Use of PIXE/PIGE for sequential Ca and F measurements in root carious model

Yagi, K.; Yamamoto, Hiroko; Uemura, Reo; Matsuda, Yasuhiro; Okuyama, Katsushi; Ishimoto, Takuya; Nakano, Takayoshi; Hayashi, Mikako

doi:10.1038/s41598-017-14041-4

Cited by 11 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Teeth were collected after written patient informed consent, under protocols approved by the Research Ethics Committee. Specimen preparations and measurements of the calcium and fluoride distributions and concentrations before and after demineralization were performed according to previously reported methods 17) .…”

Section: Methodsmentioning

confidence: 99%

“…The calcium and fluoride distributions and concentrations in specimens were measured using the PIXE/PIGE system at the Wakasa Wan Energy Research Center according to a previous report 17) . A 2.5 MeV proton beam was used to bombard specimens in normal atmospheric conditions.…”

Section: Measurements Of Calcium and Fluoride Distributions And Concentrations Before Demineralizationmentioning

confidence: 99%

“…2C). On the superimposed image, the calcium loss (wt%•µm) and the fluoride uptake (ppmF•µm) was calculated according to previously reported methods 17) .…”

Section: Comparisons Of Calcium and Fluoride Distributions Before And After Demineralizationmentioning

confidence: 99%

“…In a previous study 17) , we successfully established a multi-elemental sequential measuring method using an in-air micro-proton-induced X-ray and gamma-ray emission (PIXE/PIGE) system to identify the distribution and concentration of calcium and fluoride in human dentin models. We found that PIXE/PIGE potentially offers a useful and advantageous technique for studying caries development in combination with conventional techniques such as TMR and µCT.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In-air micro-proton-induced X-ray/gamma-ray emission analysis of the acid resistance of root dentin after applying fluoride-containing materials incorporating calcium

et al. 2021

View full text Add to dashboard Cite

This study employed an in-air micro-proton-induced X-ray/gamma-ray emission system to assess the effectiveness of fluoridecontaining materials (FCMs) incorporating calcium in preventing root caries. Dentin surfaces of human third molars were coated with one of three FCMs: fluoride-releasing glass-ionomer cement (F7) and experimental materials in which half (P1) or all (P2) of the strontium in F7 was replaced with calcium. Dentin without FCM coating served as the control. Specimens were immersed in saline at 37°C for 1 month, sectioned, and then demineralized. Calcium loss after demineralization was lower in the Ca-substituted groups than in the Ca-unsubstituted groups (p<0.05). Calcium loss was negatively correlated with fluoride uptake (p<0.01). In the F7, P1, and P2 groups, the retraction of the dentin surface was significantly suppressed as compared with the control group. FCMs incorporating calcium improved the acid resistance of root dentin and could help prevent root caries.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Measurements Of Calcium and Fluoride Distributions And Concentrations Before Demineralizationmentioning

confidence: 99%

“…2C). On the superimposed image, the calcium loss (wt%•µm) and the fluoride uptake (ppmF•µm) was calculated according to previously reported methods 17) .…”

Section: Comparisons Of Calcium and Fluoride Distributions Before And After Demineralizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In-air micro-proton-induced X-ray/gamma-ray emission analysis of the acid resistance of root dentin after applying fluoride-containing materials incorporating calcium

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Dentin specimens were prepared as previously reported (Fig. 2A) 44 . Root dentin specimens were sectioned perpendicularly to the axis, at points 0.5 mm above and 7.0 mm below the cement-enamel junction (CEJ).…”

Section: Methodsmentioning

confidence: 99%

UVA-activated riboflavin promotes collagen crosslinking to prevent root caries

Uemura

Miura

Ishimoto

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Root caries is an increasingly problem in aging societies with severe implications for the general health and wellbeing of large numbers of people. Strengthening type-I collagen, a major organic component of human dentin, has proved effective in preventing root caries. This study sought to determine whether exposure to riboflavin followed by UVA irradiation (RF/UVA) could promote additional collagen crosslinking, and thus improve the acid and enzymatic resistance of human dentin under simulated oral environments. If so, it could offer potential for treatment of the intractable problem of root caries. The greatest flexural strengths were found in dentin exposed to a 0.1% riboflavin solution for 1 minute followed by 1,600 mW/cm2 UVA irradiation for 10 minutes. Mineral loss and lesion depth were significantly lower in the RF/UVA group than in the control group. The microstructures of dentinal tubules and collagen networks after RF/UVA treatment retained their original forms after acidic and enzymatic degradation. In conclusion, RF/UVA treatment may be a new method for preventing root caries with promising prospects for clinical application.

show abstract