Using electrophoretic deposition to identify protein charge in biological medium

Abstract

Protein adsorption is the first step involved in establishing a suitable integration between a biomaterial and host tissue. It is therefore of highest interest to know the electric charge of proteins present in the relevant medium to be able to predict the behaviour of cells on given surfaces. In this study, electrophoretic deposition (EPD) was used as a simple method to identify the charge of proteins present in biological medium. In the model experiment carried out here, EPD was conducted using a biological medium containing 10% fetal calf serum (FCS) and the charge of the protein was determined by examining the migration of the protein in the EPD cell under a certain applied voltage. In addition, the suitability of EPD of proteins to deliver tailored surfaces for enhanced bioactivity or for controlled deposition of protein films on metallic surfaces was explored.

Electrophoretic Deposition of Biological Macromolecules, Drugs, And Cells

“…As protein adsorption is the first step when a biomaterial surface gets in contact with the host tissue, Meng et al 89 used EPD of biological medium containing 10% fetal calf serum (FCS) to investigate the electric charge of proteins in medium. This knowledge is important to understand the behavior of cells on biomaterials and to explore protein deposition on surfaces for functionalization.…”

Electrophoretic deposition of cobalt oxide nanoparticles on aluminium substrate

“…EPD is a technique where charged, colloidal particles or large molecules suspended in a liquid medium migrate under the influence of an electric field by the application of a DC voltage onto an oppositely charged electrode depositing on the substrate. Particles must be electrically charged to permit film formation by EPD and also for the stabilization of the suspension [10][11][12].…”

Electrophoretic Coating of Octahedral Molybdenum Metal Clusters for UV/NIR Light Screening

“…The effective application of the EPD process has been extensively studied for the fabrication of traditional ceramic materials for a long time [22,23]. In recent studies, the EPD process has been extended to graphene-related materials [24], nanoscale TiO 2 [25], or medical materials, i.e., antimicrobial applications [26], bond tissue engineering [27], protein identification [28], and bio-implants [29], based on the possible control of the thickness on a nanometer scale.…”