Using 1-propanol to significantly enhance the production of valuable odd-chain fatty acids by Rhodococcus opacus PD630

Zhang, Lin-Shang; Xu, Pei; Chu, Mei-Yun; Zong, Min‐Hua; Yang, Juan; Lou, Wen‐Yong

doi:10.1007/s11274-019-2748-0

Cited by 24 publications

(18 citation statements)

References 24 publications

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“…The respective C17:1 content in lipids was 31.62% ± 1.15 and 31.62% ± 0.94, which was non-significantly different (p < 0.05) from the predicted value found using NEM-RODW software (34.7% ± 2.31). The C17:1 obtained was higher than that reported by Zhang et al (the highest value reported was 26.42% ± 0.03) [44] and lower than that reported by Kolouchová et al (37% but low lipid content of 0.23 g/L) [45]. OCFA content in both cultures represents ≈ 60% of the total fatty acids in lipids, which corresponds to ≈ 1.…”

Section: Fermentation Under the Optimal Culture Conditionscontrasting

confidence: 60%

Optimization of cis-9-Heptadecenoic Acid Production from the Oleaginous Yeast Yarrowia lipolytica

et al. 2022

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Odd-chain fatty acids (OCFA) have been studied for their therapeutic and nutritional properties, as well as for their potential use in the chemical industry for the production of biofuel. Genetic modification strategies have demonstrated an improved production of OCFA by oleaginous microorganisms. In this study, the production of OCFA-enriched lipids by fermentation using a genetically engineered Yarrowia lipolytica strain was investigated. The major fatty acid produced by this strain was the cis-9-heptadecenoic acid (C17:1). Its biosynthesis was optimized using a design of experiment strategy involving a central composite design. The optimal responses maximizing the cell density (optical density at 600 nm) and the C17:1 content (%) in lipids were found using 52.4 g/L sucrose, 26.9 g/L glycerol, 10.4 g/L sodium acetate, 5 g/L sodium propionate, and 4 g/L yeast extract. Under these conditions, in a 5 L scale bioreactor, the respective contents of lipids and C17:1 in culture medium were 2.52 ± 0.05 and 0.82 ± 0.01 g/L after 96 h fermentation. The results obtained in this work pave the way toward the process upscale of C17:1 and encourage its industrial production.

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Section: Fermentation Under the Optimal Culture Conditionscontrasting

confidence: 60%

Optimization of cis-9-Heptadecenoic Acid Production from the Oleaginous Yeast Yarrowia lipolytica

et al. 2022

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“…Thus, SCO from oleaginous rhodococci can be considered promising feedstock for biodiesel production because of their similar fatty acid composition to vegetable oils [ 61 ]. Curiously, an unusually high fraction of fatty acids with an odd number of carbon atoms occurs in the accumulated TAG during the cultivation of cells on sugars or organic acid as sole carbon sources [ 62 , 63 ]. The amounts of odd-numbered fatty acids depend on the substrates used as carbon sources.…”

Section: Catabolism Of Different Carbon Sources and Lipogenesis Pathways Are Coupled In Oleaginous Rhodococcimentioning

confidence: 99%

“…For this reason, the ability of the oleaginous rhodococcal strains to synthesize and incorporate this type of fatty acid into TAG is an important differential property in relation to other SCO-producing microorganisms, which naturally produce negligible amounts of odd chain fatty acids, such as Yarrowia lipolytica , among others [ 65 ]. Odd chain-length fatty acids are considered valuable products for their applications in the medical, therapeutic, and nutritional industries, as well as in chemical industries as precursors for manufacturing agricultural chemicals such as biocides, flavor and fragrance intermediates, hydraulic fluids, plasticizers, and coatings, among other applications [ 63 , 65 , 66 ]. Thus, oleaginous rhodococci can be considered a biological source of valuable oils, which are not readily obtainable via traditional petrochemical processes, with wide industrial applications.…”

Section: Catabolism Of Different Carbon Sources and Lipogenesis Pathways Are Coupled In Oleaginous Rhodococcimentioning

confidence: 99%

“…Chu et al have recently found that R. opacus PD630 is able to produce up to 48% of CDW of oil enriched in odd-numbered fatty acids from 1-propanol and glucose as carbon and energy sources [ 68 ]. In addition, the amount of accumulated TAG reached up to 55% of CDW after increasing 1-propanol concentration in the cell cultures [ 63 ]. Transcriptomic analyses revealed the redirecting of carbon flow from the methylmalonyl-CoA pathway toward the generation of propionyl-CoA, which is used for de novo odd-numbered fatty acids biosynthesis [ 63 ].…”

Section: Catabolism Of Different Carbon Sources and Lipogenesis Pathways Are Coupled In Oleaginous Rhodococcimentioning

confidence: 99%

“…In addition, the amount of accumulated TAG reached up to 55% of CDW after increasing 1-propanol concentration in the cell cultures [ 63 ]. Transcriptomic analyses revealed the redirecting of carbon flow from the methylmalonyl-CoA pathway toward the generation of propionyl-CoA, which is used for de novo odd-numbered fatty acids biosynthesis [ 63 ]. Kosa and Ragauskas reported that R. opacus DSM1069 is able to use coniferyl alcohol as a carbon source and synthesize TAG from it [ 69 ].…”

Section: Catabolism Of Different Carbon Sources and Lipogenesis Pathways Are Coupled In Oleaginous Rhodococcimentioning

confidence: 99%

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Rhodococcus as Biofactories for Microbial Oil Production

et al. 2021

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Bacteria belonging to the Rhodococcus genus are frequent components of microbial communities in diverse natural environments. Some rhodococcal species exhibit the outstanding ability to produce significant amounts of triacylglycerols (TAG) (>20% of cellular dry weight) in the presence of an excess of the carbon source and limitation of the nitrogen source. For this reason, they can be considered as oleaginous microorganisms. As occurs as well in eukaryotic single-cell oil (SCO) producers, these bacteria possess specific physiological properties and molecular mechanisms that differentiate them from other microorganisms unable to synthesize TAG. In this review, we summarized several of the well-characterized molecular mechanisms that enable oleaginous rhodococci to produce significant amounts of SCO. Furthermore, we highlighted the ability of these microorganisms to degrade a wide range of carbon sources coupled to lipogenesis. The qualitative and quantitative oil production by rhodococci from diverse industrial wastes has also been included. Finally, we summarized the genetic and metabolic approaches applied to oleaginous rhodococci to improve SCO production. This review provides a comprehensive and integrating vision on the potential of oleaginous rhodococci to be considered as microbial biofactories for microbial oil production.

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Efficient Production of 1,3‐Dioleoyl‐2‐Palmitoylglycerol through Rhodococcus opacus Fermentation

Zhang

Chu

Zong

et al. 2020

J Americ Oil Chem Soc

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Abstract1,3‐Dioleoyl‐2‐palmitoylglycerol (OPO) is the main triacylglycerol species in human milk‐fat substitute. The production of OPO is of considerable research interest. In this study, a new strategy for producing OPO by fermentation with R. opacus is proposed. Chemically Interesterified fat (high oleic acid sunflower oil/hydrogenated palm oil 1.73:1 w/w), or a mixture of ethyl oleate/ethyl palmitate 2:1 (w/w) as a starting material. The highest biomass and oil content obtained were 3.3 g L−1 and 40.2% (dry cell weight), respectively. The yield of OPO was 0.62 g L−1. The fatty acid composition of produced OPO was 55.7–59.7% 18:1 and 28.3–29.8% 16:0, and the sn‐2 position was predominantly 16:0 (64.7–74.5%). 13C‐nuclear magnetic resonance analysis showed that the sn‐1,3 and sn‐2 positions were predominately esterified by 18:1 and 16:0, respectively. OPO (47.1%), OPL (13.9%), PPO (13.1%), and PPoO (16:0–16:1–18:1) (10.3%) were the most abundant triacylglycerol species.

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Using 1-propanol to significantly enhance the production of valuable odd-chain fatty acids by Rhodococcus opacus PD630

Cited by 24 publications

References 24 publications

Optimization of cis-9-Heptadecenoic Acid Production from the Oleaginous Yeast Yarrowia lipolytica

Optimization of cis-9-Heptadecenoic Acid Production from the Oleaginous Yeast Yarrowia lipolytica

Rhodococcus as Biofactories for Microbial Oil Production

Efficient Production of 1,3‐Dioleoyl‐2‐Palmitoylglycerol through Rhodococcus opacus Fermentation

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