Utilization of Fermented Barley Extract Obtained from a By-product of Barley Shochu for Nisin Production

Furuta, Yoshifumi; Maruoka, Naruyuki; Nakamura, Akihiro; Omori, Toshiro; Sonomoto, Kenji

doi:10.1263/jbb.106.393

Cited by 23 publications

(14 citation statements)

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“…There is some controversy as to whether nisin should be considered a primary or a secondary metabolite [5,[30][31][32][33]. Difficulties in making direct correlations between cell growth rate and nisin production may stem from cryptic phenomena such as adsorption of the bacteriocins onto cell surfaces and/or the posttranslational processing of the prepeptides to active forms [9]. Additional mediaspecific variables (pH, nutrient availability, etc.)…”

Section: Resultsmentioning

confidence: 99%

“…[30] from cheese whey. Other researchers have reported 88.7 mg/L of nisin from cull potatoes [8], 1,233 IU/ml of nisin from fermented barley extract [9], and 16,000 IU/ml of nisin from hydrolyzed sago starch [41], to name a few. The ability to efficiently produce nisin using value-added agricultural waste materials such as soy whey may facilitate wider use of this once cost-restrictive antimicrobial.…”

Section: Discussionmentioning

confidence: 99%

“…Recent work has also focused on the use of nondairy substrates. For example, agricultural wastes such as cull potato hydrolysate and fermented barley extracts as well as fishery byproducts, including octopus and mussel processing wastes, have been examined as possible feedstocks for nisin fermentation [8][9][10][11]. In this study, we have evaluated soy whey, a byproduct stream from the soybean processing industry for nisin production as substrate for cultivating the strain L. lactis subsp.…”

Section: Introductionmentioning

confidence: 99%

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Value-Added Production of Nisin from Soy Whey

Mitra

Pometto

Khanal

et al. 2010

Appl Biochem Biotechnol

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The objective of this study was to evaluate the potential of low/negative value soy whey (SW) as an alternative, inexpensive fermentation substrate to culture Lactococcus lactis subsp. lactis for nisin production. Initially, a microtiter plate assay using a Bioscreen C Microbiology Plate Reader was used for rapid optimization of culture conditions. Various treatments were examined in efforts to optimize nisin production from SW, including different methods for SW sterilization, ultrasonication of soy flake slurries for possible nutrient release, comparison of diluted and undiluted SW, and supplementation of SW with nutrients. In subsequent flask-based experiments, dry bacterial mass and nisin yields obtained from SW were 2.18 g/L and 619 mg/L, respectively, as compared to 2.17 g/L and 672 mg/L from a complex medium, de Man-Rogosa-Sharpe broth. Ultrasonication of soybean flake slurries (10% solid content) in water prior to production of SW resulted in ∼2% increase in biomass yields and ∼1% decrease in nisin yields. Nutrient supplementation to SW resulted in ∼3% and ∼7% increase in cell and nisin yields, respectively. This proof-of-concept study demonstrates the potential for use of a low/negative value liquid waste stream from soybean processing for production of a high-value fermentation end product.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Value-Added Production of Nisin from Soy Whey

Mitra

Pometto

Khanal

et al. 2010

Appl Biochem Biotechnol

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“…FBE is a useful organic nutrient that can serve as a nitrogen and mineral source. 25) Hence, we also used FBE to simplify the medium …”

Section: Detection Of Biosurfactant Production By the Drop Collapse Mmentioning

confidence: 99%

Biosurfactant-producing yeasts widely inhabit various vegetables and fruits

Konishi

Maruoka

Furuta

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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The isolation of biosurfactant-producing yeasts from food materials was accomplished. By a combination of a new drop collapse method and thin-layer chromatography, 48 strains were selected as glycolipid biosurfactant producers from 347 strains, which were randomly isolated from various vegetables and fruits. Of the producers, 69% were obtained from vegetables of the Brassica family. Of the 48 producers, 15 strains gave relatively high yields of mannosylerythritol lipids (MELs), and were identified as Pseudozyma yeasts. These strains produced MELs from olive oil at yields ranging from 8.5 to 24.3 g/L. The best yield coefficient reached 0.49 g/g as to the carbon sources added. Accordingly, MEL producers were isolated at high efficiency from various vegetables and fruits, indicating that biosurfactant producers are widely present in foods. The present results should facilitate their application in the food and related industries.

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“…Ostlie et al (2005) evaluated the effect of temperature (20, 30, 37 and 45°C) on the growth and metabolism of six probiotic strains in an ultrahigh temperature (UHT) semi-skimmed milk medium supplemented with nutrients for 48 h. Their results showed that the stability of the number of viable cells was best at 30 and 37°C. Other researchers have focused on the use of industrial waste, such as milk whey (Arauz et al 2008), fermented barley extract (Furuta et al 2008) and musselprocessing wastes (Guerra et al 2005), as substrates to generate high-valued bioproducts and also to contribute to decreasing environment pollution.…”

Section: Introductionmentioning

confidence: 99%

Culture medium of diluted skimmed milk for the production of nisin in batch cultivations

et al. 2011

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Nisin is a promising alternative to chemical preservatives for use as a natural biopreservative in foods. This bacteriocin has also potential biomedical applications. Lactic acid bacteria are commonly cultivated in expensive standard complex media. We have evaluated the cell growth and nisin production of Lactococcus lactis in a low-cost natural medium consisting of diluted skimmed milk in a 2-L bioreactor. The assays were performed at 30°C for 56 h, at varying agitation speeds and airflow rates: (1) 200 rpm (no airflow, and airflow at 0.5, 1.0 and 2.0 L/min); (2) 100 rpm (no airflow, and airflow at 0.5 L/min). Nisin activity was evaluated using agar diffusion assays. The highest nisin concentration, 49.88 mg/L (3.3 log AU/mL or 1,995.29 AU/mL), was obtained at 16 h of culture, 200 rpm and no airflow (k L a=5.29×10 −3 ). These results show that a cultivation medium composed of diluted skimmed milk supports cell growth to facilitate nisin biosynthesis.

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Utilization of Fermented Barley Extract Obtained from a By-product of Barley Shochu for Nisin Production

Cited by 23 publications

References 23 publications

Value-Added Production of Nisin from Soy Whey

Value-Added Production of Nisin from Soy Whey

Biosurfactant-producing yeasts widely inhabit various vegetables and fruits

Culture medium of diluted skimmed milk for the production of nisin in batch cultivations

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