Utility of cell block in the cytological preoperative diagnosis of keratocystic odontogenic tumor

Rivero, Elena Riet Correa; Grando, Liliane Janete; Ramos, Grasieli de Oliveira; Belatto, Maria Fernanda da Silva; Daniel, Filipe Ivan

doi:10.1016/j.prp.2013.12.006

Cited by 10 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of acellular amorphous eosinophilic materials, detected in 55% of our samples, had not been observed in our previous studies with KCOT and cystic lesions, 5,6,7 what makes us to conjecture about an epithelial origin. However, to completely understand the nature and origin of this material it would be needed a detailed IHC or electron microscopy study.…”

Section: 67contrasting

confidence: 58%

“…5,6,7 The cytological analysis performed using materials prepared by the cell block technique provides an easy recognition of cells because the cytomorphological features remain well preserved after processing. 8 An additional advantage of this tech- Ameloblastoma Unicystic Ameloblastoma nique is the possibility of executing special stains, such as IHC, to identify structures.…”

Section: Discussionmentioning

confidence: 99%

“…5,6,7 According to Oenning et al, 6 the identification of cholesterol crystal clefts in the cytology is useful in determining a presumptive diagnosis of odontogenic cysts of inflammatory aetiology, and identifying parakeratin is suggestive of KCOT, which in the case of KCOT, eliminates the need for incisional biopsy to perform the planning of therapy for these lesions. None of the cases of ameloblastoma in the present study indicated the presence of cholesterol crystal clefts or keratin in the cytological analyses.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cell block technique as an additional tool in the diagnosis of ameloblastoma

et al. 2014

Self Cite

View full text Add to dashboard Cite

The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/ AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.

show abstract

Section: 67contrasting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cell block technique as an additional tool in the diagnosis of ameloblastoma

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is important to mention that some lesions present features that facilitate the cytological diagnosis. For example, the presence of parakeratin in both smear and cell block is proven to be very useful to distinguish odontogenic keratocysts from other jaw lesions with similar clinical and radiographic aspects . However, the analysis of FNAC diagnostic capability for individual odontogenic cysts was hindered due to limited sample size and absence of separated data in the included studies.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic capability of fine‐needle aspiration cytology in assessment of intraosseous lesions of the jaws: A systematic review

Pilati

Santos

Melo

et al. 2020

Diagnostic Cytopathology

View full text Add to dashboard Cite

Purpose To systematically review (SR) and critically appraise studies that investigated the diagnostic capability of fine‐needle aspiration cytology (FNAC) in the assessment of intraosseous lesions of the jaws. Methods Six main electronic databases and three gray literature databases were searched aiming diagnostic studies, which were selected in a two‐phase process. Risk of bias (RoB) of included studies was assessed using the Revised Tool for the Quality Assessment of Diagnostic Accuracy Studies. Results A total of eight articles were included, of which six evaluated the FNAC smear technique exclusively, one assessed both smear and cell‐block techniques, while one investigated only the cell‐block technique. With regard to the FNAC smear technique, from 134 benign lesions investigated, concordance with the histopathological diagnosis was achieved in 64.17% of cases. In addition, considering the 32 malignant lesions assessed through this technique, concordance with histopathology was achieved in all cases. Regarding the cell‐block technique, only benign lesions (n = 40) were evaluated in the included studies, in which the concordance rate with histopathology was of 77.5%. The overall RoB judgment was “at risk of bias” for seven included studies mostly due to lack of information or reportedly absence of blinding of evaluator. Conclusions Within the limitations of this SR, FNAC presented modest to satisfactory diagnostic capability for the assessment of intraosseous jaw lesions, showing concordance rates higher than 64% for the smear and 77% for the cell‐block technique. Also, considerably high concordance rates were found for the assessment of malignant lesions regarding FNAC smear technique.

show abstract

“…In the oral cavity, however, odontogenic pathologies, oral carcinomas, and other connective tissue lesions are more commonly diagnosed by the correlation between clinical, radiographic and tissue biopsy results. Although FNA cytology has been used in the diagnosis of odontogenic cysts, it mainly provides the diagnosis with the help of clinical and biochemical evaluation of the cyst fluid . FNA smears from odontogenic cysts and tumors provide very little information about the actual nature of the pathology .…”

Section: Introductionmentioning

confidence: 99%

Assessment of cell block technique in head and neck pathology diagnoses: A preliminary study

Desai

Angadi

Kale

et al. 2018

Diagnostic Cytopathology

View full text Add to dashboard Cite

Objective The head and neck region is a composite site made of multiple tissue components. These tissues when affected by disease or pathology present with an array of changes in the tissue architecture and pattern. It is essential to visualize the cellular details and tissue patterns for accurate diagnosis and treatment planning. Aspiration cytology primarily makes use of the cellular details for diagnosing lesions of the head and neck. Despite the promising results, its use is still limited in certain cases of the head and neck. The reason implicated could be the indiscernible appearance of cells in the absence of tissue integrity. In this regard, cell blocks are known to facilitate the visualization of the cytomorphological as well as the tissue arrangement patterns. Thus, the present study was designed to evaluate the role of cell block cytology in the diagnosis of various lesions of the head and neck. Methods Odontogenic lesions, epithelial carcinomas and connective tissue pathology of the head and neck origin were included in the study (n = 45). Aspiration cytology smears and cell block diagnosis were compared with tissue biopsy diagnosis for determining their sensitivity (%) and diagnostic efficacy. Results Cell blocks showed distinct preservation of the architectural pattern. In case of fluid‐filled lesions, the contents were preserved and correlated with the tissue biopsy results. The results of cell blocks were similar to that of tissue biopsy in majority of the cases (95.56%). Conclusion We recommend using cell blocks as a part of routine laboratory practice for all head‐neck cases.

show abstract

Utility of cell block in the cytological preoperative diagnosis of keratocystic odontogenic tumor

Cited by 10 publications

References 17 publications

Cell block technique as an additional tool in the diagnosis of ameloblastoma

Cell block technique as an additional tool in the diagnosis of ameloblastoma

Diagnostic capability of fine‐needle aspiration cytology in assessment of intraosseous lesions of the jaws: A systematic review

Assessment of cell block technique in head and neck pathology diagnoses: A preliminary study

Contact Info

Product

Resources

About