Use of the multi-allelic self-incompatibility gene in apple to assess homozygocity in shoots obtained through haploid induction

Verdoodt, L; Haute, A. Van; Goderis, I.W.M.J.; Witte, K De; Keulemans, Johan; Broothaerts, W.

doi:10.1007/s001220050739

Cited by 72 publications

(57 citation statements)

References 14 publications

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“…Using a S-RNase allele specific polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method developed by us [19,20] and other groups in Belgium [21][22][23][24], China [25], Korea [26][27][28], Israel [29,30], and U.S.A. [31], we have determined the S-RNase genotypes of new apple cultivars and selections produced in Japan to know their cross-compatibility with pollenizers and also to use them in a breeding program. Results and discussion are as follows.…”

Section: Cross-compatibility Among Wild Apples Crab Apples (Pollenizmentioning

confidence: 99%

Apple Pollination Biology for Stable and Novel Fruit Production: Search System for Apple Cultivar Combination Showing Incompatibility, Semicompatibility, and Full-Compatibility Based on theS-RNaseAllele Database

Matsumoto

2014

International Journal of Agronomy

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Breeding and cultivation of new apple cultivars are among the most attractive and important issues for apple researchers. As almost all apple cultivars exhibit gametophytic self-incompatibility (GSI), cross-pollination between genetically different cultivars and species is essential not only for stable fruit production, but also for breeding of new cultivars. For cross-pollination by insect or hand pollination, pollen viability and pistil fertility are key factors, but also the mechanism of GSI has to be taken into account. This paper reviews the germination rate of pollen after storage in different conditions, at different periods of flowering, and in combination with pistil fertility and cross-compatibility among wild-, crab-, and cultivated apples. Furthermore, suitable cultivar combinations for new attractive apple cultivars based on GSI are explored. Especially, details about S-genotypes of apple cultivars, which are present in recent cultivar catalogues, are introduced together with a newly established on-line searchable database of S-genotypes of cultivars, wild apples and crab apples that shows incompatibility, semicompatibility, and full-compatibility.

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Section: Cross-compatibility Among Wild Apples Crab Apples (Pollenizmentioning

confidence: 99%

Apple Pollination Biology for Stable and Novel Fruit Production: Search System for Apple Cultivar Combination Showing Incompatibility, Semicompatibility, and Full-Compatibility Based on theS-RNaseAllele Database

Matsumoto

2014

International Journal of Agronomy

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“…RNase S-alleles from apple cultivars have been previously cloned by several authors Janssens et al, 1995;Sassa et al, 1996;Verdoodt et al, 1998;Matsumoto et al, 1999;Matsumoto and Kitahara, 2000;Nerum et al, 2001;Schneider et al, 2001;Kitahara and Matsumoto, 2002;Broothaerts, 2003). Based on the nucleotide sequences of the S-alleles encoding S-RNases, a PCR on restriction fragment length polymorphism analysis method (PCR-RFLP) for S-allele identification was used (e.g.…”

Section: Self-incompatibility Rnase Allelesmentioning

confidence: 99%

“…We also identified S 6 and S 26 in the 'Maruba Kaido' rootstock and we believe that this is the first time that these alleles have been observed and documented for this variety. S 26 is a rare allele which has been shown to differ from other S-alleles and it has been detected in the crabapple variety 'Baskatong' (M. domestica × niedzwetzkyana), M. floribunda 821 (Verdoodt et al, 1998;Broothaerts, 2003) and in M. × domestica old and M. sylvestris hybrids (Dreesen et al, 2010).…”

Section: Self-incompatibility Rnase Allelesmentioning

confidence: 99%

Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock

Agapito-Tenfen

Dantas

Denardi³

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) is a major insect pest that has significant economic impact on apple growers worldwide. Modern breeding technologies rely on several molecular tools to help breeders select genetic determinants for traits of interest.Consequently, there is a need for specific markers linked to the genes of interest. Apple scions and rootstocks have an additional barrier to the introduction of pest resistance genes due to the presence of self-incompatibility S-RNase alleles. The genetic characterization and early identification of these alleles can amplify the contribution of a breeding program to the selection of resistant genitors that are as compatible as possible. In this study, we identified the Er1 gene involved in the resistance to WAA in Malus prunifolia var. ringo, also known as 'Maruba Kaido' rootstock, and we analyzed the inheritance pattern of the WAA resistance Er1 gene in a segregant population derived from Malus pumila 'M.9' and 'Maruba Kaido' rootstocks. The self-incompatibility of S-RNase alleles S 6 S 26 of 'Maruba Kaido' were also identified along with their inheritance pattern.We also confirmed the identification of the S 1 S 3 alleles in the 'M.9' rootstock. To the best of our knowledge, this is the first study to characterize WAA resistance and RNase S-alleles in 'Maruba Kaido'. Furthermore, we discuss the potential use of the genetic markers for these genes and their potential impact on apple breeding programs.

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“…However, doubled haploid plants were seldom obtained (Germana et al 1994(Germana et al , 2000. In these studies (Germana et al 1994(Germana et al , 2000, the homozygosity of the doubled haploid plants was evaluated by electrophoretic methods, isozyme and DNA analyses, as in the case of other species (Verdoodt et al 1998, Höfer et al 1999, 2002.…”

Section: Introductionmentioning

confidence: 99%

CMA Banding Pattern of Chromosomes Is Useful for the Identification of Chromosome Doubling in Haploid Citrus

Yamamoto

Tominaga

2004

Breed. Sci.

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A variant plant showing long internodes arose from a haploid plant obtained from clementine (Citrus clementina). Flow cytometric analysis and chromosome count revealed that it was a mixoploid with both haploid (2n = x = 9) and diploid (2n = 2x = 18) cells. The average length of the guard cells of the variant was almost the same as that of the haploid plant. This suggested that the epidermis of the variant consisted of haploid cells and that other leaf tissues consisted of diploid cells. Since in citrus the first germ layer forms the epidermis and the second and third layers form all the leaf tissues except for the epidermis, the variant was considered to be a ploidy periclinal chimera. When chromosomes of the young leaves were stained with chromomycin A 3 (CMA), they could be classified into four types based on the number and the position of the CMA-positive bands. The chromosome configuration of the variant differed from that of the original diploid clementine. The number of chromosomes of each type in the diploid cells of the variant was twice the number of those in the haploid plant. These results demonstrated that the diploid cells of the variant were doubled haploid cells, and that CMA analysis of chromosomes is useful for the identification of doubled haploids in citrus.

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Use of the multi-allelic self-incompatibility gene in apple to assess homozygocity in shoots obtained through haploid induction

Cited by 72 publications

References 14 publications

Apple Pollination Biology for Stable and Novel Fruit Production: Search System for Apple Cultivar Combination Showing Incompatibility, Semicompatibility, and Full-Compatibility Based on theS-RNaseAllele Database

Apple Pollination Biology for Stable and Novel Fruit Production: Search System for Apple Cultivar Combination Showing Incompatibility, Semicompatibility, and Full-Compatibility Based on theS-RNaseAllele Database

Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock

CMA Banding Pattern of Chromosomes Is Useful for the Identification of Chromosome Doubling in Haploid Citrus

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