defects often requires the use of implants such as polypropylene meshes. In spite of presenting good tissue acceptance, these biomaterials can migrate to adjacent viscera, promote enterocutaneos fistulas, tissue adherence and visceral erosions. In this work, the barrier effect of chitosan films associated with polypropylene meshes on adhesion formation experimentally induced in Wistar rats was evaluated. The animals were divided into two groups with 10 animals each. Animals in the CPP group were implanted with chitosan films associated with polypropylene meshes, whereas the ones in the PP group received only polypropylene meshes. After 8 days, the animals were submitted to euthanasia using CO(2) and a descriptive study focusing adhesion formation, visceral involvement with sutures and mesh peritonization was performed. Also, subimplanted material was collected for histopathology analysis. The results showed that the CPP group presented weak adhesions to the omentum over the stitch knots in eight animals. In all animals, the meshes were peritonized, not allowing their visualization after removing the chitosan films. In the PP group, six animals presented intestinal adhesions to the meshes and, in one of them, hepatic adhesion to the mesh was observed, besides omentum adhesion on more than 50% of the mesh area. The protective effect of chitosan films when sutured over polypropylene meshes, as well as no exacerbation of inflammation associated to the peritoneal lesions was statistically demonstrated. Therefore, chitosan films can indeed minimize the formation of peritoneal adhesions induced by polypropylene meshes in rats.

A Comparative Study of the Preventive Effects of Mitomycin C and Chitosan on Intraarticular Adhesion after Knee Surgery in Rabbits

“…Mitomycin C(MMC) and chitosan are known to inhibit the proliferation of fibroblast and reduce tissue adhesion. At present, both MMC and chitosan are being used against tissue adhesion in clinical and experimental research and a successful outcome was reported [8][9][10].…”

Bending of Layer-by-Layer Films Driven by an External Magnetic Field

“…The wide variety of new nanomaterials with multifunctional properties has sparked studies aimed at biological applications such as artificial muscles [1–4], drug delivery [5–8], implants [9,10] and drug therapies within the nanomedicine paradigm [11,12]. Very stringent requirements must be satisfied for such applications, especially with regard to biocompatibility and/or biodegradability, in addition to the need of suitable mechanical properties.…”

Bilaminar Chitosan Scaffold for Sellar Floor Repair in Transsphenoidal Surgery

“…The use of chitosan [poly-(β-1/4)-2-amino-2-deoxy-Dglucopyranose] has been described in several preclinical studies and tested in tissue bioengineering of bone (Zhao et al, 2002;Kim et al, 2004;Yoshida et al, 2004;Teng et al, 2008;Yuan et al, 2008;Liu et al, 2009;Xianmiao et al, 2009;Li et al, 2010;Mota et al, 2012;Pu et al, 2012;Zhang et al, 2012;Azevedo et al, 2014;Fan et al, 2014;Rodriguez-Vázquez et al, 2015;Vega-Ruiz et al, 2017) neural tissue (Simoes et al, 2011;Meyer et al, 2016;Ghasemi Hamidabadi et al, 2017;Nawrotek et al, 2017;Zhao et al, 2017) and soft tissue (Gobin et al, 2006;Paulo et al, 2009;Tchemtchoua et al, 2011;Udpa et al, 2013;Zou et al, 2017). Chitosan is a copolymer derived from the alkaline deacetylation of chitin and made of N-acetyl-D-glucosamine and D-glucosamine bonds and β bonds in which glucosamine is the main repeating unit in its structure (Rodriguez-Vázquez et al, 2015).…”