Using the enzymatic and non-enzymatic antioxidant defense system of the land snail Eobania vermiculata as biomarkers of terrestrial heavy metal pollution

El-Shenawy, Nahla S.; Mohammadden, Amaal; AL-Fahmie, Z. H. H.

doi:10.1016/j.ecoenv.2012.08.014

Cited by 50 publications

(13 citation statements)

References 34 publications

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“…Induction of CAT and/or GPx is a protective response by the snail's system to combat the oxidative stress effects of hydrogen peroxide a product of the dismutation of superoxide anion radical produced during normal metabolism of pesticides [22]. Our results are consistent with the findings of [23] who also observed elevated CAT and GPX activities in snails exposed to metals such as lead, cadmium, iron and copper. Radwan et al [18] also reported activation of CAT, GPX and GST in Theba pisana snails exposed to the heavy metals; lead, zinc, copper and cadmium.…”

Section: Discussionsupporting

confidence: 82%

Pollutants, Snails, Oxidative-Stress, Organophosphates, Metals

Basopo¹,

Ngabaza²

2015

ABC

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Aquatic reservoirs remain the ultimate sink of chemical pollutants emanating from anthropogenic activities such as agriculture, mining and industry. Freshwater biota undoubtedly is at risk from the adverse effects of these water pollutants and there is therefore, a need to monitor effects of these chemical pollutants in order to safeguard the health of aquatic biota. We investigated the oxidative stress effects of chlorpyrifos and lead on the freshwater snail Helisoma duryi to assess the potential of using this enzyme system as a biondicator of exposure to environmental pollutants. Groups of snails were exposed to 5 ppb lead acetate and 25 ppb chlorpyrifos for 7 days after which half of the snails were sacrificed and the other half were allowed to recover in clean water and sacrificed after another 7 days. Post mitochondrial fractions were used to measure the activities of the following antioxidant enzymes: superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and diphosphotriphosphodiaphorase. Both pollutants enhanced the activities of all the antioxidant enzymes suggesting a defensive mechanism by the snail to combat the oxidative stress due to the organophosphate chlopryrifos and metal pollutant lead. There was a significant recovery of the antioxidant defense system of the snails allowed to recover in clean water shown by the reduced alteration of the antioxidant enzyme activities of the snails allowed to recover for 7 days. This suggests the need to minimize exposure of aquatic biota to chemical pollutants and remediate the polluted water reservoirs in order to safeguard the health of aquatic life.

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Section: Discussionsupporting

confidence: 82%

Pollutants, Snails, Oxidative-Stress, Organophosphates, Metals

Basopo¹,

Ngabaza²

2015

ABC

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“…These findings were consistent with the current evidence, which showed that elevated Pb levels increased LPO levels in the digestive gland of polluted-site samples. Increasing LPO levels during exposure to metals have been recorded in several organisms (Radwan et al, 2010; El-Shenawy et al, 2012). Several studies have looked into the toxic effects of Pb on membrane components and have discovered a connection between these effects and oxidative damage caused by Pb.…”

Section: Discussionmentioning

confidence: 99%

“…Land snails are dispersed in Egypt in different governorates, which include Alexandria, Kafr El-Sheik, Behera, Damietta, Ismailia, Sharkia, Monofia, Gharbia, Minia, and Assiut (Shoieb, 2008; Ramzy, 2009; Eshra, 2013; Abdelgalil et al, 2018; Desoky, 2018). Many scholars have looked at ecological studies such as surveys, population dynamics and migration, daily life, and ground snail dispersal (Ramzy, 2009; El-Shenawy et al, 2012; Desoky et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Due to their capacity to absorb trace metals in their tissues, land snails such as H. aspersa and Arianta arbustorum have historically been used to estimate urban or industrial waste (Regoli et al, 2006; Itziou and Dimitrids, 2009). E. vermiculata was found to be an appropriate sentinel organism, and the biomarkers chosen are effective for terrestrial heavy metal biomonitoring in Taif (El-Shenawy et al, 2012) as well as Ismailia (Ghobashy et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Impact of contamination on biology, biochemical, and histology ofEobania vermiculataandMonacha obstructaduring different seasons in Ismailia, Egypt

Ghobashy

Al-Thomali

Soliman

et al. 2021

Preprint

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Land snails are found to be an appropriate sentinel organism, and the biomarkers chosen are effective for terrestrial heavy metal biomonitoring. The study aimed to compare the biological, biochemistry, and histology of two land snails in the Ismailia governorate, Egypt during different seasons. Random snails were collected from January 2015 to December 2015 from two sites in the Ismailia Governorate, on wet days during each season's middle month. Soft tissues were taken from the dissected snails. It was noticed that most of the morphometric parameters measured shell height (ShH), last whorl width (LWW), maximum diameter (MaxD), aperture height (AH), and aperture width (AW) were higher in Eobania vermiculata (Sp. 1) than those measured in Monacha obstructa (Sp. 2), except for shell height measurement, which was the same in both species. The growth rate of Sp. 1 and Sp. 2 changed seasonally. In the more polluted areas with heavy metals, lipid peroxidation (LPO) was higher in snails and total protein content than in the snails collected from the less polluted areas for all seasons. However, the snails displayed lower levels of glutathione (GSH) as compared to snails at a less polluted site. GSH and LPO levels, on the other hand, have a negative relationship between them. Histopathological alterations in the digestive gland were more obvious in the general architecture of the digestive gland that had lost its tubular appearance. The excretory cells showed an increase in their excretory granules' number and size while calcium cells decreased. Also, gonad follicles have lost their normal architecture with the degeneration of some stages of spermatogenesis and oogenesis. In conclusion, There was a strong correlation between GSH levels and total protein content in the same soft tissues. GSH and LPO levels, on the other hand, have a negative relationship. The overall results display the usefulness of E. vermiculata and M. obstructa land snails as bioindicator organisms and support the application of this ecotoxicological approach for evaluating the biologic impact of toxins. E. vermiculata is more abundant than M. obstructa . The density, morphometric, biochemical, and histology of E. vermiculata and M. obstructa were different at different seasons.

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“…Among antioxidant parameters e.g. SOD, CAT, GST, GPx and LPO are extensively studied [2,[13][14]. Recently, antioxidant enzymes and the contents of malondialdehyde (MDA) have been used as biomarkers of pollutants that generate oxidative stress in aquatic animals [15][16].…”

Section: Introductionmentioning

confidence: 99%

Comparative toxicity of abamectin and nano-derived form on land snail, Helix aspersa in attributing to cytotoxicity and biochemical alterations

Abdel-Halim¹,

Osman²,

El-Danasoury³

et al. 2021

World J. Adv. Res. Rev.

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The comparative toxic effect of Vertimec® 1.8% EC, Fast Max Super® 8.4% SC and nano-derived form of abamectin (ABM) (1% nano-emulsion) as a dermal contact for 48 h against land snail, Helix aspersa was evaluated at laboratorial trail. Acute toxicity values (LD50) were 6.45, 11.97, and 45.95 µg snail-1 for nano-derived form of ABM, Fast Max Super® and Vertimec®, respectively. Nano-derived form exhibited the highest toxic effects (1.86 and 7.12-folds), respect to Fast Max Super® and Vertimec®. Sublethal doses: 1/10 and 1/100 LD50s of the examined compounds were applied to evaluate some biochemical alterations e.g. acetylcholinesterase (AChE), malondialdhyde (MDA), lactate dehydrogenase (LDH), glutathione-S-transferase (GST), acid phosphatase (ACP), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), respectively, in haemolymph and digestive glands homogenates. The all treatments significantly decreased AChE activity in ganglia homogenate, respect to control group (untreated). All treatments exhibited MDA level and LDH activity greater than the control in both haemolymph and digestive gland. This concept recognizes the cytotoxic effect of ABM on gastropods. Significant declines in GST, ACP, and ALP activities were exhibited in homogenate of digestive gland for the all treatments. However, AST/ALT activities exhibited increase greater than untreated group. These findings may explain the role of these doses of ABM for dysfunction in organs of H. aspersa. Thus, prepared nano-emulsion was more potent toxic on land snails. However, H. aspersa is considered a useful tool to assess ecotoxicological impact of pesticides.

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Using the enzymatic and non-enzymatic antioxidant defense system of the land snail Eobania vermiculata as biomarkers of terrestrial heavy metal pollution

Cited by 50 publications

References 34 publications

Pollutants, Snails, Oxidative-Stress, Organophosphates, Metals

Pollutants, Snails, Oxidative-Stress, Organophosphates, Metals

Impact of contamination on biology, biochemical, and histology ofEobania vermiculataandMonacha obstructaduring different seasons in Ismailia, Egypt

Comparative toxicity of abamectin and nano-derived form on land snail, Helix aspersa in attributing to cytotoxicity and biochemical alterations

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