Use of optical imaging to progress novel therapeutics to the clinic

Byrne, William L.; DeLille, Alexandra; Kuo, Chaincy; Jong, Johannes S. de; Dam, Gooitzen M. van; Francis, Kevin P.; Tangney, Mark

doi:10.1016/j.jconrel.2013.05.004

Cited by 28 publications

(14 citation statements)

References 142 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increasing number of studies have demonstrated the optical imaging as a modality for preclinical in vivo examination of tumor detection, growth, and response to different treatments 26 27 28 29 30 . Because of its efficiency, the clinical potential for the in vivo optical imaging is becoming apparent 31 32 . Compared to the previously reported models using cells single-labeled with luciferase 6 7 , our model demonstrates the advanced evaluation of tumor characterization using ex vivo optical imaging with fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Imaging of tumor clones with differential liver colonization

Oshima

Wightman²,

Uppal³

et al. 2015

Sci Rep

View full text Add to dashboard Cite

We present a model of hepatic colorectal metastases which represents monoclonal cell lines double-labeled by luciferase and tdTomato. These cells form liver metastasis in varying numbers and patterns similar to those observed in patients. Using in vivo and ex vivo luminescent and fluorescent imaging we determine the growth kinetics and clonogenic frequency of tumor cells colonizing liver. Molecular profiling detected stable expressional differences between clones consistent with their phenotypes. The data indicate that clinically relevant phenotypes of liver metastases can be modeled in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Imaging of tumor clones with differential liver colonization

Oshima

Wightman²,

Uppal³

et al. 2015

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Fluorescent dye-based guidance during surgical interventions is being recognised as an improvement in the accuracy of clinical care [1][2][3]. In clinical trials, fluorescence imaging has been used as a sole modality or in a bimodal/hybrid form, wherein it extends the field of nuclear medicine [4].…”

Section: Introductionmentioning

confidence: 99%

The influence of systematic structure alterations on the photophysical properties and conjugation characteristics of asymmetric cyanine 5 dyes

et al. 2018

View full text Add to dashboard Cite

The light spectrum above 650 nm allows for good tissue penetration depths, far-red and nearinfrared fluorescent dyes are therefore popular fluorophores applied in (bio)medical diagnostics, including image-guided surgery. However, near-infrared fluorescent dyes often suffer from instability and limited brightness, two important features that, together with the labelling efficiency (e.g., non-one-or di-conjugated products) and serum-dye interactions are key elements that drive in vivo characteristics. Due to the fact that stability and brightness of far-red fluorophores are often superior over near-infrared dyes, interest in the use of dyes such as Cy5 is increasing. As there are clear indications that the influence of the chemical structure on the (photo)physical properties of a dye is dye-structure-dependent, the (photo)physical properties of ten structural variants of asymmetrical Cy5-(R1)R2-(R3)COOH (R representing the varied substituents) were extensively studied, While stacking in solution was not induced in most of the Cy5 far-red fluorophores, multimers and 2 stacking characteristics were observed in protein conjugates. And although all dye variants were shown to be stable towards photobleaching, clear differences in brightness and serum interactions were found. Combined, these findings indicate that the chemical substituents prominently influence the photophysical properties of Cy5 dyes, a feature that should be considered when using fluorescent dyes in future tracer development.

show abstract

“…In the basic science and preclinical settings, these techniques have delivered tremendous advances in understanding disease biology (1). They also have considerable utility in noninvasive evaluation of efficacy for drug development (2,3). The capability of such technologies to detect pathologic molecular processes, particularly to identify malignancy, has been a major driving force in their development.…”

mentioning

confidence: 99%

Clinical Cerenkov Luminescence Imaging of¹⁸F-FDG

2013

View full text Add to dashboard Cite

The aim of this study was to determine the feasibility of Cerenkov luminescence (CL) imaging of patients undergoing diagnostic 18F-FDG scans to detect nodal disease. Methods Patients undergoing routine 18F-FDG PET/CT for various malignancies consented to being scanned for CL. White-light and Cerenkov images (5-min acquisition) of the surface of the patient contralateral to and at the site of nodal 18F-FDG uptake were acquired using a cooled, intensified charge-coupled-device camera. Results The camera demonstrated linear correlation between activity and counts into the low nanocurie range using 18F-FDG. Imaging of patients revealed the presence of 18F-FDG uptake in nodes that demonstrated uptake on PET. A correlation between maximum standardized uptake value from PET and counting rate per area on the CL imaging was established. Conclusion CL imaging with diagnostic doses of 18F-FDG is feasible and can aid in detecting disease in the clinical setting.

show abstract

Use of optical imaging to progress novel therapeutics to the clinic

Cited by 28 publications

References 142 publications

Imaging of tumor clones with differential liver colonization

Imaging of tumor clones with differential liver colonization

The influence of systematic structure alterations on the photophysical properties and conjugation characteristics of asymmetric cyanine 5 dyes

Clinical Cerenkov Luminescence Imaging of¹⁸F-FDG

Contact Info

Product

Resources

About

Use of optical imaging to progress novel therapeutics to the clinic

Cited by 28 publications

References 142 publications

Imaging of tumor clones with differential liver colonization

Imaging of tumor clones with differential liver colonization

The influence of systematic structure alterations on the photophysical properties and conjugation characteristics of asymmetric cyanine 5 dyes

Clinical Cerenkov Luminescence Imaging of18F-FDG

Contact Info

Product

Resources

About

Clinical Cerenkov Luminescence Imaging of¹⁸F-FDG