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Moury, Benoît; Palloix, Alain; Selassie, K. Gebre; Marchoux, Georges

doi:10.1023/a:1002997522379

Cited by 92 publications

(22 citation statements)

References 24 publications

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“…Although there are several reports on the ability of TSWV isolates to overcome resistance in capsicum lines following manual inoculation, the only reported instances of serious field infection of Tsw genotypes are from Italy (Roggero et al 2001(Roggero et al , 2002, Spain (Margaria et al 2004) and Australia (this work). Although Tsw-virulent isolates detected following serial manual inoculation of TSWV-resistant genotypes may compete effectively in field situations, it is also likely that some will be defective, having lost the envelope and associated glycoproteins during successive mechanical transfers, thus reducing or preventing transmission by thrips (Moury et al 1997;Sin et al 2005) All capsicum cultivars tested for resistance to TSWV were susceptible to the Tsw-virulent strain, indicating that a common source of resistance, the Tsw gene, was present in all and the cultivars' genetic background had little, if any, influence on the expression of the resistance gene.…”

Section: Discussionmentioning

confidence: 99%

“…Resistance expressed as a hypersensitive response and controlled by the single dominant gene Tsw has been found in the C. chinense accessions PI 152225, PI 159236 and Panca (syn. CNPH 275) (Black et al 1991;Moury et al 1997;Boiteux and de Avila 1994;Boiteux et al 1993;Boiteux 1995). Commercially available cultivars with this resistance are now grown in several countries including Australia (Roggero et al 2002;Persley et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Field isolates virulent towards the Tsw gene have been found in Louisiana (Hobbs et al 1994), Brazil (Boiteux and Nagata 1992), Italy (Roggero et al 2002) and Spain (Margaria et al 2004). Several workers have generated isolates able to overcome the Tsw gene and cause systemic infection following sap inoculation of resistant lines and serial passage of selected isolates (Moury et al 1997;Thomas-Carroll and Jones 2003).…”

Section: Introductionmentioning

confidence: 99%

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Field isolates ofTomato spotted wilt virusovercoming resistance in capsicum in Australia

Sharman

Persley

2006

Austral. Plant Pathol.

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Abstract. In 2002 at Virginia, South Australia, capsicum cultivars having the Tsw resistance gene against Tomato spotted wilt virus (TSWV) developed symptoms typical of TSWV infection and several glasshouse-grown crops were almost 100% infected. Samples reacted with TSWV antibodies in ELISA. Virus isolates from infected plants induced severe systemic symptoms, rather than a hypersensitive reaction, when inoculated onto capsicum cultivars and Capsicum chinense genotypes (PI 152225 and PI 159236) that carry the Tsw resistance gene. Isolates virulent towards the Tsw gene had molecular and biological properties very similar to standard TSWV isolates, including a hypersensitive reaction in Sw-5 (TSWV-resistant) tomato genotypes. Tsw-virulent isolates were found during surveys at Virginia in 2002 and 2004 in both TSWV-resistant and susceptible cultivars of capsicum and tomato.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Field isolates ofTomato spotted wilt virusovercoming resistance in capsicum in Australia

Sharman

Persley

2006

Austral. Plant Pathol.

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“…The resistance was expressed as a hypersensitive response and controlled by the dominant gene Tsw. (Black et al, 1991;Boiteux & De Avila, 1994;Moury et al, 1997). Unfortunately, the Tsw gene is not effective against other tospovirus species which are in the same Asian group as TNRV including Groundnut ringspot virus (GRSV), Tomato chlorotic spot virus (TCSV) (Boiteux & De Avila, 1994), Impatiens necrotic spot virus (INSV) (Roggero et al, 1999) and CaCV (McMichael et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Inheritance of Tomato Necrotic Ring Virus Resistance in Capsicum annuum

Puangmalai

Potapohn

Akarapisarn³

et al. 2013

JAS

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Tomato necrotic ring virus (TNRV) is a newly identified species of tospovirus in Thailand. The virus is widely spread and causes severe yield losses of vegetable crops such as tomato and pepper. Chemical control of the vector, thrips species, is very difficult, and the introduction of virus resistance would be a better approach to manage the disease.. In a previous study, two related Capsicum annuum lines, PY-6423 and PY-6424, were identified, which conferred medium resistance to TNRV. These two resistant lines were employed in an inheritance study in order to determine the number of genes controlling resistance in C. annuum. Therefore, the susceptible parent Early California wonder (ECW) was crossed with the resistant lines and F1 progenies were backcrossed to the susceptible and resistant parents as well as F2 were made. Evaluation of the F1, F2 and BC S and BC R indicated that the inheritance of the resistance fits a single recessive gene model.

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“…C. pubescens, C. baccatum, C. chinense and C. frutescens are good sources of characters for the breeding of C. annuum , in addition to their economic and agronomic relevance. For instance, the L gene involved in resistance to Tobacco mosaic virus [3] was introgressed into modern C. annuum cultivars from C. frutescens, C. chinense and the wild species Capsicum chacoense H., while the Tsw gene, which determines local lesion response to Tomato spotted wilt virus [4], was introgressed from C. chinense . Moreover, resistance to Cucumber mosaic virus , identified in C. baccatum , has been employed to develop several tolerant cultivars in France and Hungary [5].…”

Section: Introductionmentioning

confidence: 99%

EcoTILLING in Capsicum species: searching for new virus resistances

2010

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BackgroundThe EcoTILLING technique allows polymorphisms in target genes of natural populations to be quickly analysed or identified and facilitates the screening of genebank collections for desired traits. We have developed an EcoTILLING platform to exploit Capsicum genetic resources. A perfect example of the utility of this EcoTILLING platform is its application in searching for new virus-resistant alleles in Capsicum genus. Mutations in translation initiation factors (eIF4E, eIF(iso)4E, eIF4G and eIF(iso)4G) break the cycle of several RNA viruses without affecting the plant life cycle, which makes these genes potential targets to screen for resistant germplasm.ResultsWe developed and assayed a cDNA-based EcoTILLING platform with 233 cultivated accessions of the genus Capsicum. High variability in the coding sequences of the eIF4E and eIF(iso)4E genes was detected using the cDNA platform. After sequencing, 36 nucleotide changes were detected in the CDS of eIF4E and 26 in eIF(iso)4E. A total of 21 eIF4E haplotypes and 15 eIF(iso)4E haplotypes were identified. To evaluate the functional relevance of this variability, 31 possible eIF4E/eIF(iso)4E combinations were tested against Potato virus Y. The results showed that five new eIF4E variants (pvr210, pvr211, pvr212, pvr213 and pvr214) were related to PVY-resistance responses.ConclusionsEcoTILLING was optimised in different Capsicum species to detect allelic variants of target genes. This work is the first to use cDNA instead of genomic DNA in EcoTILLING. This approach avoids intronic sequence problems and reduces the number of reactions. A high level of polymorphism has been identified for initiation factors, showing the high genetic variability present in our collection and its potential use for other traits, such as genes related to biotic or abiotic stresses, quality or production. Moreover, the new eIF4E and eIF(iso)4E alleles are an excellent collection for searching for new resistance against other RNA viruses.

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Untitled

Cited by 92 publications

References 24 publications

Field isolates ofTomato spotted wilt virusovercoming resistance in capsicum in Australia

Field isolates ofTomato spotted wilt virusovercoming resistance in capsicum in Australia

Inheritance of Tomato Necrotic Ring Virus Resistance in Capsicum annuum

EcoTILLING in Capsicum species: searching for new virus resistances

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