Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs

Lima, Víctor Fernando Santana; Ramos, Rafael Antonio Nascimento; Lepold, Raphael; Cringoli, Giuseppe; Rinaldi, Laura; Faustino, Maria Aparecida da Glória; Alves, Lêucio Câmara

doi:10.1007/s00436-016-4950-4

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…However, this present study demonstrates that the FLOTAC technique may be used to diagnose the contamination of lettuces by immature forms of gastrointestinal parasites of animals, and perhaps permit overcoming the limits presented by the classical sedimentation method extensively used. The importance of the FLOTAC technique for the detection of gastrointestinal parasites of animals is indisputable [6,10]; however, the present study opens new possibilities for the use of this technique, beyond its use only in animals.…”

Section: Discussionmentioning

confidence: 91%

An Additional Asset for the FLOTAC Technique: Detection of Gastrointestinal Parasites in Vegetables

Ramos

Giannelli³

et al. 2019

Acta Parasit.

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Introduction Gastrointestinal parasites are considered amongst the most important threats to veterinary and human health worldwide. The transmission of these parasitic diseases usually involves the accidental ingestion of oocysts, cysts, eggs, and larvae, whose detection is generally achieved by classical coproparasitological methods, based on sedimentation and/or flotation techniques. These procedures might be inefficient or display a low sensitivity when performed on food matrices. This study aimed to assess the value of the FLOTAC technique for the detection of different parasitic stages of gastrointestinal parasites of domestic animals in fresh lettuce. Material and methods Samples (n = 100) were purchased from public markets located in the metropolitan region of Recife (Brazil). Results A total of 79% scored positive for eggs and/or cysts of gastrointestinal parasites. Protozoa and nematodes were detected in 32% (32/100) and 64% (64/100) of samples, respectively, with cysts of the genus Entamoeba (32%) and eggs of nematodes of the order Strongylida (30%) being the most frequently diagnosed. Conclusions The findings herein reported demonstrate that the FLOTAC technique can be successfully applied for recovering food-borne parasites of medical and veterinary concern in food matrices.

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Section: Discussionmentioning

confidence: 91%

An Additional Asset for the FLOTAC Technique: Detection of Gastrointestinal Parasites in Vegetables

Ramos

Giannelli³

et al. 2019

Acta Parasit.

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“…They are considered important diagnostic methods for egg detection of gastrointestinal parasites in dogs (Lima et al, 2015;Cringoli et al, 2010), and those techniques were used for detection of C. plica eggs in dogs in Italy (Maurelli et al, 2014). Lima et al, (2016) indicated that FLOTAC could increase the probability of correct diagnosis of urinary capillariosis in dogs. Mini-FLOTAC has shown better results than the classical sedimentation technique and could be used instead of FLOTAC in laboratories that cannot centrifuge urine (Lima et al, 2016).…”

Section: Diagnosismentioning

confidence: 99%

“…Lima et al, (2016) indicated that FLOTAC could increase the probability of correct diagnosis of urinary capillariosis in dogs. Mini-FLOTAC has shown better results than the classical sedimentation technique and could be used instead of FLOTAC in laboratories that cannot centrifuge urine (Lima et al, 2016).…”

Section: Diagnosismentioning

confidence: 99%

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Urinary capillariosis in dogs

Ilić

Rogošić²,

Gajić

et al. 2021

VET GLASNIK

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Background. Urinary capillariosis in dogs is caused by Capillaria plica (syn. Pearsonema plica), a ubiquitous parasitic nematode resembling a string which belongs to the family Capillariidae. It parasitizes the feline, canine and musteline urinary bladder, and has been found in ureters and renal pelvises as well. C. plica has an indirect life cycle, with earthworms (Lumbricina) as intermediate hosts and domestic and wild animals (dog, cat, fox and wolf) as primary hosts. Infection of primary hosts occurs via ingestion of earthworms that contain infective first stadium (L1) larvae. An alternative path of infection for primary hosts is assumed to be ingestion of soil contaminated by infectious larvae derived from decomposed earthworms. Infection is mostly asymptomatic, but the clinical picture presents with pollakiuria, dysuria, haematuria, polydipsia, incontinence and/or fever. Scope and Approach. The aim of this review is to highlight the importance of urinary capillariosis in dogs. Since the health care of wild and domestic carnivores is extremely important, this review provides information about the morphology, biology and epizootiology of the C. plica nematode. Due to the importance of this disease for clinicians and increased disease prevalence during the last decade in many countries, this review presents the latest information on the pathogenesis, clinical signs, diagnosis, treatment and prevention of this infection.

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“…Although the parasite is usually located in one of the kidneys, infection can be bilateral, and worms may also develop to adulthood ectopically in sites such as the abdominal cavity, uterus, ovary, mammary gland, urethra, subcutaneous tissues of the inguinal region, and mesenteric lymph nodes [ 1 , 23 ]. Recent studies have implemented a more sensitive technique for detecting and counting Dioctophyme renale eggs in urine [ 24 ]. Nevertheless, this and other currently used diagnostic methods for dioctophymatosis may fail to detect premature, infertile, single sex, or ectopic infections, and the equipment required makes screening for the infection in rural and economically deprived areas difficult.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale

et al. 2017

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Background: The giant kidney worm, Dioctophyme renale, is a debilitating and potentially lethal parasite that inhabits and destroys, typically host's right kidney, and may also be found in ectopic sites. It is circumglobally distributed, mainly in dogs, and is increasingly regarded as a threat to other domestic animals and humans. There is little information on the parasite's true incidence, or immune responses to it, and none on its biochemistry and molecular biology. Results: We characterised the soluble proteins of body wall, intestine, gonads and pseudocelomic fluid (PCF) of adult parasites. Two proteins, P17 and P44, dominate the PCF of both male and females. P17 is of 16,622 Da by mass spectrometry, and accounts for the intense red colour of the adult parasites. It may function to carry or scavenge oxygen and be related to the 'nemoglobins' found in other nematode clades. P44 is of 44,460 Da and was found to associate with fatty acids by thin layer chromatography. Using environment-sensitive fluorescent lipid probes, P44 proved to be a hydrophobic ligand-binding protein with a binding site that is highly apolar, and competitive displacement experiments showed that P44 binds fatty acids. It may therefore have a role in distributing lipids within the parasites and, if also secreted, might influence local inflammatory and tissue responses. N-terminal and internal peptide amino-acid sequences of P44 indicate a relationship with a cysteine-and histidinerich protein of unknown function from Trichinella spiralis. Conclusions: The dominant proteins of D. renale PCF are, like those of large ascaridids, likely to be involved in lipid and oxygen handling, although there is evidence of strong divergence between the two groups.

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Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs

Cited by 9 publications

References 16 publications

An Additional Asset for the FLOTAC Technique: Detection of Gastrointestinal Parasites in Vegetables

An Additional Asset for the FLOTAC Technique: Detection of Gastrointestinal Parasites in Vegetables

Urinary capillariosis in dogs

Identification and characterization of the major pseudocoelomic proteins of the giant kidney worm, Dioctophyme renale

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