Usefulness of Real-time PCR to Detect<i>Mycobacterium tuberculosis</i>and Nontuberculous Mycobacteria

Yun, Eun Young; Cho, Su Hee; Go, Se Il; Baek, Jong Ha; Kim, You Eun; Eun, Jeong; Lee, Gi Dong; Cho, Yu Ji; Jeong, Yi Yeong; Kim, Ho Cheol; Lee, Jong Deok; Kim, Sun Joo; Hwang, Young Sil

doi:10.4046/trd.2010.69.4.250

Cited by 4 publications

(6 citation statements)

References 19 publications

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“…32,33 The detection limit of acid-fast staining with FFPE specimen is 10 4 bacilli, 8 whereas a positive signal can be detected with only two or more (10 fg) bacilli by the PCR method. 13 Therefore, all acid-fast staining-positive cases are theoretically PCR positive (MTB or NTM PCR). This study concluded that the PCR result has diagnostic significance, particularly for AFB stain-positive specimens.…”

Section: Significance Of the Pcr Results Of Afb Stain-positive Specimensmentioning

confidence: 99%

“…Limited studies have reported on NTM PCR in FFPE samples. 13,20 One showed that 12 cases with positive real-time PCR (six MTB, six NTM) in FFPE specimens revealed a strong correlation with AFB culture (six MTB, five NTM). 6 Another reported that 34% of mycobacterial PCR-positive cases were NTM, but most were not culture-proven cases.…”

Section: Possible Reason For Low Sensitivity Of Real-time Pcr Comparementioning

confidence: 97%

“…13 The Real-Q MTB & NTM Kit (BioSewoom Inc.) was used to perform real-time PCR reactions. The Real-Q MTB & NTM Kit is a ready-to-use kit for the detection of the MTB and mycobacteria in a multiplex real-time PCR assay, based on the TaqMan probe system.…”

Section: Real-time Pcr For Mtb and Ntmmentioning

confidence: 99%

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Clinical Usefulness of PCR for Differential Diagnosis of Tuberculosis and Nontuberculous Mycobacterial Infection in Paraffin-Embedded Lung Tissues

Kim

Choi

et al. 2015

The Journal of Molecular Diagnostics

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The need for isolation of nontuberculous mycobacteria (NTM) from clinical specimens has increased in recent years. Our aim was to determine the clinical usefulness of PCR for differential diagnosis of tuberculosis and nontuberculous mycobacterial infection in lung tissue that show chronic granulomatous inflammation. A total of 199 formalin-fixed, paraffin-embedded specimens, including 137 Mycobacterium tuberculosis (MTB), 17 NTM cases, and 45 other than mycobacterial cases were collected. We performed acid-fast staining, MTB and NTM nested PCRs, and MTB and NTM real-time PCRs. No histologic difference between MTB and NTM infections was observed. Sensitivity and specificity for detecting MTB were 70.1% and 95.1% by nested PCR, respectively, and 70.8% and 100.0% by real-time PCR, respectively. Sensitivity and specificity for detecting NTM were 52.9% and 96.15% by nested PCR, respectively, and 35.3% and 100.0% by real-time PCR, respectively. Mycobacteria were identified by acid-fast staining in 50 of 154 cases (32.5%). All 50 acid-fast staining-positive cases showed positive nested and real-time PCR results (n = 47 MTB PCR positive; n = 3 NTM PCR positive), and results agreed with final diagnosis. PCR will be useful for the rapid diagnosis of mycobacterial infection and differentiation of MTB from NTM in formalin-fixed, paraffin-embedded specimens, especially in acid-fast staining-positive specimens.

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Section: Significance Of the Pcr Results Of Afb Stain-positive Specimensmentioning

confidence: 99%

Section: Possible Reason For Low Sensitivity Of Real-time Pcr Comparementioning

confidence: 97%

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Clinical Usefulness of PCR for Differential Diagnosis of Tuberculosis and Nontuberculous Mycobacterial Infection in Paraffin-Embedded Lung Tissues

Kim

Choi

et al. 2015

The Journal of Molecular Diagnostics

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“…Therefore, the combined use of liquid and solid media is recommended for quick and accurate verification 2,4 . TB-specific polymerase chain reaction (PCR) has been widely used to overcome Original Article the flaws of stains and culture tests but it is unable to detect NTM 5 . Recently, TB/NTM real-time PCR which can detect both TB and NTM was introduced and has been increasingly used 5,6 .…”

Section: Introductionmentioning

confidence: 99%

“…TB-specific polymerase chain reaction (PCR) has been widely used to overcome Original Article the flaws of stains and culture tests but it is unable to detect NTM 5 . Recently, TB/NTM real-time PCR which can detect both TB and NTM was introduced and has been increasingly used 5,6 . Furthermore, the identification of NTM species using multiplex PCR or DNA chip became possible.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Ogawa Media, BACTEC MGIT 960 System and TB/NTM Real-Time PCR for DetectingMycobacteriumSpecies

2011

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Background: Mycobacterial infection is a problem throughout the world along with the increase of immunocompromised patients. For this reason, there have been many methods for faster and more accurate diagnosis. In this study, we evaluated several laboratory methods for mycobacterial infection. In the Mycobacteria genotyping chip analysis, the most frequently identified NTM species in descending order were M. avium, M. intracellulare, M. chelonae and M. abscessus. Conclusion: Culturing with a combination of MGIT and Ogawa is recommended to increase the recovery rate of mycobacteria. Although PCR missed a reasonable number of NTM, it is faster and usually gives results that concur with those from the culture. The appropriate combination of diagnostic methods with clinical correlation are necessary.

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Nontuberculous mycobacterial pulmonary disease mimicking lung cancer

et al. 2016

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To describe the features and clinical implications of computed tomography (CT), positron emission tomography (PET), and percutaneous needle aspiration biopsy (PCNB) in pulmonary nontuberculous mycobacterial (NTM) disease manifesting as a solitary nodule, mass, or mass-like consolidation mimicking malignancy.Among a cohort of 388 patients with NTM pulmonary disease, 14 patients with clinically and radiologically suspected lung cancer were included in our study. Two chest radiologists evaluated CT features, including lesion type (nodule, mass, or mass-like consolidation), morphologic features (margin, degree of enhancement, calcification), and presence of accompanying findings suggestive of NTM pulmonary disease (bronchiectasis with clustered centrilobular nodules or upper-lobe cavitary lesions) by consensus. Diagnostic procedures for microbiologic diagnosis of NTM disease and clinical outcome were reviewed.Incidence of NTM pulmonary disease presenting as solitary nodule/mass (n = 8) or mass-like consolidation (n = 6) was 3.6% (14 of 388). Most lesions were detected incidentally during routine health check-up or evaluation of other disease (11 of 14, 79%). Lesions typically showed poor contrast-enhancement (9 of 12) and internal calcification (6 of 14). No lesions had CT features suggestive of NTM pulmonary disease. All 4 lesions for which PET/CT imaging was performed showed strong fluorodeoxyglucose uptake simulating malignant lesions (mean, 4.9; range, 3.6–7.8). PCNB revealed mycobacterial histology in 6 of 11 specimens and positive culture results were obtained for 7 of 7 specimens.NTM pulmonary disease may present as a solitary nodule, mass, or mass-like consolidation mimicking malignancy. CT features and PCNB are important to diagnose NTM disease mimicking lung cancer to avoid unnecessary surgery.

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Usefulness of Real-time PCR to DetectMycobacterium tuberculosisand Nontuberculous Mycobacteria

Cited by 4 publications

References 19 publications

Clinical Usefulness of PCR for Differential Diagnosis of Tuberculosis and Nontuberculous Mycobacterial Infection in Paraffin-Embedded Lung Tissues

Clinical Usefulness of PCR for Differential Diagnosis of Tuberculosis and Nontuberculous Mycobacterial Infection in Paraffin-Embedded Lung Tissues

Comparison of Ogawa Media, BACTEC MGIT 960 System and TB/NTM Real-Time PCR for DetectingMycobacteriumSpecies

Nontuberculous mycobacterial pulmonary disease mimicking lung cancer

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