Use of novel picolinoyl derivatization for simultaneous quantification of six corticosteroids by liquid chromatography-electrospray ionization tandem mass spectrometry

Derivatization of Secondary Aliphatic Alcohols to Picolinates – A New Option for HPLC Analysis with Chiral Stationary Phase

“…On the other hands, several hydroxy steroids have been changed to picolinates for LC-ESI-MS spectroscopy. [10][11][12] However, these studies have aimed to develop high sensitivity method, which was different from our study.…”

Possible Contribution of 2-Aminoethoxydiphenyl-borate-sensitive Ca2+ Mobilization to Adrenocorticotropin-induced Glucocorticoid Synthesis in Rat Adrenocortical Cells

“…only AZFR cells were used for this study and were separated from the zona glomerulosa by peeling the adrenal gland. After isolation by dispersion in HEPEs dissociation buffer (137 mM naCl, 5 mM KCl, 0.7 2APB-sEnsITIVE [Ca 2+ ] i MoBILIZATIon In ACTH-InduCEd sTERoIdogEnEsIs Co Ltd. (Kawasaki, Japan) [16]. To determine the effects of the soCE inhibitor on steroidogenic enzymes, we considered the concentration of pregnenolone to indicate P450 scc activity, the ratio of progesterone to pregnenolone as 3β-hydroxysteroid dehydrogenase (3β-HSD) activity and the ratio of 11-DOC to progesterone for P450 c11 .…”

Preparation and Structural Elucidation of the Picolinyl Ester of Aldosterone for Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

“…[11][12][13][14][15][16][17][18] In our previous studies, we have demonstrated that the picolinyl derivatization of hydroxyl group in the steroid molecules proceeded smoothly and quantitatively, and the picolinyl derivative thus obtained by simple one-step procedure exhibited preferable mass spectral advantages in enhancing sensitivity to LC-ESI-MS/MS. [19][20][21][22] The wide application of picolinyl derivatization to steroid hydroxy group irrespective of positional and/or steric factors is another great advantage of this reagent 19) when compared to recently developed reagents. During the course of our studies, we attempted to develop a sensitive method to quantify biologically important and structurally complicated aldosterone based on the use of a picolinyl derivatization.…”

“…[19][20][21][22] The reaction mixture was diluted with 5% NaHCO 3 (2 ml) and the resulting mixture was transferred onto Bond Elut C18 cartridge (60 mgϫ2, pre-conditioned with MeOH 3 ml and H 2 O 3 ml) by two portions. After washing the cartridges subsequently with H 2 O (3 ml), 5% HCl (3 ml), H 2 O (3 ml) and MeCN-H 2 O (20 : 80, v/v, 3 ml), the products were eluted with MeCN-H 2 O (80 : 20, v/v, 3 ml).…”