Vaginoplasty Using Acellular Porcine Small Intestinal Submucosa Graft in Two Patients with Meyer-von-Rokitansky-Küster-Hauser Syndrome: A Prospective New Technique for Vaginal Reconstruction

Abstract

The objective of this case study is to present our experience of a surgical approach for vaginal agenesis using an acellular porcine small intestinal submucosa (SIS) graft. The present report involved 2 patients diagnosed as having vaginal agenesis due to Meyer-von-Rokitansky-Küster-Hauser syndrome. The operation procedure involved the creation of a neovaginal tunnel and then a mold wrapped with the SIS graft was placed in the neovagina. The duration of surgery was less than 45 min with minimal blood loss and no operative and postoperative complications. Epithelialization of the neovagina was achieved within 2 months after surgery. The neovagina created with this procedure was the same as that of a normal adult vagina histologically and physiologically. In conclusion, the creation of a neovagina using a SIS graft resulted in a favorable outcome and this approach may be a potential alternative to the management of vaginal agenesis.

Retrospective evaluation of corneal reconstruction usingACell Vet™alone in dogs and cats: 82 cases

“…Porcine small intestinal submucosa (SIS) and porcine urinary bladder matrix (UBM) have been used extensively to repair defects of the esophagus, bladder, cervicovagina, rectum, urethra, ureter, trachea, blood vessels, orbital floor, thoracic wall, and tendon and ligaments in humans and various animal species. SIS has been investigated extensively and has been used successfully in dogs, cats, and horses to repair various corneal or scleral defects .…”

Surgical Correction of Genitourinary Disorders Using the Self-Assembly Tissue Engineering

“…A wide variety of tissues and organs would benefit from engineering-based repair or regeneration. Several graft materials have been used to line the surgically created neovaginal cavity, including myocutaneous flaps or intestinal segments, full-thickness or split-thickness skin grafts, amniotic membrane, peritoneum, decellularized matrices, oral mucosa and vaginal epithelial tissues [32,[66][67][68][69][70][71][72][73]. These techniques are associated with graft contracture and/or stenosis that may require long-term dilatation.…”

Bioengineering Strategies to Treat Female Infertility

“…Vaginal agenesis, the congenital underdevelopment of the vagina, occurs in 1 out of 5000 females. [55][56][57] Various biomaterials have been used to fabricate vaginal grafts, including dermal matrix, 55,56,[58][59][60][61] SIS, 53,[62][63][64][65][66][67] and human amniotic membrane. 68 Recently, there have been several investigators that explored different biomaterials and cell sources to create tissue engineered constructs for vaginal agenesis therapies (Table 3).…”