Using Scrapings from Formalin-Fixed Tissues to Diagnose Leptospirosis by Fluorescent-Antibody Techniques

Cook, James E.; Coles, Embert H.; Garner, F. M.; Luna, Lee G.

doi:10.3109/10520297109067874

Cited by 3 publications

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“…l Tissues of various domestic and wild animals have been reported to be FAT-positive when tested with canicola, icterohaemorrhagiae, and pomona conjugates. 9,15 It has been proposed that autolyzed, dehydrated, or mummified fetuses 18 and formalin-fixed tissues 3 can be satisfactory specimens for the FAT. The FAT has been investigated as a serotyping method that would eliminate the time required for growth of isolates and absorption tests.…”

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confidence: 99%

Evaluation of Multivalent Leptospira Fluorescent Antibody Conjugates for General Diagnostic Use

1989

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Abstract. Four lots of conjugate were evaluated for optimal dilution and degree of fluorescence produced with reference cultures and bovine and porcine leptospira isolates. One lot that uniformly produced better fluorescence was evaluated for sensitivity and specificity with reference cultures, isolates, culture-positive tissues, and 13 other bacterial species. Further evaluation of the conjugates was done with bovine, porcine, and ovine specimens submitted to a diagnostic laboratory. Leptospires were detected with the fluorescent antibody test (FAT) in 9 of 21 culture-positive bovine kidneys and were detected in diluted cultures when present at concentrations of 10 2 -1O 3 organisms/ml. With the exception of Treponema hyodysenteriae, FAT's of other bacterial cultures produced minimal fluorescence or were negative. Positives were characterized by moderate to brilliant fluorescence of typical cell forms, and most nonspecific fluorescence was eliminated with a flazo-orange counterstain. The results indicated that the FAT utilizing multivalent conjugates could be used successfully as an additional method for diagnosis of leptospira infections.

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mentioning

confidence: 99%

Evaluation of Multivalent Leptospira Fluorescent Antibody Conjugates for General Diagnostic Use

1989

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Laboratory Diagnosis of Leptospirosis

Shotts

1976

Biology of Parasitic Spirochaetes

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Immunofluorescent Staining of Leptospires in Pepsin Treated Histologic Sections

Skilbeck

1986

Stain Technology

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A standard immunofluorescent method was modified for the staining of leptospires in formalin fixed, paraffin embedded tissues. Routine histologic sections were deparaffinized and treated with pepsin prior to staining. Pepsin treatment greatly enhanced subsequent staining of leptospires in naturally infected bovine and porcine tissues as well as in artificially infected tissues. Leptospires in naturally infected bovine tissues were usually undetectable in untreated sections but clearly visible in stained pepsin-treated sections. Naturally infected porcine kidney usually contained high levels of leptospiral antigen which could be stained without prior pepsin treatment. However, pepsin treatment of porcine tissues greatly increased the amount of leptospiral antigen detectable and made individual leptospires more conspicuous. The staining method could employ a single antiserum for the staining of leptospires from 13 serogroups. Also, leptospires could be stained in tissues stored in formalin for more than 14 months and in 26-year-old paraffin embedded tissues.

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Using Scrapings from Formalin-Fixed Tissues to Diagnose Leptospirosis by Fluorescent-Antibody Techniques

Cited by 3 publications

References 1 publication

Evaluation of Multivalent Leptospira Fluorescent Antibody Conjugates for General Diagnostic Use

Evaluation of Multivalent Leptospira Fluorescent Antibody Conjugates for General Diagnostic Use

Laboratory Diagnosis of Leptospirosis

Immunofluorescent Staining of Leptospires in Pepsin Treated Histologic Sections

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