Use of Low-Cost Agro-Industrial Substrate to Obtain Carotenoids from <i>Phaffia rhodozyma</i> in a Bioreactor

Urnau, Letícia; Colet, Rosicler; Reato, Paloma Truccolo; Burkert, Janaína Fernandes de Medeiros; Rodrigues, Eliseu; Gomes, Raul; Jacques, Rosângela Assis; Valduga, Eunice; Steffens, Clarice

doi:10.1089/ind.2018.0027

Cited by 14 publications

(11 citation statements)

References 38 publications

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“…Correlating the fed-batch yield with the optimized single batch obtained by Urnau et al [5] was verified an increase of approximately 140%. Thus, demonstrating that the controlled addition of nutrients (corn steep liquor, rice parboiling water, and crude glycerol) and consequent substrate assimilation resulted in high production of biomass and total carotenoids.…”

Section: Discussionmentioning

confidence: 56%

“…Y-17268 was provided by the Bioprocess Engineering Laboratory, (FURG, RS/Brazil), certified as GRAS (Generally Recognized as Safe). The conditions of inoculum preparation were described previously [5]. This yeast was used for the carotenoid bioproduction in a bioreactor.…”

Section: Conditions Of Cultivation and Carotenoid Bioproductionmentioning

confidence: 99%

“…In the fed-batch bioproduction was employed optimized medium [5] composed of crude glycerol (100 g/L), corn steep liquor (100 g/L), and rice parboiling water (20 g/L). An initial volume of 500 mL of the medium was autoclaved in the Biostat bioreactor (Braun Biotech International) before star the fermentation process.…”

Section: Carotenoid Bioproduction In Fed-batch Bioreactormentioning

confidence: 99%

“…An initial volume of 500 mL of the medium was autoclaved in the Biostat bioreactor (Braun Biotech International) before star the fermentation process. The feed rate for the carotenoid bioproduction employed varied from 50, 75, 112.5 and 150 mL of medium, each 12 h. The operational conditions used were based on our previous work [5]: 1.5 vvm of aeration rate-air with 180 rpm stirring rate, 25C, and 4.0 pH initial for 96 h bioproduction.…”

Section: Carotenoid Bioproduction In Fed-batch Bioreactormentioning

confidence: 99%

“…The natural pigments most studied are the carotenoids, responsible for the yellow, orange, and red colors of some fruits, vegetables, flowers, fish, crustaceous, bacteria, and fungi [1]. The carotenoids production via biotechnology can be obtained by a diversity of microorganisms, such as bacteria [2], yeasts [3][4][5], microalgal [6], and filamentous fungi [7].…”

Section: Introductionmentioning

confidence: 99%

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Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020

Biointerface Res Appl Chem

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This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

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Section: Discussionmentioning

confidence: 56%

Section: Conditions Of Cultivation and Carotenoid Bioproductionmentioning

confidence: 99%

Section: Carotenoid Bioproduction In Fed-batch Bioreactormentioning

confidence: 99%

Section: Carotenoid Bioproduction In Fed-batch Bioreactormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020

Biointerface Res Appl Chem

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Enhancement of astaxanthin production from food waste by Phaffia rhodozyma screened by flow cytometry and feed application potential

Lai

Liu

et al. 2023

Biotech and App Biochem

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Astaxanthin is widely used in food, aquaculture, cosmetics, and pharmaceuticals due to its strong antioxidant activity and coloring ability, but its production from Phaffia rhodozyma remains the main challenge due to the high fermentation cost and low content of carotenoid. In this study, the production of carotenoids from food waste (FW) by a P. rhodozyma mutant was investigated. P. rhodozyma mutant screened by UV mutagenesis and flow cytometry could stably produce high carotenoids at 25°C, with carotenoid production (32.9 mg/L) and content (6.7 mg/g), respectively, increasing by 31.6% and 32.3% compared with 25 mg/L and 5.1 mg/g of wild strain. Interestingly, the carotenoid production reached 192.6 mg/L by feeding wet FW, which was 21% higher than batch culture. The 373 g vacuum freeze‐dried products were obtained from the fermentation of 1 kg FW by P. rhodozyma, which contained 784 mg carotenoids and 111 mg astaxanthin. The protein, total amino acids, and essential amino acids content of the fermentation products were 36.6%, 40.5%, and 18.2% (w/w), respectively, and lysine‐added fermentation products had the potential of high‐quality protein feed source. This study provides insights for the high‐throughput screening of mutants, astaxanthin production, and the development of the feed potential of FW.

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Influence of redox potential on the accumulation of poly(3-hydroxybutyrate) by Bacillus megaterium

Hassemer

Nascimento

Lin

et al. 2023

Bioprocess Biosyst Eng

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Use of Low-Cost Agro-Industrial Substrate to Obtain Carotenoids from Phaffia rhodozyma in a Bioreactor

Cited by 14 publications

References 38 publications

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

Enhancement of astaxanthin production from food waste by Phaffia rhodozyma screened by flow cytometry and feed application potential

Influence of redox potential on the accumulation of poly(3-hydroxybutyrate) by Bacillus megaterium

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