2011
DOI: 10.1016/j.vetpar.2010.09.036
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Validation of a Leishmania infantum ELISA rapid test for serological diagnosis of Leishmania chagasi in dogs

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Cited by 38 publications
(36 citation statements)
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“…The need for a rapid serological test is evident from the numerous attempts to develop one in the past few decades (11,15,17,20,25,26,27,28,29). In comparison with other studies, in which immunochromatographic dipstick tests for L. infantum were compared to ELISAs and PCRs (16) or to DATs (17,29), and apart from the data of de Lima et al (30), who found a 91.5% sensitivity and a 94.7% specificity in an immunochromatography test based on the K39 antigen, the agreement between the immunochromatographic dipstick and other classical laboratory techniques (IFAT and WB) seems to be higher with the Speed Leish K, which performs similarly to a rapid ELISA-based test such as the Snap (11).…”
Section: Discussionmentioning
confidence: 99%
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“…The need for a rapid serological test is evident from the numerous attempts to develop one in the past few decades (11,15,17,20,25,26,27,28,29). In comparison with other studies, in which immunochromatographic dipstick tests for L. infantum were compared to ELISAs and PCRs (16) or to DATs (17,29), and apart from the data of de Lima et al (30), who found a 91.5% sensitivity and a 94.7% specificity in an immunochromatography test based on the K39 antigen, the agreement between the immunochromatographic dipstick and other classical laboratory techniques (IFAT and WB) seems to be higher with the Speed Leish K, which performs similarly to a rapid ELISA-based test such as the Snap (11).…”
Section: Discussionmentioning
confidence: 99%
“…Some rapid tests developed especially for dogs were shown to be highly sensitive and specific (11,15), while some immunochromatographic tests developed for and used in human medicine did not show high sensitivity or specificity when used for dogs (16,17). A rapid, sensitive, and specific diagnostic test might be relevant not only for mass-screening surveys but also for routine inclinic diagnosis, because the rapid and cost-effective detection of infected dogs is a key point in the control of infection and can greatly reduce the risk of infection transmission.…”
mentioning
confidence: 99%
“…In this case thorough diagnostics excluded potential alternative infections, such as E. canis which can cross react serologically (Ferreira et al, 2007 andMarcondes et al, 2011), primarily through the use of PCR analysis. Appropriate testing will depend upon geographic location and should be conducted in under the consultation or supervision of the relevant government authority as Leishmania is an OIE-listed disease (OIE, 2013).…”
Section: Discussionmentioning
confidence: 99%
“…Recently, Faria et al (62) performed an ELISA using a mixture of synthetic antigens and obtained high sensitivity and specificity values (78.5% and 80%, respectively); however, all of the synthetic antigens and the mixture presented high cross-reactivity with serum samples from T. cruzi-infected dogs. In addition, Marcondes et al (63), who performed a rapid ELISA, showed a high rate of false-positive results in which high percentages of cross-reactivity occurred between Leishmania spp. and T. cruzi (57%), as well as between Leishmania spp.…”
Section: Discussionmentioning
confidence: 99%