Use of phytase at graded levels for improving nutrient digestibility, growth and hematology of Catla catla fingerlings fed Moringa oleifera seed meal (MOSM) based diet

Abstract

Present research work was conducted to evaluate the effect of phytase supplementation on nutrient digestibility, growth performance and hematological indices of Catla catla (Hamilton, 1822) fingerlings fed Moringa oleifera seed meal (MOSM) based diet. Existence of phytate in plant byproducts decreases the bioavailability of nutrients to fish, resulting in low nutrients availability, poor fish growth and hematological indices in fish body. Moringa byproducts such as M. oleifera seed meal (MOSM) was used as test ingredient to formulate experimental diet. Experimental diet was divided into six test diets and were supplemented with graded levels (0, 300, 600, 900, 1200 and 1500 FTU kg -1 ) of phytase. The fingerlings were fed at the rate of 4% of live wet weight twice a day in V-shaped indoor tanks. Phytase supplementation showed significant (p<0.05) improvement in bioavailability of nutrients, growth indices and hematological parameters. Results showed that maximum growth performance, digestibility of nutrients and improved hematological values of C. catla fingerlings were noted at 900 FTU kg -1 level of phytase supplementation in MOSM based test diet. It was concluded that phytase supplementation at 900 FTU kg -1 level was helpful to develop an eco-friendly and cost effective fish feed incorparating moringa seed meal.

Effectiveness of phytase pre-treatment on growth performance, nutrient digestibility and mineral status of common carp (Cyprinus carpio) juveniles fed Moringa by-product based diet

“…ADC of GE also increased to its maximum (71%) at 950 FTU kg −1 amount of PHY, followed by digestibility co-efficient (69%) at 800 FTU kg −1 . Hussain et al (2017) reported maximum GE (74%) at 900 FTU kg −1 in C. catla , that is very close to our results. While, lowest value of GE (53%) was found at diet with 0 FTU kg −1 .…”

“…Maximum growth performance in terms of WG (19 g), FCR (1.10) and SGR (1.67) was estimated in C. carpio at 950 FTU kg −1 of PHY, followed by 800 FTU kg −1 . Like above mentioned results, Hussain et al, 2017 , Shahzad et al, 2018b found highest growth performance in C. catla when fed on a PHY supplemented (900 FTU kg −1 ) moringa by-product based diet in comparison of control and other PHY supplemented diets. Maas et al (2019) observed improved growth performance in Nile tilapia at 1000 FTU kg −1 supplemented in sunflower meal based diet.…”

Role of microbial phytases in improving fish health

“…The involvement of phytase in increasing protein digestibility is not direct, in fact, it is an outcome of phytase action on the digestibility of amino acid 48 . Phytase helps in retaining more nutrients inside the fish body 32,70,71,131 . Increased digestibility of minerals such as Fe, Mg, Ca, P, K, Zn, Cu, Na, Al, Mn, Sr, Cr and Pb has been observed in O .…”

“…48 Phytase helps in retaining more nutrients inside the fish body. 32,70,71,131 Protein as well as P digestibility was higher when dietary protein and phytase were added to the diet of O. mykiss 60 and C. gariepinus. 119 Although, the apparent digestibility of protein, ash, and P was more in P. maxima, the absorption of minerals remained unaffected.…”

Effects of phytase inclusion in diets containing rice protein concentrate (RPC) on the nutrient digestibility, growth and chemical characteristics of rohu (Labeo rohita)

“…Due to this potential, the utilization of phytase is currently being extensively assessed [15]. Various studies have been conducted on phytase supplementation in plant-based diets, which have shown enhanced growth performance and nutrient utilization in fish species [16][17][18][19]. However, no study on RPC-based diets has been reported.…”