Validated HPTLC method for quantification of vitamin D<sub>3</sub>in fish oil

Demchenko, D. V.; Pozharitskaya, Olga N.; Shikov, Alexander N.; Makarov, Valery G.

doi:10.1556/jpc.24.2011.6.6

Cited by 15 publications

(9 citation statements)

References 15 publications

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“…The solvent system chloroform/ diethyl ether (9:1, v/v.) was used as the mobile phase for the separation and quantification [13]. The component was visualized and quantified by direct densitometric scanning of the developed plate at 280 nm using a Camag TLC Scanner (Camag TLC scanner 3 under software control of WinCats v. 1.3.2, Muttenz, Switzerland).…”

Section: Cholecalciferolmentioning

confidence: 99%

Immunomodulatory and antioxidants properties of fixed combination of fish oil with plant extracts

Pozharitskaya¹,

Shikov²,

Косман³

et al. 2015

Synergy

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Section: Cholecalciferolmentioning

confidence: 99%

Immunomodulatory and antioxidants properties of fixed combination of fish oil with plant extracts

Pozharitskaya¹,

Shikov²,

Косман³

et al. 2015

Synergy

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“…After HPTLC separation, cholecalciferol (Vitamin D3) was detected at 280 nm. [10] Unsaturated lipids can be detected by UV densitometry at 190 nm, for example, from biodiesel and human plasma samples. [11][12][13][14] However, saturated lipids cannot be detected by UV.…”

Section: Direct Detecon and On-plate Stainingmentioning

confidence: 99%

“…[45] Vitamin D3 was also quantified in purified fish oil, and the on-silica UV spectrum from the sample was superimposed to this standard. [10] In all cases, appropriate standards were used for both characterization of migration distance (Rf) and for external calibration, using the Area of peaks.…”

Section: Semi-quantave and Quantave Analysesmentioning

confidence: 99%

“…External calibration for SQ analysis using densitometry has usually been performed using representative standards of the corresponding lipid sub-class. [10,19,27,28,[34][35][36][37]40,46,52,73,79,85,86,[95][96][97][98] A response factor system for quantitation was employed for a rapid and sensitive determination of MG, DG, TG and FFA in E471 emulsifiers by HPTLC with fluorescence densitometry using plates pre-chromatographically impregnated with primuline. [87] Calibration was done with 1,2-distearin and the amounts of lipid classes were calculated using response factors.…”

Section: Semi-quantave and Quantave Analysesmentioning

confidence: 99%

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Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Cebolla

Jarne

Vela

et al. 2021

Journal of Liquid Chromatography & Related Technologies

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This work is a review on HPTLC contribuon to lipid analysis in complex matrices, in the period from 2010 to now, lapse of me where hyphenaon with other techniques, especially Mass Spectrometry, has experienced an important growth.Scanning densitometry (SD)-UV/FL of separated lipids, as the core of detecon and centerpiece for hyphenaon, and its coupling with MS using so ionizaon techniques (ESI, APCI, MALDI, DESI and others), are the central axis of this work. The occasional intercalaon in this coupling of an on-plate biological assay for eect-direct analysis (EDA) of lipids, as well as the combinaon of SD with radiodensitometry (RD) using isotopically labeled-lipids are also covered topics.The described techniques make possible to develop strategies for obtaining qualitave and dierent levels of quantave informaon, including untargeted lipids species.HPTLC of lipids has been used for: comparave purposes; ngerprinng; semi-quantave determinaon, idencaon of species; or quantave determinaon of a given individual lipid. Goals in molecular biology and biochemistry-related samples have been: preparave isolaons; control of purity; vericaon of metabolic products; on-plate biological assays; proles in cells; analysis of products from the cellular metabolism; measurement of enzymac acvies; monitoring lipid transport across membranes or at biological interfaces; or monitoring lipid dynamics.

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“…These conflicting results reflect numerous confounding dietary elements. Indeed, fish and corn oil, which are generally used to investigate the effects of the ratio of n-3/n-6 PUFAs on disease development and progression, consist not only of n-3 and n-6 PUFAs, but also additional fatty acids and lipid soluble vitamins (25).…”

Section: Endogenously Synthesized N-3 Fatty Acids In Fat-1 Transgenicmentioning

confidence: 99%

Endogenously synthesized n-3 fatty acids in fat-1 transgenic mice prevent melanoma progression by increasing E-cadherin expression and inhibiting β-catenin signaling

Yin

Zhu

et al. 2016

Molecular Medicine Reports

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Malignant melanoma is the most lethal form of skin cancer. Although preclinical studies have shown that n-3 polyunsaturated fatty acids (PUFAs) are beneficial for prevention of melanoma, the molecular mechanisms underlying the protective effects of n‑3 PUFAs on melanoma remain largely unknown. In the present study, endogenously increased levels of n-3 PUFAs in the tumor tissues of omega‑3 fatty acid desaturase (fat‑1) transgenic mice was associated with a reduction in the growth rate of melanoma xenografts. This reduction in tumor growth in fat‑1 mice compared with wild‑type controls may have been associated, in part, to the: i) Increased expression of E‑cadherin and the reduced expression of its transcriptional repressors, the zinc finger E‑box binding homeobox 1 and snail family transcriptional repressor 1; ii) significant repression of the epidermal growth factor receptor/Akt/β‑catenin signaling pathway; and iii) formation of significant levels of n‑3 PUFA‑derived lipid mediators, particularly resolvin D2 and E1, maresin 1 and 15‑hydroxyeicosapentaenoic acid. In addition, vitamin E administration counteracted n‑3 PUFA‑induced lipid peroxidation and enhanced the antitumor effect of n‑3 PUFAs, which suggests that the protective role of n‑3 PUFAs against melanoma is not mediated by n‑3 PUFAs‑induced lipid peroxidation. These results highlight a potential role of n‑3 PUFAs supplementation for the chemoprevention of melanoma in high‑risk individuals, and as a putative adjuvant agent in the treatment of malignant melanoma.

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Validated HPTLC method for quantification of vitamin D₃in fish oil

Cited by 15 publications

References 15 publications

Immunomodulatory and antioxidants properties of fixed combination of fish oil with plant extracts

Immunomodulatory and antioxidants properties of fixed combination of fish oil with plant extracts

Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Endogenously synthesized n-3 fatty acids in fat-1 transgenic mice prevent melanoma progression by increasing E-cadherin expression and inhibiting β-catenin signaling

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Validated HPTLC method for quantification of vitamin D3in fish oil

Cited by 15 publications

References 15 publications

Immunomodulatory and antioxidants properties of fixed combination of fish oil with plant extracts

Immunomodulatory and antioxidants properties of fixed combination of fish oil with plant extracts

Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Endogenously synthesized n-3 fatty acids in fat-1 transgenic mice prevent melanoma progression by increasing E-cadherin expression and inhibiting β-catenin signaling

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Validated HPTLC method for quantification of vitamin D₃in fish oil