Using the pER8:GUS Reporter System to Screen for Phytoestrogens from <i>Caesalpinia sappan</i>

Lai, Wan Chun; Wang, Hui‐Chun; Chen, Guan Yu; Yang, Juan; Korinek, Michal; Hsieh, Chia‐Jung; Nozaki, Hirōshi; Hayashi, Ken‐ichiro; Wu, Chih Chung; Wu, Yang Chang; Chang, Fang Rong

doi:10.1021/np100920q

Cited by 30 publications

(25 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T47D cells have a mutation of p53 and express estrogen receptor (ER) (Schafer, et al, 2000). Compounds isolated from methanolic extract of C. sappan exhibited partial antiestrogenic activity on MCF-7 cells (Lai, et al, 2011). Upregulation of p21 might occurred through p53 independent pathway leading to inhibition of cell cycle .…”

Section: Discussionmentioning

confidence: 96%

“…Methanolic extract of C. sappan exhibited cytotoxicity (IC 50 <20 μg/mL) against HepG2 liver, MCF-7 and MDA-MB-231 breast, and A549 lung cancer cell lines (Lai, et al, 2011). Yen, et al (2010 reported that brazilein isolated from secang showed potent cytotoxic effects on Hep G2 and Hep 3b liver cancer cells, MDA-MB-231 and MCF-7 breast cancer cells, and A549 lung cancer cells.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

Rivanti

Shabrina

Nurzijah

et al. 2017

IJCC

View full text Add to dashboard Cite

The present study investigate the selectivity of heartwood of secang ethanolic extract (SEE) on T47D breast cancer cells, WiDr colon cancer cells, and HeLa cervical cancer cells, compared to Vero normal epithelial cells. The cytotoxic effect was evaluated by using MTT assay with 24-hour treatment to get IC50 values. Selectivity was evaluated by using selectivity index (SI). SEE had a potent cytotoxic activity on T47D and WiDr cancer cells (IC50<100 µg/mL). IC50 value of HeLa cancer cells was observed on moderate cytotoxic (1003), while in HeLa cells is not selective (SI<3). This result indicating its potential of Caesalpinia sappan as a chemopreventive agent in cancer therapy.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

Rivanti

Shabrina

Nurzijah

et al. 2017

IJCC

View full text Add to dashboard Cite

show abstract

“…Shengqian (2011) reported that EES significantly suppressed IL1β-mediated upregulation of MMP-13 mRNA and protein levels via abrogation of the NF-κB (p65/p50)-driven MMP-13 promoter activation. Extract of Caesalpinia sappan L. shows significant estrogenic effect through increasing SEAP (Secreted Alkaline Phosphatase) activity in MCF-7 cells (Lai, et al, 2011). Thus, EES mechanism inhibiting osteoclast formation can be caused of NF-κB pathway alteration.…”

Section: Effects Of Ees On Osteoclast Formationmentioning

confidence: 99%

“…Beside brazilin and brazilein, compounds in EES perform as phytoestrogen, such as deoxysappanone, deoxysappanchalcone, and protosappanin (Lai, et al, 2011). Therefore, those compounds might perform as exogenous estrogen in postmenopausal women.…”

Section: Effects Of Ees On Osteoclast Formationmentioning

confidence: 99%

Ethanolic Extract of Secang Heartwood (Caesalpinia sappan L.) Increases Bone Density in Ovariectomized Rats

Shabrina

Kristiani

Irnanda

et al. 2013

Indones J Cancer Chemoprevent

View full text Add to dashboard Cite

Osteoporosis has become a major public health concern, due to its rising incidence every year. Osteoporosis mostly occurs in women at menopause, especially in Asia. Secang (Caesalpinia sappan L.) heartwood contains brazilin and brazilein which are known to suppress expression of NF-ĸB, an osteoclastogenesis protein playing an important role in osteoporosis. Thus, the aim of this research is to investigate the inhibition of osteoporosis by ethanolic extract of secang (Caesalpinia sappan L.) (EES) in ovariectomized (OVX) rats. Thirty female Sprague Dawley rats were divided into five groups treated for 20 days: group I were OVX and treated with 1000 mg/kgBW EES; group II were OVX and treated with 500 mg/kgBW EES; group III were OVX and treated with CMC-Na; group IV were shame OVX baseline; and group V were non-OVX-baseline. Bone density was examined by using X-Ray rontgen and was strengthen by qualitative data of Giemsa staining. Moreover, total number of osteoclasts in bone slide was observed by TRAP expression using double staining immunohistochemistry with hematoxylin eosin (HE)-TRAP. Treatment of 500 mg/kgBW EES in ovariectomized rats showed the highest bone density of 0.60 gr/cm 3 and the lowest number of osteoclasts was 4.66 osteoclasts per field of view. These results showed that EES is potent to be developed as an antiosteoporosis agent. Further study on its dose adjustment and molecular mechanism needs to be conducted.

show abstract

“…The transgenic plant pER8:GUS, with the GUS gene as a gene fusion marker for the analysis of gene expression, expresses high estrogenic sensitivity and can be used to quantify the bioactivity of phytoestrogens (Lai et al, 2011). Moreover, it is a visible system, and primary results can be readily observed visually, without the need of special instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity guided isolation of phytoestrogenic compounds from Cyclopia genistoides by the pER8:GUS reporter system

Roza

Lai

Zupkó

et al. 2017

South African Journal of Botany

View full text Add to dashboard Cite

The popular South African herbal tea, honeybush, is made from several Cyclopia species (family: Fabaceae), amongst them Cyclopia genistoides. Phytoestrogenic potential of C. genistoides. has been recently reported, however bioactivity-guided isolation of compounds with estrogenic activity has not yet been performed. A transgenic plant system, Arabidopsis thaliana pER8:GUS, was used to assay the estrogen-like activity of C. genistoides. The quantitative determination of the active compounds in the fermented and non-fermented plant material was performed by HPLC. Subsequent bioactivity-guided fractionation led to the isolation of genistein, naringenin, isoliquiritigenin, luteolin, helichrysin B and 5,7,3′,5′-tetrahydroxyflavanone, four of them first reported in the genus. Helichrysin B, naringenin and 5,7,3′,5′-tetrahydroxyflavanone differed in quantity in the fermented and unfermented herbs, the fermented plant material contained two compounds with substantial estrogenic-like activity in higher concentration (naringenin and 5,7,3′,5′-tetrahydroxyflavanone), whereas the less active helichrysin B was more abundant in the unfermented herb. The fractions as well as compounds inhibited the growth of human cancer cell lines A2780 and T47D. These results underline the phytoestrogenic activity of C. genistoides and support the rationale to the fermentation process.

show abstract

Using the pER8:GUS Reporter System to Screen for Phytoestrogens from Caesalpinia sappan

Cited by 30 publications

References 32 publications

Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

Ethanolic Extract of Secang Heartwood (Caesalpinia sappan L.) Increases Bone Density in Ovariectomized Rats

Bioactivity guided isolation of phytoestrogenic compounds from Cyclopia genistoides by the pER8:GUS reporter system

Contact Info

Product

Resources

About