Validated and rapid measurement of the ferric reducing antioxidant power in plasma samples

Gonzalez-Rivera, Maria L.; Martinez-Morales, Flavio; Alonso-Castro, Ángel Josabad; López-Rodríguez, Juan Francisco; Zapata-Morales, Juan Ramón; Aranda‐Romo, Saray; Aragón-Martínez, Othoniel Hugo

doi:10.1007/s11696-018-0512-9

Cited by 5 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assay was conducted as reported previously (Gonzalez‐Rivera et al, ) using a 96‐well plate. One hundred microliters of the test samples (DTA at 1,000–25,000 μg/mL or Trolox at 10–500 μg/mL) were combined with 250 μL sodium acetate (300 mM, pH 3.6), 25 μL of 10 mM 2,4,6‐tris(2‐pyridyl)‐s‐triazine prepared in 40 mM hydrochloric acid, and 25 μL of 20 mM iron (III) chloride hexahydrate.…”

Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory and diuretic effects of the diterpene ent‐dihydrotucumanoic acid

Alonso-Castro

Arana-Argáez

Devezé-Álvarez

et al. 2019

Drug Development Research

View full text Add to dashboard Cite

Gymnosperma glutinosum (Spreng) Less (Asteraceae) is a shrub used in traditional medicine for the treatment of inflammatory and renal diseases. The ent‐dihydrotucumanoic acid (DTA) is a diterpene obtained from G. glutinosum. This study evaluated the antioxidant, genotoxic, and diuretic properties of DTA, as well as its in vitro and in vivo anti‐inflammatory actions. The antioxidant actions of DTA were assessed with the 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulphonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and 2,2′‐diphenyl‐1‐picrylhydrazyl (DPPH) assays, the genotoxic action was assessed with the comet assay, and the diuretic effects of DTA were assessed using metabolic cages. The anti‐inflammatory actions were evaluated using primary murine peritoneal macrophages stimulated with LPS and the λ‐carrageenan‐induced hind paw edema test. DTA lacked antioxidant (IC50 > 25,000 μg/mL) activity in the ABTS, FRAP, and DPPH assays. DTA at 500–1,000 μg/mL showed moderate genotoxicity. In LPS‐stimulated macrophages, DTA showed IC50 values of 74.85 μg/mL (TNF‐α) and 58.12 μg/mL (NO), whereas the maximum inhibition of IL‐6 (24%) and IL‐1β (36%) was recorded at 200 μg/mL. DTA induced in vivo anti‐inflammatory effects with ED50 = 124.3 mg/kg. The in vitro anti‐inflammatory activity of DTA seems to be associated with the decrease in the release of TNF‐α and NO. DTA promoted the excretion of urine (ED50 = 86.9 mg/kg), Na+ (ED50 = 66.7 mg/kg), and K+ (ED50 = 8.6 mg/kg). The coadministration of DTA with L‐NAME decreased the urinary excretion shown by DTA alone. Therefore, the diuretic activity is probably associated with the participation of nitric oxide synthase. In conclusion, DTA exerted anti‐inflammatory and diuretic effects, but lacked antioxidant effects.

show abstract

Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory and diuretic effects of the diterpene ent‐dihydrotucumanoic acid

Alonso-Castro

Arana-Argáez

Devezé-Álvarez

et al. 2019

Drug Development Research

View full text Add to dashboard Cite

show abstract

“…The FRAP assay was performed as described previously. 21 In brief, one hundred microliters of saliva supernatant were added into a tube containing 250 µL of 300 mmol/L sodium acetate (pH 3.6), 25 µL of 10 mmol/L 2,4,6-tris(2-pyridyl)-s-triazine and 25 µL of 20 mmol/L iron (III) chloride hexahydrate. The mixture was incubated at 37°C for 4 minutes and then maintained at room temperature for 1 min.…”

Section: Salivary Antioxidant Capacity (Sac)mentioning

confidence: 99%

Correlation between salivary biomarkers and plaque-induced gingivitis exacerbated by pregnancy

Aragón-Martínez¹,

Loredo-Escobar²,

Bravo³

et al. 2022

J Oral Res

View full text Add to dashboard Cite

Objetive: To evaluate the correlation between salivary biomarkers (the salivary antioxidant ability, salivary level of polyphenols, and other antioxidants) with plaque-induced gingivitis exacerbated by pregnancy in pregnant and nonpregnant women. Material and Methods: For this observational study, medical records, dental examinations, and analyses of saliva samples were carried out in pregnant and nonpregnant women. A p-value of <0.05 was considered significant. Results: The pregnant women (n =17) exhibited a lower antioxidant capacity (p-value=0.0041), higher levels of polyphenols, gingival index, bleeding on probing, and subjects consuming mineral-enriched products (p-value from <0.0001 to 0.0466), and unchanged levels of phosphotungstic acid reactive substances, proteins, oral hygienic habits, plaque index and probing depth (p-value from 0.0683 to 0.8358), in comparison with the nonpregnant women (n=9). Also, a positive correlation between the gingival index and salivary polyphenol content was observed (r-value = 0.4087, p-value = 0.0202). Conclusion: The salivary polyphenols correlate with plaque-induced gingivitis exacerbated by pregnancy, suggesting a deficiency of salivary antioxidant protection.

show abstract

Matrix effect evaluation and validation of the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation scavenging assay, as well as its application using a tejate, an ancient beverage in Mexico

et al. 2019

View full text Add to dashboard Cite

Validated and rapid measurement of the ferric reducing antioxidant power in plasma samples

Cited by 5 publications

References 44 publications

Anti‐inflammatory and diuretic effects of the diterpene ent‐dihydrotucumanoic acid

Anti‐inflammatory and diuretic effects of the diterpene ent‐dihydrotucumanoic acid

Correlation between salivary biomarkers and plaque-induced gingivitis exacerbated by pregnancy

Matrix effect evaluation and validation of the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation scavenging assay, as well as its application using a tejate, an ancient beverage in Mexico

Contact Info

Product

Resources

About