Use of Glucosamine and Chondroitin Sulfatein the Management of Osteoarthritis

Brief, Andrew A.; Maurer, Stephen G.; Cesare, Paul E. Di

doi:10.5435/00124635-200103000-00001

Cited by 52 publications

(27 citation statements)

References 18 publications

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“…Additionally, several studies have suggested that some substances, including supplement of collagen hydrolyzate, may be capable of repairing damaged articular cartilage or at least decelerating its progressive degeneration (Kelly 1998;Moskowitz 2000;Brief et al 2001;Oesser and Seifert 2003;Bello and Oesser 2006).…”

Section: Introductionmentioning

confidence: 98%

Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells

Raabe

Reich

Wenisch

et al. 2010

Histochem Cell Biol

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Adipose-derived stromal cells (ADSCs) are multipotent cells which, in the presence of appropriate stimuli, can differentiate into various lineages such as the osteogenic, adipogenic and chondrogenic. In this study, we investigated the effect of transforming growth factor beta 1 (TGF-β1) in comparison to hydrolyzed fish collagen in terms of the chondrogenic differentiation potential of ADSCs. ADSCs were isolated from subcutaneous fat of horses by liposuction. Chondrogenesis was investigated using a pellet culture system. The differentiation medium was either supplemented with TGF-β1 (5 ng/ml) or fish collagen (0.5 mg/ml) for a 3 week period. After the 3 weeks in vitro differentiation, RT-PCR and histological staining for proteoglycan synthesis and type II collagen were performed to evaluate the degree of chondrogenic differentiation and the formation of cartilaginous extracellular matrix (ECM). The differentiation of ADSCs induced by TGF-β1 showed a high expression of glycosaminoglycan (GAG). Histological analysis of cultures stimulated by hydrolyzed fish collagen demonstrated an even higher GAG expression than cultures stimulated under standard conditions by TGF-β1. The expression of cartilage-specific type II collagen and Sox9 was about the same in both stimulated cultures. In this study, chondrogenesis was as effectively induced by hydrolyzed fish collagen as it was successfully induced by TGF-β1. These findings demonstrated that hydrolyzed fish collagen alone has the potential to induce and maintain ADSCs-derived chondrogenesis. These results support the application of ADSCs in equine veterinary tissue engineering, especially for cartilage repair.

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Section: Introductionmentioning

confidence: 98%

Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells

Raabe

Reich

Wenisch

et al. 2010

Histochem Cell Biol

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“…49 Collagen fibers function to create a natural frame support for the ECM to resist mechanical stresses, whereas highly hydratable PGs fill the void spaces between the structural collagen fibers creating stress-transmitting paths to minimize tissue deformation. 50 In addition to the viscous components (mainly PGs and GAGs), the elastic components also contribute to the energy absorbing and storing capacity of the soft tissues. The effect of modified or reduced ECM components is shown with peak strain analysis where the acellular NaCl treated scaffolds reached higher strain levels (at 2 N) compared to Triton X-100 treated scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, tissue sections were fixed in 1% (v/v) glutaraldehyde (Sigma, St. Louis, MO) for 4 h, then treated with 1% (v/v) osmium for 2 h, then dehydrated with graded ethanol solutions (30,50,70,90,95, and 100%). Samples were then critical point-dried (Autosamdri-814, Tousimis, Rockville, MD) and gold sputtered (Hummer IV).…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Decellularization method influences early remodeling of an allogenic tissue scaffold

Goktas

Matuska

Pierre

et al. 2013

J. Biomed. Mater. Res.

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Extracellular matrix-based biomaterials are currently pursued as an alternative to autologous transplants for the treatment of gingival recession and periodontal disease. These grafts offer improved tissue regeneration without the need for a second operative procedure used in current treatments to remove nonresorbable synthetic biomaterials. However, while decellularization is necessary to minimize the potential immunological impact, it can significantly modify the materials architectural and biochemical properties. By understanding cellular responses, it is possible to more specifically target varying clinical situations. These investigations assess a novel allogenic scaffold derived from the human umbilical vein and determine the effects of two decellularization approaches (osmotic lysis and the surfactant Triton X-100) on the biological and mechanical properties during early remodeling events. Results show Triton X-100 to be significantly more effective at extracting lipids, while the extraction of the scaffolds bulk protein, GAG and DNA similar between the two treatments. Once seeded, scaffolds prepared with osmotic lysis displayed increased cellular proliferation and reduced metabolic activity compared to scaffolds treated with surfactant. Biomechanical properties were largely preserved and similar between the two treatments. These results suggest that by optimizing scaffold processing conditions, biological events associated with remodeling can be modulated to tailor scaffold function for specific clinical applications.

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“…Apoptosis is a relevant mechanism for chemotherapy-induced cell death in human hematologic malignancies. Previous studies on GS focused on the treatment of osteoarthritis; clinical findings revealed that GS is a safe and effective treatment for alleviating symptoms of osteoarthritis, and GS is now widely used for this purpose in the United States [2,3]. The goal of the present study was to determine the mechanism of GS-induced apoptosis in K562 cells.…”

Section: Introductionmentioning

confidence: 96%

Glucosamine sulfate–induced apoptosis in chronic myelogenous leukemia K562 cells is associated with translocation of cathepsin D and downregulation of Bcl-xL

et al. 2006

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Cathepsin D (cat D) reportedly plays an important role in certain apoptotic processes, the downstream pathways of which involve release of cytochrome c (cyt c) from mitochondria and activation of the caspase cascade. Previous studies revealed that the B-cell lymphoma 2 (Bcl-2) family members Bax or Bid play important roles in apoptotic signal transduction between cat D and mitochondria. Here, we show that glucosamine sulfate (GS) inhibits the proliferation and induces apoptosis of human chronic myelogenous leukemia K562 cells in vitro. GS interfered with the maturation of cat D. Activation of caspase-3, cleavage of poly-(ADP-ribose)-polymerase, release of cyt c, and downregulation of Bcl-xL accompanied GS-induced apoptosis, and these processes were inhibited by the cat D inhibitor pepstatin A. However, we did not detect any altered gene expression of Bcl-2, Bax, or Bid during apoptosis. Translocation of cat D from the lysosome to the cytosol was observed in GS-treated K562 cells. These findings suggest that GS-induced K562 cell apoptosis involves the translocation of cat D from the lysosome to the cytosol. Furthermore, our findings suggest that downregulation of Bcl-xL (but not Bcl-2, Bax, or Bid) connects cat D and the mitochondrial pathway, which causes the release of cyt c and activation of the caspase cascade during GS-induced apoptosis of K562 cells.

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Use of Glucosamine and Chondroitin Sulfatein the Management of Osteoarthritis

Cited by 52 publications

References 18 publications

Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells

Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells

Decellularization method influences early remodeling of an allogenic tissue scaffold

Glucosamine sulfate–induced apoptosis in chronic myelogenous leukemia K562 cells is associated with translocation of cathepsin D and downregulation of Bcl-xL

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