Use of molecular methods to characterizeMoraxella catarrhalis strains in a suspected outbreak of nosocomial infection

Morgan, M. G.; McKenzie, Hamish; Enright, Mark C.; Bain, M.; Emmanuel, F.X.S.

doi:10.1007/bf01962069

Cited by 33 publications

(26 citation statements)

References 32 publications

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“…Six M. catarrhalis isolates from a cluster of infections involving five patients in a respiratory unit were shown to be identical to each other and different from other, unrelated strains from the same institution (200). Both methods provided good discrimination between strains, but they were not always in complete agreement (166,200). Thus, the use of more than one typing technique was recommended.…”

Section: Nosocomial Infectionsmentioning

confidence: 99%

Moraxella catarrhalis: from Emerging to Established Pathogen

et al. 2002

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SUMMARY Moraxella catarrhalis (formerly known as Branhamella catarrhalis) has emerged as a significant bacterial pathogen of humans over the past two decades. During this period, microbiological and molecular diagnostic techniques have been developed and improved for M. catarrhalis, allowing the adequate determination and taxonomic positioning of this pathogen. Over the same period, studies have revealed its involvement in respiratory (e.g., sinusitis, otitis media, bronchitis, and pneumonia) and ocular infections in children and in laryngitis, bronchitis, and pneumonia in adults. The development of (molecular) epidemiological tools has enabled the national and international distribution of M. catarrhalis strains to be established, and has allowed the monitoring of nosocomial infections and the dynamics of carriage. Indeed, such monitoring has revealed an increasing number of Β-lactamase-positive M. catarrhalis isolates (now well above 90%), underscoring the pathogenic potential of this organism. Although a number of putative M. catarrhalis virulence factors have been identified and described in detail, their relationship to actual bacterial adhesion, invasion, complement resistance, etc. (and ultimately their role in infection and immunity), has been established in a only few cases. In the past 10 years, various animal models for the study of M. catarrhalis pathogenicity have been described, although not all of these models are equally suitable for the study of human infection. Techniques involving the molecular manipulation of M. catarrhalis genes and antigens are also advancing our knowledge of the host response to and pathogenesis of this bacterial species in humans, as well as providing insights into possible vaccine candidates. This review aims to outline our current knowledge of M. catarrhalis, an organism that has evolved from an emerging to a well-established human pathogen.

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Section: Nosocomial Infectionsmentioning

confidence: 99%

Moraxella catarrhalis: from Emerging to Established Pathogen

et al. 2002

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“…Pre vi ous meth ods used to type M catar rhalis have re lied upon phe no typic char ac ter is tics. However, these meth ods lack re pro duci bil ity be cause of the un pre dict able ex pres sion of the char ac ter is tic be ing evaluated (9,15). Mo lecu lar tech niques have been more suc cessful.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Typing of Moraxella Catarrhalis by Pulsed Field Gel Electrophoresis

Davison

Low

Cruz³

et al. 1995

Canadian Journal of Infectious Diseases and Medical Microbiolog

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SM DAVI SON, DE LOW, RH CRUZ, D BEAU LIEU, SR SCRIVER. Epi de mi ol ogi cal typ ing ofMo rax ella ca tar rhalis by pulsed field gel elec tro pho re sis. Can J In fect Dis 1995;6(3):141-144. Pulsed field gel elec tro pho re sis (PFGE) was used to com pare 59 strains of Mo rax ella ca tar rhalis to evalu ate PFGE for the epi de mi ol ogi cal typ ing of this or gan ism. PFGE-ge ne rated pat terns were com pared with those ob tained by small frag ment re stric tion en zyme analy sis (REA) and species-specific probe hy bridi za tion. The strains used in the study were iso lated from vari ous geo graphic lo ca tions and in cluded proven epi de mi ol ogi cally re lated strains. PFGE yielded more unique pat terns than DNA-DNA hy bridi za tion -30 ver sus 18, re spec tively -but fewer than REA, which gen er ated 45 unique pat terns. Strains that dem on strated the same REA pat tern or DNA-DNA hy bridi za tion pat tern did not al ways dem on strate the same PFGE pat tern. For ex am ple, in 23 epide mi ol ogi cally un re lated strains that shared six REA pat terns, PFGE dif fer en ti ated the iso lates into 12 pat terns. Conversely, strains that dem on strated the same PFGE pat tern did not al ways dem on strate the same REA pat tern or hy bridi za tion pat tern. For ex am ple, in 42 strains that shared 13 PFGE pat terns, REA dif fer en ti ated the iso lates into 31 patterns and DNA-DNA hy bridi za tion dif fer en ti ated them into 16 pat terns. How ever, com pared with REA, PFGE yielded less com plex pat terns that were more eas ily com pa ra ble, and com pared with DNA-DNA hy bridi za tion, PFGE was tech ni cally eas ier. Key Words: Gene prob ing, Mo rax ella ca tar rhalis, Pulsed field gel elec tro pho re sis, Re stric tion en zyme analy sisTypage épidémiologique de Moraxella catarrhalis par électrophorèse en champ pulsé RÉS UMÉ : L'é lec tro phorèse en champ pulsé (ECP) a été util isée afin de com parer 59 souches de Mo rax ella ca tar rhalis en vue de dé ter mi ner si l'ECP pou vait être util isée pour le typage épidémi olo gique de cet or gan isme pa thogène. Les modèles gé né rés par ECP ont été com parés à ceux qu'a vaient donné une ana lyse des en zymes de re stric tion de pe tits frag ments (AER) et une hy bri da tion par sonde spé ci fique à l'e spèce. Les souches util isées dans l'étude ont été isolées à par tir de diffé rents sites géo graphiques et com prenaient des souches épidémi olo gique ment ap par entées. L'ECP a gé -néré des modèles plus uniques que l'hy bri da tion DNA-DNA, soit 30 con tre 18, re spec tive ment, mais moins que l'AER, qui a gé néré 45 modèles uniques. Les souches dont le modèle était sem bla ble à l'AER ne se sont pas tou jours ex primé sembla ble ment à l'ECP. Par ex em ple, des 23 souches indépen dan tes sur le plan épidémi olo gique mani fes tant six modèles voir page suivante CAN

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“…These observations suggest that the presence of a susceptible population contributed to the clusters. Preliminary observations involving typing of strains indicate that some of the clusters involved infections with several distinct strains of B. catarrhalis (13,121). Other studies implicate person-to-person transmission and suggest that hospital personnel may be involved in transmission (135,144).…”

Section: Nosocomial Infectionmentioning

confidence: 99%

“…The most widely used method to type strains of B. catarrhalis to date has been restriction endonuclease analysis of genomic DNA (44,45,58,89,118,121,134,135,144). More recently, a more refined form of restriction endonuclease analysis involving pulsed-field gel electrophoresis of large fragments of genomic DNA has been developed (99,102).…”

Section: Genotype-based Typing Systemsmentioning

confidence: 99%

Lower respiratory tract infection

Sharland¹

2016

OSH Manual of Childhood Infections

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Use of molecular methods to characterizeMoraxella catarrhalis strains in a suspected outbreak of nosocomial infection

Cited by 33 publications

References 32 publications

Moraxella catarrhalis: from Emerging to Established Pathogen

Moraxella catarrhalis: from Emerging to Established Pathogen

Epidemiological Typing of Moraxella Catarrhalis by Pulsed Field Gel Electrophoresis

Lower respiratory tract infection

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