Use of flower‐like gold nanoparticles in time‐of‐flight mass spectrometry

Kolářová, Lenka; Kučera, Lukáš; Vaňhara, Petr; Hampl, Aleš; Havel, Josef

doi:10.1002/rcm.7265

Cited by 22 publications

(10 citation statements)

References 39 publications

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“…Sample preparation and reaction conditions were described in previous work [22]. Both flower-like and polyhedral AuNPs were collected by centrifugation at 16,000 g for 15 min at 21°C, washed several times with water, and resuspended at the desired concentration (1.6 mM for flower-like AuNPs and 0.24 mM for polyhedral AuNPs) in double-distilled water.…”

Section: Gold Nanoparticles and Sample Preparation For Msmentioning

confidence: 99%

“…Gold clusters provide simple and fast determination of accurate mass compared with common calibration standards, such as peptides or proteins. Moreover, AuNPs can enhance ionization in various techniques, such as surface-assisted laser desorption ionization (SALDI), nanoparticle-assisted laser desorption ionization (NALDI), and gold nanoparticle-assisted laser desorption ionization (GALDI) [20][21][22]. The structure and properties of gold and phosphorus clusters are well known [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, they did not provide a detailed evaluation and statistical analysis. Recently, we demonstrated that gold clusters generated from flower-like AuNPs can provide a precise internal calibration standard in SALDI analysis of peptides [22]. That work caused us to ask whether gold clusters are suitable for precise mass determination and the subsequent calibration of TOF analyzers.…”

Section: Introductionmentioning

confidence: 99%

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Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry

Kolářová

Prokeš

Kučera

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Precise calibration in TOF MS requires suitable and reliable standards, which are not always available for high masses. We evaluated inorganic clusters of the monoisotopic elements gold and phosphorus (Au n + /Au n -and P n + /P n -) as an alternative to peptides or proteins for the external and internal calibration of mass spectra in various experimental and instrumental scenarios. Monoisotopic gold or phosphorus clusters can be easily generated in situ from suitable precursors by laser desorption/ionization (LDI) or matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Their use offers numerous advantages, including simplicity of preparation, biological inertness, and exact mass determination even at lower mass resolution. We used citrate-stabilized gold nanoparticles to generate gold calibration clusters, and red phosphorus powder to generate phosphorus clusters. Both elements can be added to samples to perform internal calibration up to mass-to-charge (m/z) 10-15,000 without significantly interfering with the analyte. We demonstrated the use of the gold and phosphorous clusters in the MS analysis of complex biological samples, including microbial standards and total extracts of mouse embryonic fibroblasts. We believe that clusters of monoisotopic elements could be used as generally applicable calibrants for complex biological samples.

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Section: Gold Nanoparticles and Sample Preparation For Msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry

Kolářová

Prokeš

Kučera

et al. 2016

J. Am. Soc. Mass Spectrom.

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“…Among various metal-or metal oxide-based nanomaterials (e.g., Au [51,52,53,54,55], Ag [56], Pt [57,58,59], TiO 2 [60,61,62], ZnO [63], CdS [64], and HgTe [65] NPs) that explored as matrices for LDI-TOF MS, TiO 2 and gold NPs (Au NPs) attracted much more attention because their intrinsic advantages, such as large surface area, strong absorption efficiency in UV-visible region, high chemical stability, easy modification and preparation.…”

Section: Nanomaterial-assisted Ldi Methods Developmentmentioning

confidence: 99%

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

Yang

et al. 2017

Nanomaterials

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Matrix-assisted laser desorption/ionization (MALDI), a soft ionization method, coupling with time-of-flight mass spectrometry (TOF MS) has become an indispensible tool for analyzing macromolecules, such as peptides, proteins, nucleic acids and polymers. However, the application of MALDI for the analysis of small molecules (<700 Da) has become the great challenge because of the interference from the conventional matrix in low mass region. To overcome this drawback, more attention has been paid to explore interference-free methods in the past decade. The technique of applying nanomaterials as matrix of laser desorption/ionization (LDI), also called nanomaterial-assisted laser desorption/ionization (nanomaterial-assisted LDI), has attracted considerable attention in the analysis of low-molecular weight compounds in TOF MS. This review mainly summarized the applications of different types of nanomaterials including carbon-based, metal-based and metal-organic frameworks as assisted matrices for LDI in the analysis of small biological molecules, environmental pollutants and other low-molecular weight compounds.

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“…Ligand‐free AuNP were reported by Amendola et al 25 that exhibited low background levels in the low mass range ( m/z < 500) as compared to AuNP with surface‐protected agents, providing picomolar level detection levels for small molecules such as arginine, fructose, atrazine, anthracene, and paclitaxel. Flower‐like gold nanoparticles were used by Havel et al as mediators to enhance desorption/ionization of peptides 26 . The authors illustrated that the ion intensities for the peptides were up to 7.5‐fold higher using gold nanoflowers as substrate as compared to α‐cyanohydroxycinnamic acid (CHCA) as MALDI matrix.…”

Section: Introductionmentioning

confidence: 99%

Influence of core size and capping ligand of gold nanoparticles on the desorption/ionization efficiency of small biomolecules in AP‐SALDI‐MS

Liu

Zhang

Pyttlik

et al. 2020

Analytical Science Advances

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Gold nanoparticles (AuNP) are frequently used in surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) for analysis of biomolecules because they exhibit suitable thermal and chemical properties as well as strong surface plasmonic effects. Moreover, the structures of AuNP can be controlled by well-established synthesis protocols. This was important in the present work, which studied the influence of the nanoparticles' structures on atmospheric pressure (AP)-SALDI-MS performance. A series of AuNP with different core sizes and capping ligands were investigated, to examine the desorption/ionization efficiency (DIE) under AP-SALDI conditions. The results showed that both the AuNP core size as well as the nature of the surface ligand had a strong influence on DIE. DIE increased with the size of the AuNP and the hydrophobicity of the ligands. Chemical interactions between ligand and analytes also influenced DIE. Moreover, we discovered that removing the organic ligands from the deposited AuNP substrate layer by simple laser irradiation prior to LDI further amplified DIE values. The optimized AuNP were successfully used to analyze a wide arrange of different low molecular weight biomolecules as well as a crude pig brain extract, which readily demonstrated the ability of the technique to detect a wide range of lipid species within highly complex samples. 1 INTRODUCTION Since cobalt nanoparticles were first used for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) by Tanaka et al, 1 nanomaterials made from carbon, 2-4 silicon, 5-7 metals (Au, Ag, Pa, etc.), 8-10 metal oxides, 11-13 and others materials 14-17 have been successfully applied to SALDI-MS. In general, nanoparticles are excellent substrate options for SALDI, because they require only simple and easy sample preparation techniques and the materials often exhibit minimal background signals-in particular in the low m/z range-as compared to matrix-assisted laser desorption/ionization (MALDI). 18-20 SALDI efficiencies can be enhanced by optimizing the nanoparticular structures, for example, by modifying the core size and nature of the surface ligands, 18 which was shown to be vital for sensitive quantitative analysis of biomolecules. 20 In previous studies, it was found that desorption/ionization efficiency of SALDI was highly dependent on physical (size, surface roughness, light absorption, electrical conductivity, melting point, etc.) and chemical (surface modification, binding energy to analytes, etc.) properties of the nanomaterials. 18-23 Gold nanoparticles (AuNP) exhibit excellent electrical conductivities, thermal and chemical stabilities and strong light This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. c

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Use of flower‐like gold nanoparticles in time‐of‐flight mass spectrometry

Cited by 22 publications

References 39 publications

Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry

Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

Influence of core size and capping ligand of gold nanoparticles on the desorption/ionization efficiency of small biomolecules in AP‐SALDI‐MS

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