Use of molecular markers of potato golden nematode resistance genes H1 and GRO1

Biryukova, V. A.; Журавлев, А. А.; Abrosimova, S. B.; Костина, Л. И.; Khromova, L. M.; Shmyglya, I. V.; Morozova, Nadya; Kirsanova, S. N.

doi:10.3103/s1068367408060013

Cited by 22 publications

(17 citation statements)

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“…However some markers were useful for detection of resistant cultivars or clones. The marker TG689 linked to the gene H1 is the most common in resistant cultivars (Biryukova et al 2008). Marker TG689 was already used in the breeding programme, as in case of selection of the cultivar Missaukee (Douches et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays the most widely used are CAPS and SCAR markers (Barone 2004). The following markers are recommended as a good tool for selection genotypes resistant to PCN: U14 and X02 (Jacobs et al 1996), CP113 (Niewöhner et al 1995, Skupinová et al 2002, TG689 (Sagredo et al 2004;Biryukova et al 2008), Gro 1,4 (Paal et al 2004;Gebhardt et al 2006), 77R (van der Voort et al 1999, SPUD1636 (Bryan et al 2002) and SNP marker named HC (Sattarzadeh et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

2011

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Globodera rostochiensis and Globodera pallida are parasitic root cyst nematodes of potato (PCN), which significantly reduce yield and quality. The genetics and available molecular markers should make resistance to nematodes an excellent candidate for marker assisted selection. The study presents results of testing the suitability of known molecular markers for detection of resistance in a set of cultivars. We revealed some inconsistencies in genealogical data of the cultivars and showed inconsistent usefulness in detecting resistant cultivars. The marker TG689 was identified in almost all cultivars resistant to G. rostochiensis and together with other markers was used for verifying the resistance in a group of breeding lines. The marker TG689 was the most effective. However, the efficiency of tested markers in selecting resistant individuals might be inadequate. In the case of quantitative resistance to G. pallida, marker SPUD1636 was found in resistant cultivars, and not in susceptible cultivars, but turned out to be inefficient in selecting resistant breeding lines.Resumen Globodera rostochiensis y Globodera pallida son nematodos de quiste parásitos de la raíz de la papa (PCN) que reducen significativamente el rendimiento y la calidad. La genética y los marcadores moleculares disponibles, deberían de hacer de la resistencia a nematodos un excelente candidato para selección asistida con marcadores. El estudio presenta resultados de probar lo deseable de los marcadores moleculares conocidos para la detección de resistencia en un grupo de variedades. Revelamos algunas inconsistencias en datos genealógicos de las variedades y mostramos uso inconsistente en la detección de variedades resistentes. El marcador TG689 se identificó en casi todas las variedades resistentes a G. rostochiensis y junto con otros marcadores se usó para verificar la resistencia en un grupo de líneas de mejoramiento. El marcador TG689 fue el más efectivo. No obstante, podría ser inadecuada la eficiencia de los marcadores probados en la selección de individuos resistentes. En el caso de resistencia cuantitativa a G. pallida, se encontró el marcador SPUD1636 en variedades resistentes y no en los susceptibles, pero resultó ser ineficiente en la selección de líneas de mejoramiento resistentes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

2011

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“…DNA marker Gro1-4 can detect Gro1-4 (Biryukova et al 2008, Gebhardt et al 2006). Gro1-4 is a member of the Gro1 locus, which confers nearly absolute resistance to all pathotypes of G. rostochiensis , and is therefore considered a useful resistance gene (Barone et al 1990, Paal et al 2004).…”

Section: Introductionmentioning

confidence: 99%

DNA marker-assisted evaluation of potato genotypes for potential resistance to potato cyst nematode pathotypes not yet invading into Japan

et al. 2012

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One of major objectives of crop breeding is conferring resistance to diseases and pests. However, large-scale phenotypic evaluation for many diseases and pests is difficult because strict controls are required to prevent their spread. Detection of disease resistance genes by using DNA markers may be an alternative approach to select potentially resistant accessions. Potato (Solanum tuberosum L.) breeders in Japan extensively use resistance gene H1, which confers nearly absolute resistance to potato cyst nematode (Globodera rostochiensis) pathotype Ro1, the only pathotype found in Japan. However, considering the possibility of accidental introduction of the other pathotypes, breeding of resistant varieties is an important strategy to prevent infestation by non-invading pathotypes in Japan. In this study, to evaluate the prevalence of resistance genes in Japanese genetic resources, we developed a multiplex PCR method that simultaneously detects 3 resistance genes, H1, Gpa2 and Gro1-4. We revealed that many Japanese varieties possess not only H1 but Gpa2, which are potentially resistant to other pathotypes of potato cyst nematode. On the other hand, no genotype was found to have the Gro1-4, indicating importance of introduction of varieties having Gro1-4. Our results demonstrate the applicability of DNA-marker assisted evaluation of resistant potato genotypes without phenotypic evaluation.

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“…The TG689 marker has to date been the marker of choice for the prediction of PCN resistance. However, TG689 is pedigree‐specific, exhibiting loss of allelic association with the H1 resistance gene in several cultivars (Biryukova et al. 2008, Schultz et al.…”

Section: Discussionmentioning

confidence: 99%

Evaluation and implementation of a potential diagnostic molecular marker for H1‐conferred potato cyst nematode resistance in potato (Solanum tuberosum L.)

et al. 2012

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Potato cyst nematode (PCN) is a major pest of potato crops. Markerassisted selection of PCN-resistant cultivars is consequently a key objective of many potato breeding programmes. The genetic marker TG689, linked to the resistance gene H1, is the current marker of choice for the prediction of PCN resistance. Polymorphism at TG689 was evaluated across 373 cultivars from Australian and Scottish breeding programmes, revealing the loss of association between the predictive allele and trait locus. Two recently identified genetic markers flanking the H1 locus were then evaluated for association with PCNresistant phenotypes across 73 cultivars. With a single exception, presence or absence of a 452-bp amplicon for the 57R marker was predictive for the target phenotype. Further evaluation of the 57R marker on more than 300 cultivars from the Australian and Scottish potato breeding programmes provided additional evidence for robust diagnostic capability. To enable routine implementation, the 57R marker was converted into a high-throughput, fluorochrome detection-based assay and screened across a broader collection of parental cultivars and advanced breeding selections for the prediction of PCN resistance.

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Use of molecular markers of potato golden nematode resistance genes H1 and GRO1

Cited by 22 publications

References 3 publications

Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp

DNA marker-assisted evaluation of potato genotypes for potential resistance to potato cyst nematode pathotypes not yet invading into Japan

Evaluation and implementation of a potential diagnostic molecular marker for H1‐conferred potato cyst nematode resistance in potato (Solanum tuberosum L.)

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