Vacuolar proteases and autophagy in phytopathogenic fungi: A review

Juárez-Montiel, Margarita; Clark-Flores, Daniel; Tesillo-Moreno, Pedro; Vega-Camarillo, Esaú de la; Andrade-Pavón, Dulce; Hernández-García, Juan Alfredo; Hernández‐Rodríguez, César; Villa-Tanaca, Lourdes

doi:10.3389/ffunb.2022.948477

Cited by 3 publications

References 82 publications

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Identification and Classification of Fungal GPCR Gene Families

Liu,

Salamov,

Grigoriev

2024

Preprint

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G protein-coupled receptors (GPCRs) are transmembrane proteins crucial for signal transduction in eukaryotes, responding to diverse extracellular signals. Researchers have found 14 distinct types of GPCRs in fungi, but their distribution among numerous fungal species has remained largely unexamined. Our study identified and classified GPCRs in 1,357 fungal species and shed light on their distribution in fungi. The predominant class detected in fungi was Pth11-like GPCRs, exclusively found in Pezizomycotina and notably recognized for their involvement in fungal pathogenicity. Our analysis suggested that the Pezizomycotina ancestor possessed a more extensive array of Pth11-like GPCRs, but over time, some species experienced considerable reductions in these GPCRs in conjunction with genome contractions. Additionally, we identified 2,089 mammalian homologs in the Rhodopsin, Glutamate, and Frizzled classes across 594 fungal species, thereby augmenting the recognized fungal GPCR classes by including three classes previously thought to be mammalian-specific. Utilizing a custom-built convolutional neural network (CNN) for the identification of fungal GPCRs, we discovered several potential novel fungal GPCRs. Moreover, predicted interactions between these prospective new GPCRs and G-alpha proteins, as simulated by AlphaFold Multimer, provided further validation for these findings.

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Identification and Classification of Fungal GPCR Gene Families

Liu,

Salamov,

Grigoriev

2024

Preprint

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Dynamic protein-protein interactions of the potato autophagy-related StATG8 family proteins, which are also associated with WRKY transcription factor and pathogen effector in the nucleus

Huh

2024

Preprint

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Background Autophagy is a cellular recycling process responsible for eliminating damaged proteins and organelles. Autophagy-related proteins (ATGs) are primarily located in the cytoplasm, playing essential roles in various stages of autophagosome formation. Among these proteins, the ATG8/Atg8/LC3/GABARAP family proteins are evolutionarily conserved and crucial for autophagy. While ATG8 is predominantly found in the nucleus and cytoplasm of cells, its function within the nucleus remains largely unknown. Results This study reveals that the potato StATG8 family forms homo-/hetero-dimers and selectively interacts with the transcriptional regulator WRKY, exclusive to the nucleus, and the pathogenic protein PopP2. Conclusions These findings represent a fundamental step toward unraveling the role of ATG8 within the nucleus.

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Plants and fungi metabolites as novel autophagy inducers and senescence inhibitors

Ofir

2024

Explor Drug Sci

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Premature aging can be partially explained by inefficient autophagy (the process of cellular self-digestion that recycles intracellular components) and premature senescence (cease of cellular division without cell death activation). Autophagy and senescence are among the basic biochemical pathways in plants and fungi suggesting that some of their metabolites have the potential to act as autophagy inducers (AI) and senescence inhibitors (SI) and to inhibit inflammation and human aging. Several compounds have already been identified: trehalose and resveratrol are natural compounds that act as AI; flavonoids found in fruit and vegetables (curcumin, quercetin, and fisetin) are among the first SI discovered so far. New AI/SI can be identified using various approaches like hypothesis-driven approach for screening receptor agonists using an in-silico library of thousands of natural compounds; cheminformatics studies of phytochemicals using docking and molecular dynamics simulation, structure similarities/mimicry in vitro, “blind” high throughput screening (HTS) of libraries of natural metabolites against relevant models, and more. This article aims to promote the use of plant and fungi novel resources to identify bioactive molecules relevant for healthy aging based on the knowledge that plants and fungi use autophagy and senescence mechanisms for their own survival and homeostasis. As autophagy and senescence are interconnected, how drugs targeting autophagy, senescence, or both could contribute to healthy aging in humans will be speculated.

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Vacuolar proteases and autophagy in phytopathogenic fungi: A review

Cited by 3 publications

References 82 publications

Identification and Classification of Fungal GPCR Gene Families

Identification and Classification of Fungal GPCR Gene Families

Dynamic protein-protein interactions of the potato autophagy-related StATG8 family proteins, which are also associated with WRKY transcription factor and pathogen effector in the nucleus

Plants and fungi metabolites as novel autophagy inducers and senescence inhibitors

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