Use of specific sugars to inhibit bacterial adherence to equine endometrium in vitro

King, S.S.; Young, Deena A.; Nequin, Lynn G.; Carnevale, E.M.

doi:10.2460/ajvr.2000.61.446

Cited by 34 publications

(17 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We reviewed the published literature and found that there was strong evidence for a positive link between adherence, the presence of fimbriae, and mannosebinding activity by bacteria (Harper et al, 1978;Ofek and Beachey, 1978;Sharon, 1987Sharon, , 2006. For several (Stepinska and Trafny, 1995;King et al, 2000;McEwan et al, 2008). Earlier studies have shown that at least three different types of lectin-like proteins are responsible for the attachment of the saccharide structures of epithelial cells to the adherence proteins of gram-negative bacteria, which are mediated in a mannose-sensitive manner.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner

Liu

Hou

Zhu³

et al. 2010

Oncogene

View full text Add to dashboard Cite

To identify more therapeutic targets and clarify the detailed mechanisms of Pseudomonas aeruginosa-mannosesensitive hemagglutinin (PA-MSHA) on breast cancer cells both in vitro and in vivo. PA-MSHA was administered to epidermal growth factor receptor (EGFR)-positive human breast cancer cell lines MDA-MB-231HM and MDA-MB-468 in vitro and to mice bearing tumor xenografts. The mannose cocultured test was used to detect the effect of mannose on PA-MSHA-induced cell proliferation, cell cycle arrest, apoptosis, and EGFR pathway signaling. We found that cells stimulated with PA-MSHA exhibited a downregulation of EGFR signaling. The addition of mannose partially inhibited the PA-MSHA-stimulated cell anti-proliferative effect, cell apoptosis, cell cycle arrest, activation of apoptosis-associated caspases, and even downregulation of the EGFR signaling pathway. In vivo, PA-MSHA treatment significantly suppressed mammary tumorigenesis in xenografts in mice and decreased lung metastasis in MDA-MB-231HM celltransplanted mice. Tumor sample analyses confirmed inhibition of the EGFR pathway in the PA-MSHAtreated mice. In conclusion, this study showed that the involvement of the mannose-mediated EGFR pathway has a critical function in the preclinical rationale for the development of PA-MSHA for the treatment of human breast cancer. It also suggests the potentially beneficial use of PA-MSHA in adjuvant therapy for breast tumors with EGFR overexpression.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner

Liu

Hou

Zhu³

et al. 2010

Oncogene

View full text Add to dashboard Cite

show abstract

“…Almost all isolates of E. coli , as well as other members of the Enterobactericeae, such as Enterobacter, Klebsiella, Shigella , and Salmonella , attach to mannose receptors by means of type 1 fimbriae 2–6. Other bacteria also possess mannose‐specific adhesins, but other than type 1 fimbriae, these include Pseudomonas, Vibrio cholerae, Lactobacillus, Streptococcus zooepidemicus , the plant pathogen Ralstonia solanacearum , and the cyanobacterium Microcystis aeruginosa 7–12…”

Section: Introductionmentioning

confidence: 99%

Food and feed components for gut health‐promoting adhesion of E. coli and Salmonella enterica

Becker

Galletti

2008

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND: A host runs less risk of contracting a gastrointestinal infection when enteropathogenic bacteria adhere to dietary fibers instead of to epithelial cell receptors. The aim of this study was to test the binding capacity of food and feed components for intestinal bacteria from various hosts using a miniaturized in vitro assay. In total, 18 dietary components were tested with four strains of E. coli, seven strains of Salmonella enterica and two strains of Lactobacillus.

show abstract

“…The glycoprotein secretions of uterine glands and the carbohydrates of the glycocalyx are important markers for differentiation and functionality. Glycoconjugates are known to promote bacterial attachment to the endometrial cell surface (King et al 2000), which is of importance in the emergence of pyometra, if the invasion of the uterus with Escherichia coli is leading to metritis (Johnston et al 2001). Therefore, any alterations of the glycocalyx on the epithelial surface are noteworthy.…”

Section: Discussionmentioning

confidence: 99%

A three-dimensional culture model of canine uterine glands

Stadler

Handler

Schoenkypl³

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

The canine endometrium is frequently affected by severe alterations with unclear pathogenesis and is, therefore, an important subject of research in veterinary gynecology. Therefore, the aim of our study was to establish a three-dimensional in vitro system of the canine endometrium suitable for experimental approaches. For this reason, intact uterine glands were isolated from canine uteri and placed together with stromal cells on culture dishes coated with several extracellular matrix components (collagen I, IV, fibronectin, laminin, gelatin, Matrigel) for up to 4 d to support differentiation of cultured cells. Immunohistochemical detection of laminin on freshly isolated glands showed a partial preservation of the basement membrane--an important factor for epithelial differentiation. Glandular structures were differentiated and polarized during culture time as shown by electron microscopy. Signs of degeneration and loss of cell-cell adhesions as seen occasionally on day 4 depended on the individual dog. In general, morphology was best preserved on Matrigel matrix. No significant changes of cultured glandular explants were observed concerning proliferation and steroid receptor (estrogen, progesterone) expression when compared with the original uterine tissue as assessed by immunohistochemical staining. Lectin histochemistry revealed comparable results for the in vivo endometrial glands and the cultured glandular explants during the whole culture period. This in vitro reconstitution of the canine endometrium is a promising tool to study the cyclic events in the normal endometrium as well as alterations in the affected uterus.

show abstract

Use of specific sugars to inhibit bacterial adherence to equine endometrium in vitro

Cited by 34 publications

References 22 publications

Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner

Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner

Food and feed components for gut health‐promoting adhesion of E. coli and Salmonella enterica

A three-dimensional culture model of canine uterine glands

Contact Info

Product

Resources

About