Utilization of Carotene from Oils

Rao, S. D.

doi:10.1038/156234c0

Cited by 5 publications

(1 citation statement)

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“…THE determination of carotene in plant tissues, the two general methods of removal of noncarotenoids are phasic and chromatographic separations (4,30,34)• In view of the recognized variations in the extent of digestion and absorption of carotene and its interdependence on the presence of varying amounts of antioxidants (1,7,14,15,16,39,40), complete chromatographic analysis, involving the separation of various carotene isomers, is neither practical nor necessary for routine analytical purposes, particularly in the nutritional evaluation of foods, feeds, and fodders (89). The phase separation method, on the other hand, is not always quantitative (11,83,24,25); certain biologically inert degradation products are also taken up in the epiphasic layer (36,50).…”

Section: Inmentioning

confidence: 99%

Determination of Carotene in Plant Materials

Sreenivasan¹,

Vaidya²

1948

Anal. Chem.

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A rapid method for the routine determination of carotene in plant materials has been worked out by which the green tissues are triturated with a mixture of lime and anhydrous sodium sulfate. This results in their disintegration as well as adsorption of chlorophyll and other noncarotenoid pigments; the carotene itself is rendered readily extractable by petroleum ether or benzene and can be determined either as gross carotene or as its isomerides after chromatographic resolution. The method is particularly suitable for the analysis of green, leafy materials, feeds, and fodders and gives excellent agreement with standard procedures, but it can be applied with equal success to fresh fruits and vegetables.

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