Utilization of arachidonic and linoleic acids by cultured human endothelial cells.

Spector, Arthur A.; Kaduce, Terry L.; Hoak, John C.; Fry, Glenna L.

doi:10.1172/jci110322

Cited by 170 publications

(46 citation statements)

References 29 publications

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“…Our finding is in agreement with the pattern found in cells and plasma of adults, i.e. that high linoleic acid is associated with low arachidonic acid (Spector et al, 1981;Liou and Innis, 2009;Friesen and Innis, 2010). The finding, if confirmed, might be of concern in view of the high intake of LA generally in food and the importance of arachidonic acid for the early development (Schuchardt et al, 2010).…”

supporting

confidence: 91%

Early behavior and development are influenced by the n-6 and n-3 status in prematures

Strandvik

Lundqvist-Persson

Sabel

2011

OCL

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supporting

confidence: 91%

Early behavior and development are influenced by the n-6 and n-3 status in prematures

Strandvik

Lundqvist-Persson

Sabel

2011

OCL

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“…Like human umbilical vein endothelial cells, 4 cultured bovine pulmonary artery endothelium has only a very limited capacity to convert linoleic acid to arachidonic acid. Less arachidonic acid remains in cellular lipids when the bovine endothelial cultures are supplemented with linoleic acid.…”

Section: Discussionmentioning

confidence: 99%

“…These cultures were found to convert only very small amounts of linoleic acid to arachidonic acid. 4 Furthermore, supplementation with linoleic acid produced a reduction in the arachidonic acid content of the cellular phospholipids and a decreased capacity of the cells to release PGI 2 .58 Similar effects of linoleic acid supplementation on prostaglandin production have not been observed in Madin-Darby canine kidney (MDCK) or 3T3 cells, both of which release predominantly prostaglandin E 2 (PGE 2 ). These cells accumulate increased amounts of arachidonic acid in phospholipids when they are enriched with linoleic acid, and PGE 2 production is enhanced.…”

mentioning

confidence: 96%

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Linoleic acid metabolism and prostaglandin production by cultured bovine pulmonary artery endothelial cells.

Kaduce¹,

Spector²,

Bar³

1982

Arteriosclerosis

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When bovine pulmonary artery endothelial cells are cultured in a medium supplemented with linoleic acid, their capacity to produce prostacyclln (PGI 2 ) is reduced by about 60%. This reduction occurs when PGI 2 formation Is stimulated by the addition of either the calcium lonophore A23187 or arachidonic acid. In addition, supplementation with linoleic acid reduced the production of prostaglandin E 2 and F 2n from 1-14 Carachidonic acid by more than 50%. The capacity of cultured bovine pulmonary vein and aortic endothelial cells to convert extracellular arachidonic acid Into PGI 2 also was reduced by about 50% when the growth medium was supplemented with linoleic acid. Although bovine pulmonary artery endothelial cells Incorporated large amounts of 1-14 C-linolelc acid into cellular phospholiplds and triglycerldes, a maximum of only 2.3% of the radioactivity was converted to arachidonic acid In 24 hours. The most prevalent radioactive metabolite was elcosadlenoic acid, the elongation product of linoleic acid. As compared with linoleic acid, the bovine endothelial cells Incorporated 30% more 1-14 C-arachldonlc acid Into phospholiplds and 60% more Into triglycerldes. When the growth medium was supplemented with linoleic acid, the percentage of this fatty acid In cellular liplds Increased 3-to 4.5-fold and eicosadlenoic acid accumulated, accounting for up to 9% of the cellular fatty acids. This Increase was accompanied by a 30% to 45% reduction in arachidonic acid. These findings, together with our previous results with human umbilical vein endothellum, suggest that an inability to convert large amounts of linoleic to arachidonic acid and a suppresslve effect of linoleic acid enrichment on prostaglandin production may be general properties of endothelial cells. 'The notation n-6 refers to the fact that the first double bond is 6 carbon atoms removed from the methyl terminus in this class of polyunsaturated fatty acids. Linoleic and arachidonic acids are members of the n-6 class.human umbilical vein endothelial cells revealed several unexpected results regarding linoleic acid metabolism and its effect on PGI 2 production. These cultures were found to convert only very small amounts of linoleic acid to arachidonic acid. 4 Furthermore, supplementation with linoleic acid produced a reduction in the arachidonic acid content of the cellular phospholipids and a decreased capacity of the cells to release PGI 2 .58 Similar effects of linoleic acid supplementation on prostaglandin production have not been observed in Madin-Darby canine kidney (MDCK) or 3T3 cells, both of which release predominantly prostaglandin E 2 (PGE 2 ). These cells accumulate increased amounts of arachidonic acid in phospholipids when they are enriched with linoleic acid, and PGE 2 production is enhanced.78 MDCK cells are derived from dog kidney tubular epithelium, and 3T3 cells are from a mouse embryo. Although these cells probably have different properties than cells of vascular origin, the fact that the findings were so different made it of interest ...

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“…or their viability [32]. To evaluate if the supplementary fatty acids can specifically regulate the level of mRNA in endothelial cells, we selected treatment conditions similar to those used for the metabolic studies [32,33]. Confluent third passage HUVEC were cultured in media with or without DGLA or DHA supplementation.…”

Section: Nucleotide Sequence Analysesmentioning

confidence: 99%

Stimulatory effect of unsaturated fatty acids on the level of plasminogen activator inhibitor‐1 mRNA in cultured human endothelial cells

et al. 1995

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To determine whether unsaturated fatty acids induce changes in the mRNA level of plasminogen activator inhibitor type-1 (PAI-I), Northern analyses were performed on human umbilical vein endothelial cells (HUVEC) and vascular smooth muscle cells that were treated with two common fatty acids. Supplementation of cultured HUVEC with docosahexanoic acid (DHA) or with dihomogamma linolenic acid (DGLA), resulted in a concentration dependent, specific increase of the PAI-1 transcript levels, which was detectable within 2 h. DHA and DGLA treatment of smooth muscle cells did not result in changes in the PAI-I mRNA levels. Homology search of the upstream regulatory region of the PAI-1 gene sequences identified a consensus nucleotide sequence for a fatty acid-responsive element. Our resuits indicate that unsaturated fatty acids selectively increase PAI-1 mRNA levels in endothelial cells, the primary source of circulating PAI-1 in vivo.

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Utilization of arachidonic and linoleic acids by cultured human endothelial cells.

Cited by 170 publications

References 29 publications

Early behavior and development are influenced by the n-6 and n-3 status in prematures

Early behavior and development are influenced by the n-6 and n-3 status in prematures

Linoleic acid metabolism and prostaglandin production by cultured bovine pulmonary artery endothelial cells.

Stimulatory effect of unsaturated fatty acids on the level of plasminogen activator inhibitor‐1 mRNA in cultured human endothelial cells

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