Deoxyribonucleic acids (DNAs) from an unnamed species of microorganism (bovine encephalitis isolate) previously placed in the family Brucellaceae and from representative species of this family were thermally denatured t o determine mole percentages of guanine plus cytosine (mol %G+C). The results established that DNA from the bovine encephalitis isolate, had a mol %G+C of 37.3 * 0.2, which was between values for Haemophilus influenzae and Francisella tularensis. Values for mol %G+C of the other microorganisms in this family agreed with previously reported data. A heterogenous group of microorganisms composed of six different overlapping genera and the BE1 were in the range of 3 3 t o 46 mol %G+C. Because the mol %G+C of the genera in this group did not differ statistically, the proper taxonomic position of recognized members of the family Brucellaceae was questioned.

The use of Pagano Levin medium for primary isolation of Candida species from highly contaminated specimens proved unsatisfactory owing to overgrowth by bacteria and fungi. Addition of cycloheximide and chloramphenicol to the medium greatly improved results by inhibiting overgrowth. The additional inhibitors also altered rate of growth and degree of pigmentation reported for some of the Candida species. Complete Pagano Levin medium contains Pagano Levin base (Difco) plus 2,3, 5-triphenyltetrazolium chloride (TTC; Difco), 0.1 mg/ml, and neomycin (The Upjohn Co., Kalamazoo, Mich.), 0.5 mg/ml. It has been recommended as

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