Stress factors, both biotic and abiotic, serve a significant role in impairing the growth and productivity of many crops. This study investigated the effects of some enzyme activities, salt scale, disease severity, plant growth, and physiological characteristics of arbuscular mycorrhizal (AM) fungi biochar (Bc) against Verticillium dahliae (Vd) in pepper plants grown under salt stress. In this study, 2% biochar, Funneliformis mosseae (Fm), Vd and different concentrations (0 mM, 50 mM, 100 mM, 150 mM) of salt were applied to pepper. It was determined that the interaction of Fm and 2% Bc significantly increased the morphological parameters of the plant, and the plant tolerated salt and Vd under biotic (Vd) and abiotic (salt) combined high stress conditions. It was also shown that Fm had a different effect on membrane injury index (MII) (%) values than Bc, which had a significant effect on leaf relative water content (LRWC) (%) values. In addition, Vd increased mycorrhizal dependence, and the highest AMF spore density under Vd and salt stress conditions was recorded in Fm and Fm+Bc+150 mM applications, respectively. Meanwhile, the highest root colonization rates were obtained in Fm and Fm + 100 mM applications at 92% and 64%, respectively. On the other hand, the lowest disease severity index values were found in Fm + Vd treatments with 11.06% and 9.68% rates, respectively. In addition, phenol and antioxidant values varied in applications with Vd, while Fm decreased CAT enzyme activation. This study, thus, supports the positive effect of AMF + biochar interaction used in sustainable agriculture on protection against soil pathogen (V. dahliae) and salt stress.