A Comparative Study of the Preventive Effects of Mitomycin C and Chitosan on Intraarticular Adhesion after Knee Surgery in Rabbits

Wang, Jingcheng; Yan, Lianqi; Sun, Yu; Wang, Daxin; Dai, Shanhe; Yu, Tangyun; Gu, Jiaxiang; Jiang, Baichuan; Feng, Xinmin; Hu, Hansheng; Wang, Qiang; Yin, Bin; Lv, Guohua

doi:10.1007/s12013-011-9266-5

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…Intra-articular adhesion leads to joint fibrosis and arthrosclerosis, reduces the range of knee joint movement and even causes disability [28]. Although the pathophysiological mechanism of the formation of knee intra-articular adhesion is not fully understood, fibroblastic activity and an inflammatory response are reported to be involved in the development of intra-articular adhesions [29,30].…”

Section: Discussionmentioning

confidence: 99%

Reduction of adhesion formation after knee surgery in a rat model by botulinum toxin A

Gao

Liu

et al. 2017

Bioscience Reports

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Adhesion of the knee is a major concern after knee surgery, the treatment of which is difficult. Botulinum toxin A (BTX-A) injection is demonstrated as efficient in treating knee adhesion after surgery. However, the treatment outcomes and the mechanism of action are not yet determined. The aim of the present study was to examine the effects and molecular mechanism of a BTX-A treatment in preventing adhesion of the knee. Twenty-four Wistar rats were randomly divided into a BTX-A treatment group and a control group. BTX-A or saline was injected into the cavity of the knee in the BTX-A treatment or control group respectively. Gross and histopathological examinations of interleukin 1 (IL-1) and fibroblast growth factor (FGF) levels, as well as fibroblast cell numbers, were assessed in the knee intra-articular adhesions in each group 6 weeks after recovery from the surgery. Macroscopic observations showed a significant reduction in adhesion severity in the BTX-A treatment group compared with the control group. In addition, the levels of IL-1 and FGF were lower and the number of fibroblasts was smaller in the BTX-A treatment group compared with those in the control group. BTX-A prevented intra-articular adhesion of knee in the rats, which might be associated with reduced expressions of IL-1 and FGF.

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Section: Discussionmentioning

confidence: 99%

Reduction of adhesion formation after knee surgery in a rat model by botulinum toxin A

Gao

Liu

et al. 2017

Bioscience Reports

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“…The animal model of intraarticular adhesion was developed in the knee of NewZealand White rabbits according to previous study615. After general anesthesia by intravenous administration of 2% pentobarbital (1.5 ml/kg), the femoral condyle of the left femur was exposed through a medial parapatellar approach.…”

Section: Methodsmentioning

confidence: 99%

The optimal concentration of topical hydroxycamptothecin in preventing intraarticular scar adhesion

Liang

Sun

et al. 2014

Sci Rep

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10-Hydroxycamptothecin could reduce intraarticular adhesion by inhibiting fibroblasts proliferation after knee surgery. However, the ideal concentration of hydroxycamptothecin have not been defined. This study was tried to verify the optimal concentration of 10-hydroxycamptothecin in preventing knee intraarticular adhesion. Sixty rabbits were randomly divided into five groups. Approximately 10 mm × 10 mm of the cortical bone was removed from both sides of the femoral condyle and the underneath cancellous bone was exposed. Various concentrations of hydroxycamptothecin (0.1 mg/ml, 0.5 mg/ml, 1.0 mg/ml, 2.0 mg/ml) or saline were applied to the decorticated areas for 10 minutes. After four weeks, the degree of inraarticular adhesion was assessed by macroscopic evaluation, biochemical analysis of hydroxyproline content and histological evaluation. The results demonstrated that the extent of knee inraarticular adhesion in 1.0 mg/ml group and 2.0 mg/ml hydroxycamptothecin group were significantly lower than those of 0.5 mg/ml group, 0.1 mg/ml hydroxycamptothecin group and control group. Moreover, there was no significant difference between 1.0 mg/ml group and 2.0 mg/ml hydroxycamptothecin group. In conclusion, topical application of 1.0 mg/ml hydroxycamptothecin may be the optimal concentration in reducing intraarticular adhesion after knee surgery in rabbits.

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“…In a clinical context, MMC is generally used as an anti-cancer drug (Kahmann et al 2010). However, it also finds application pre-clinically and clinically as an anti-fibrotic drug for keloid, capsular contracture, and scarring after ocular surgery (Lane et al 2003, Li et al 2013b, Nava et al 2017, Sidle and Kim 2011, Simman et al 2003, Wang et al 2012. MMC can attenuate the development of fibrosis as well as inhibit fibroblast proliferation in injury-induced intra-articular adhesion models (Kocaoglu et al 2011, Li et al 2013b, Wang et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Intra-Articular Injection of Mitomycin C Prevents Progression of Immobilization-Induced Arthrogenic Contracture in the Remobilized Rat Knee

2020

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This study tested whether cell cycle inhibitor mitomycin C (MMC) prevents arthrogenic contracture progression during remobilization by inhibiting fibroblast proliferation and fibrosis in the joint capsule. Rat knees were immobilized in a flexed position to generate flexion contracture. After three weeks, the fixation device was removed and rat knees were allowed to freely move for one week. Immediately after and three days after fixator removal, rats received intra-articular injections of MMC or saline. The passive extension range of motion (ROM) was measured before and after myotomy of the knee flexors to distinguish myogenic and arthrogenic contractures. In addition, both cellularity and fibrosis in the posterior joint capsule were assessed histologically. Joint immobilization significantly decreased ROMs both before and after myotomy compared with untreated controls. In saline-injected knees, remobilization increased ROM before myotomy, but further decreased that after myotomy compared with that of knees immediately after three weeks of immobilization. Histological analysis revealed that hypercellularity, mainly due to fibroblast proliferation, and fibrosis characterized by increases in collagen density and joint capsule thickness occurred after remobilization in saline-injected knees. Conversely, MMC injections were able to prevent the remobilization-enhanced reduction of ROM after myotomy by inhibiting both hypercellularity and joint capsule fibrosis. Our results suggest that joint capsule fibrosis accompanied by fibroblast proliferation is a potential cause of arthrogenic contracture progression during remobilization, and that inhibiting fibroblast proliferation may constitute an effective remedy.

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A Comparative Study of the Preventive Effects of Mitomycin C and Chitosan on Intraarticular Adhesion after Knee Surgery in Rabbits

Cited by 19 publications

References 23 publications

Reduction of adhesion formation after knee surgery in a rat model by botulinum toxin A

Reduction of adhesion formation after knee surgery in a rat model by botulinum toxin A

The optimal concentration of topical hydroxycamptothecin in preventing intraarticular scar adhesion

Intra-Articular Injection of Mitomycin C Prevents Progression of Immobilization-Induced Arthrogenic Contracture in the Remobilized Rat Knee

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