Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

Androgen action on hepatic vitellogenin synthesis in the eel, Anguilla japonica is suppressed by an androgen receptor antagonist

“…Some recent studies on androgen actions are also in favor of biological significance of androgen and AR in fish liver. A subtractive hybridization and cDNA array analysis of male fish liver treated with DHT revealed that Vg gene was the most upregulated gene amongst the identified up-regulated genes by the androgen [23]. Furthermore, 11-ketotestoterone, a nonaromatizable male specific androgen, treated to female eels did show positive effects on liver growth and oocyte development [24].…”

Functional Genomics of Stress Responses in Fish

“…A reviews in fisheries science vol. 16 S1 2008 similar approach was also employed by Blum et al (2004) to identify androgen-related effects in largemouth bass (Micropterus salmoides). Association between gene expression analysis and a standardized embryo toxicity assay using zebrafish (DarT test) has been used to test effects of 3,4-dichloroaniline toxicity (Voelker et al, 2007).…”

Application of SSH and a macroarray to investigate altered gene expression in Mytilus edulis in response to exposure to benzo[a]pyrene

“…Until recently the lack of genomic data available for mussel (Venier et al, 2003) has confounded development of novel biomarkers at the nucleic acid level. However, discovery of novel biomarkers using suppression subtractive hybridisation (SSH) has been shown to be highly useful in aquatic vertebrates (Blum et al, 2004;Brown et al, 2004a;Brown et al, 2004b;Sheader et al, 2004) where no genomic data are available. The usefulness of this methodology in aquatic invertebrates was assessed using SSH and a nylon macroarray to investigate altered gene expression in digestive gland of Mytilus edulis in response to exposure to benzo[a]pyrene (BaP).…”