Use of cultured fibroblasts to restore skin in patients with severe burns

Abstract

A novel and unique method for treating skin burns by grafting cultured cells to the wound surface is described. The major graft elements are allogeneic human fibroblasts rather than keratinocytes. Experience with its use in 222 severely burned patients, including those after early surgical necrectomy, showed this method to be an effective means of treating "borderline" third-degree burns. Epithelialization times were shortened from 31+2 days to 8.4+0.9 days. When the method was combined with dermatoautoplasty using a 1:6 perforated netted skin flap, the epithelialization period decreased from 20+2.3 to 12+1.3 days. The method proved to be highly effective in the treatment of slowly healing wounds in donor areas. It is concluded that the advantages of the proposed method -high efficacy, the much lower costs (in comparison with other methods) because no expensive nutrient media or growth biostimulators have to be used, and the very short time required to obtain a graft from allogeneic fibroblasts -argue for its wide use in clinical practice.

Comparison between fibroblasts and mesenchymal stem cells derived from dermal and adipose tissue

“…The application of fresh human fibroblast allografts was found to be a safe and effective treatment for diabetic foot ulcers and for restoring skin with severe burns , as well as for cosmetic skin repairs .…”

The possibilities of using bioengineered skin substitutes in combustiology (literature review)

“…Вишневского представили ряд работ об использовании культивированного эпителия при лечении ожогов [46]. В 1990х годах были опубликованы результаты успешного культи вирования фибробластов [6]. В работе Е. Ал пеева и соавт.…”

Morphofunctional Evaluation of the Effect of Transplantation of Allogeneic Fibroblasts on Healing of Burn Wounds in Albino Outbred Mice and Mutant Hr hr /Hr hr Mice

“…Transplantation of allogeneic fi broblasts (AF) for the treatment of burn wounds is long and successfully used in clinical practice [3,4,9]. However, the mechanisms of the stimulating effects of AF on reparative regeneration of the skin after burn injury is poorly studied [2,5].…”