Vaccination of lambs against Haemonchus contortus infection with a somatic protein (Hc23) from adult helminths

Fawzi, Elshaima M.; González‐Sánchez, Elena; Corral, María Jesús; Cuquerella, Montserrat; Alunda, José María

doi:10.1016/j.ijpara.2014.02.009

Cited by 31 publications

(31 citation statements)

References 26 publications

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“…86 In vaccine trials using two different adjuvants (aluminium hydroxide or E. coli lipopolysaccharide plus inactivated Propionibacterium acnes), lambs that received the Hc23 antigen in either adjuvant had significantly lower FECs and lower worm burdens after challenge compared to challenge control lambs. 86 Subsequently, the gene encoding the Hc23 antigen was identified, and a recombinant version expressed in E. coli. This protein was then used to vaccinate five-to six-month-old lambs three times in the context of aluminium hydroxide adjuvant.…”

Section: A Recombinant Vaccine For Haemonchosismentioning

confidence: 98%

“…Another protein (known as Hc23), this time identified in somatic extracts of adult H. contortus as the most abundant component of a low molecular weight fraction that had protective potential, was purified and tested in sheep . In vaccine trials using two different adjuvants (aluminium hydroxide or E. coli lipopolysaccharide plus inactivated Propionibacterium acnes ), lambs that received the Hc23 antigen in either adjuvant had significantly lower FECs and lower worm burdens after challenge compared to challenge control lambs . Subsequently, the gene encoding the Hc23 antigen was identified, and a recombinant version expressed in E. coli .…”

Section: Current Status Of Subunit Vaccines For the Main Gin Targets mentioning

confidence: 99%

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Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Matthews

Geldhof

Tzelos

et al. 2016

Parasite Immunology

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SummaryThe global increase in anthelmintic resistant nematodes of ruminants, together with consumer concerns about chemicals in food, necessitates the development of alternative methods of control for these pathogens. Subunit recombinant vaccines are ideally placed to fill this gap. Indeed, they are probably the only valid option for the long-term control of ruminant parasitic nematodes given the increasing ubiquity of multidrug resistance in a range of worm species across the world. The development of a subunit multicellular parasite vaccine to the point of practical application would be a groundbreaking step in the control of these important endemic infections of livestock. This review summarizes the current status of subunit vaccine development for a number of important gastrointestinal nematodes of cattle and sheep, with a focus on the limitations and problems encountered thus far, and suggestions as to how these hurdles might be overcome. K E Y W O R D Sgastrointestinal nematodes, ruminants, vaccines

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Section: A Recombinant Vaccine For Haemonchosismentioning

confidence: 98%

Section: Current Status Of Subunit Vaccines For the Main Gin Targets mentioning

confidence: 99%

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Matthews

Geldhof

Tzelos

et al. 2016

Parasite Immunology

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“…Infection with this parasite often results in anemia, diarrhea, weight loss, or even death in young animals, which causes large economic losses [3,4]. Mortality ranging from 30% to 50% has been reported in lambs and kids in acute cases [5]. Ruminants are infected by ingestion of the third-stage larva (L3).…”

Section: Introductionmentioning

confidence: 99%

Adhesion-Regulating Molecule from Haemonchus contortus: Potential Antigen for Diagnosis of Early Infection in Goats

et al. 2019

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Haemonchus contortus, a blood-sucking nematode of ruminants, causes large economic losses worldwide. Diagnosis of infection mainly depends on the evaluation of clinical signs and fecal examination. However, this has limitations for the diagnosis of early or light infections, where serological diagnosis seems to be more accurate and reliable. In this study, the recombinant H. contortus adhesion-regulating molecule protein (rHCADRM) was expressed and purified, and its diagnostic potential was evaluated. Serum samples from goats experimentally infected with H. contortus (n = 5) were collected at 0 (before infection, negative control), 7, 14, 21, 35, 49, 63, 85, and 103 days post-infection (DPI). The reactions between rHcADRM and goat serum were tested using Western blot (WB) analysis. The results show that rHcADRM can be recognized in the serum as early as 14 DPI, and the antibody against rHcADRM in infected goat could be maintained for over 89 days. No reaction was found between rHcADRM and antibodies against Trichinella spiralis, Fasciola hepatica, or Toxoplasma gondii. An indirect enzyme-linked immune sorbent assay (ELISA) was developed based on rHcADRM. The optimal coating antigen (279 ng of rHcADRM/well) and serum dilutions (1:50) were determined by checkerboard titration. A total of 64 serum samples, including 32 from H. contortus infection goats and 32 from helminth-free goats, were used to determine the positive (0.362) and negative (0.306) cut-off values for the ELISA. The results show this serological diagnosis method is highly sensitive (90.6%) and specific (93.75%). The coefficient of variation within run and between runs was less than 11%. To apply this indirect ELISA during field examination, 51 serum samples were randomly collected from goat farms and tested using this method. The result showed that 19.6% (10/51) of goats were infected with H. contortus, which was 100% consistent with the necropsy result, higher than that of fecal examination (15.7%, 8/51). These results indicate that rHcADRM could be a potential antigen for diagnosis of H. contortus infection in goats.

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“…The reduction after BW administration was lower than that observed for rPP2A-BW (59%), demonstrating the effectiveness of the stimulation of the response by the recombinant antigen. Also, when these BW were administered together with the recombinant antigen (rPP2A) the percentage of reduction in the number of worms would be comparable with levels found in other models of parasitic gastrointestinal nematodes of livestock when the same rPP2A-BW combination is used [59]. On the other hand, when rPP2A was linked with oleic-vinyl sulfone (rPP2A-OVS), the reduction rates of parasitism reached their highest levels, practically 98%.…”

Section: Discussionmentioning

confidence: 69%

Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced immunomodulation against Trichuris muris infection

et al. 2017

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Despite the importance of the adjuvant in the immunization process, very few adjuvants merge with the antigens in vaccines. A synthetic self-adjuvant oleic-vinyl sulfone (OVS) linked to the catalytic region of recombinant serine/threonine phosphatase 2A from the nematode Angiostrongylus costaricensis (rPP2A) was used for intranasal immunization in mice previously infected with Trichuris muris. The animal intranasal immunization with rPP2A-OVS showed a reduction of 99.01% in the number of the nematode eggs and 97.90% in adult. The immunohistochemical analysis of the intestinal sections showed that in immunized animals with lipopeptide the mucus was significantly higher than in the other experimental groups. Also, these animals presented significantly different chemokine, CCL20 and CCL11, levels. However, although the number and size of Tuft cells did not vary between groups, the intensity of fluorescence per cell was significant in the group immunized with the rPP2A-OVS. The results of the present study suggest that mice immunized with the lipopeptide are capable of activating a combined Th17/Th9 response. This strategy of immunization may be of great applicability not only in immunotherapy and immunoprophylaxis to control diseases caused by nematodes but also in pathologies necessitating action at the level of the Th9 response in the intestinal mucosa.

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Vaccination of lambs against Haemonchus contortus infection with a somatic protein (Hc23) from adult helminths

Cited by 31 publications

References 26 publications

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Adhesion-Regulating Molecule from Haemonchus contortus: Potential Antigen for Diagnosis of Early Infection in Goats

Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced immunomodulation against Trichuris muris infection

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