Validated LC-MS-MS Method for the Determination of Quetiapine in Human Plasma: Application to a Pharmacokinetic Study

Abstract

A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the determination of quetiapine was developed and validated over the linearity range 1-1500 ng/mL with 0.1 mL of plasma using clozapine as the internal standard. Detection was performed on a triple-quadrupole tandem mass spectrometer using positive electrospray ionization and quantification was performed by selected reaction monitoring mode. The MS-MS ion transitions monitored were m/z 384.1 → 253.1 and 327.0 → 270.0 for quetiapine and clozapine, respectively. The between- and within-run precision was less than 7.44% and accuracy was less than 10.2%. The lower limit of quantification was 1 ng/mL. The extraction recoveries of quetiapine were over 90%. The method is proved to be accurate and specific, and was applied to the pharmacokinetic study in healthy Chinese volunteers.

Rapid determination of quetiapine in blood by gas chromatography–mass spectrometry. Application to post‐mortem cases

“…() reported 0.00618 and 0.018 µg ml −1 using ultra performance liquid chromatography‐ tandem mass spectrometry (UPLC‐MS/MS) and Pan et al . () and Davis et al . () found LLOQ of 0.001 and 0.0005 µg ml −1 respectively, both using LC–MS/MS.…”

“…If we compare our analytical procedure with more sophisticated methodologies such as LC/tandem MS (LC-MS/MS), we find that they have lower LODs, as expected. Thus, Amundsen et al (2013) reported LOD and LLOQ of 0.00038 and 0.0038 μg ml À1 using an ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC)-MS/MS, Proença et al (2013) reported 0.00618 and 0.018 μg ml À1 using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and Pan et al (2012) and Davis et al (2010) found LLOQ of 0.001 and 0.0005 μg ml À1 respectively, both using LC-MS/MS. Despite these differences, our limit of quantification is low enough to detect the lower therapeutic concentrations.…”

Bioequivalence of two quetiapine extended release tablets in Chinese healthy volunteers under fasting and fed conditions and effects of food on pharmacokinetic profiles

“…As reported, there are many ways to quantitatively analyze quetiapine in human biological samples such as HPLC,28 HPLC-MS/MS,29,30 and liquid chromatography (LC)/MS/MS 19,24,31–33. However, method for BE study of 200 mg quetiapine XR in Chinese volunteers was only one; LC-MS/MS technology was used 34.…”

Development and validation of a high‐performance liquid chromatography–tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

“…Various analytical methods have been reported in the literature to detect each medication separately with their metabolites and/or in combination with other medications in rat and human plasma as well as urine samples. These include gas chromatography (GC; da Fonseca et al ., ), capillary electrophoresis (CE; Deng et al ., ), thin‐layer chromatography (TLC; Soliman and Youssef, ), high‐performance liquid chromatography (HPLC; Belal et al ., ; Yeniceli and Dogrukol‐Ak, ; Suryakant and Sadhana, ), liquid chromatography–mass spectrometry (LC/MS; Zhou et al ., ; Raman et al ., ; Choong et al ., ), gas chromatography–mass spectrometry (GC/MS; Kakde et al ., ), liquid chromatography–tandem mass spectrometry (LC/MS/MS; Moreira et al ., ; Suresh et al ., ; Fisher et al ., ; Pan et al ., ; Parekh et al ., ) and ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC/MS/MS) (Li et al ., ; Tu et al ., ; Denooz et al ., ). However, no method has been reported to simultaneously determine BUP, ESC and QUE in plasma.…”