Validation of a Modified QuEChERS Method for the Determination of 167 Pesticides in Milk and Milk Products by LC-MS/MS

Gölge, Özgür; Koluman, Ahmet; Kabak, Bülent

doi:10.1007/s12161-017-1066-0

Cited by 44 publications

(7 citation statements)

References 23 publications

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“…In the case of cabbage and rice analyses using Thermo Scientific LC–MS/MS, an injection volume of 6 μL was selected to achieve optimum peak shape [ 47 ]. An injection volume of 20 μL was selected to provide the highest sensitivity in the case of milk and milk product analyses using Waters LC–MS/MS [ 48 ]. Therefore, it is necessary to optimize the injection volume.…”

Section: Resultsmentioning

confidence: 99%

Validation of a Multi-Residue Analysis Method for 287 Pesticides in Citrus Fruits Mandarin Orange and Grapefruit Using Liquid Chromatography–Tandem Mass Spectrometry

Yuan¹,

Kim²,

Lee³

et al. 2022

Foods

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Since the introduction of the positive list system (PLS) for agricultural products in the Republic of Korea, the demand for a quick, easy multi-residue analysis method increased continuously. Herein, the quick, easy, cheap, effective, rugged, and safe (QuEChERS) technique combined with liquid chromatography–tandem mass spectrometry was employed to optimize a method for the multi-residue analysis of 287 pesticide residues in mandarin orange and grapefruit. Method validation was conducted in terms of selectivity, limit of quantitation (LOQ), linearity, accuracy, precision, and matrix effect. All the compounds at low spiking levels (1, 2.5, 5, or 10 mg/kg) could be quantified at LOQs lower than 0.01 mg/kg (PLS level). The linearity of the matrix-matched calibration curve for each compound is in the range 0.5–50 μg/L, and its coefficient of determination (R2) is >0.990. Satisfactory recovery values of 70–120% with a relative standard deviation of ≤20% are obtained for all compounds in the mandarin orange and grapefruit samples. A negligible matrix effect (−20–20%) is observed for more than 94.8% and 85.4% of the pesticides in mandarin orange and grapefruit, respectively. Therefore, this analytical method can contribute to pesticide residue analyses of citrus fruits for routine laboratory testing.

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Section: Resultsmentioning

confidence: 99%

Validation of a Multi-Residue Analysis Method for 287 Pesticides in Citrus Fruits Mandarin Orange and Grapefruit Using Liquid Chromatography–Tandem Mass Spectrometry

Yuan¹,

Kim²,

Lee³

et al. 2022

Foods

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“…The recovery and precision were performed by the measurement of blank eggs matrices fortified at two concentration levels of 10 and 50 μg/kg. The expanded measurement uncertainty (U’) was measured by multiplying combined uncertainty associated with within-laboratory reproducibility and trueness (bias), with a coverage factor of k=2, as described in detail previously ( Golge et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Determination of more than 500 Pesticide Residues in Hen Eggs by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Gas Chromatography-Tandem Mass Spectrometry (GC/MS/MS)

Gölge¹,

Liman²,

Kabak³

2021

Food Sci Anim Resour

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This study aims to validate a fast method of simultaneous analysis of 365 LC-amenable and 142 GC-amenable pesticides in hen eggs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS), respectively, operating in multiple reaction monitoring (MRM) acquisition modes. The sample preparation was based on quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction. Key method performance parameters investigated were specificity, linearity, limit of quantification (LOQ), accuracy, precision and measurement uncertainty. The method was validated at two spiking levels (10 and 50 μg/kg), and good recoveries (70%–120%) and relative standard deviations (RSDs) (≤20) were achieved for 92.9% of LC-amenable and 86.6% of GC-amenable pesticide residues. The LOQs were ≤10 μg/kg for 94.2% of LC-amenable and 92.3% of GC-amenable pesticides. The validated method was further applied to 100 egg samples from caged hens, and none of the pesticides was quantified.

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“…The QuEChERS approach was rapidly and widely adopted by the scientific community due to the fact that it is a very flexible technique and its key parameters can be easily modified and optimized to increase its potential application in a wide range of different fields and matrices [1,2]. This technique has proven to be a reliable alternative for the extraction of pesticides from different matrices such as eggs [9,10], milk [11][12][13], honey [14,15], olives [16], fish muscle [17], soil [18][19][20][21], and water [20,22]. Based on its inherent advantages and great versatility, the QuECh-ERS was also used in analysis in different types of analytes including antibiotics [6,23,24], polycyclic aromatic hydrocarbons [25,26], and mycotoxins [27,28].…”

Section: Introductionmentioning

confidence: 99%

The pesticide residue analysis in commodities with high content of chlorophyll based on the quick, easy, cheap, effective, rugged, and safe method: A review

Pszczolińska

Kociołek

2021

J of Separation Science

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In multiresidue analysis, the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) is one of the most popular techniques routinely used by researchers during pesticide analysis of food and vegetable samples. Originally, the QuEChERS method was developed for analysis of pesticide residues from fruits and vegetables, but rapidly gained popularity in the extraction of analytes from different matrices. This analytical approach shows several advantages over traditional extraction techniques: it requires lower sample and solvent amounts while shortening the time of sample preparation. However, it presents some limitations for complex matrices such as those containing high amounts of chlorophyll. To overcome the problem of strong matrix effect and influence of interferences, different approaches are applied. Most are concerning modifications of the cleanup step, that is, sorbent type and its amount. Optimization of other parameters, such as sample size, hydration level, extraction solvent, and buffering, also has an impact on overall performance. Combining proper sample preparation with modern highly sensitive and selective detection techniques enables receiving desired limits of quantification. This article presents an overview of strategies employed by researchers for analysis of green, high chlorophyll content commodities and results obtained in their studies.

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Validation of a Modified QuEChERS Method for the Determination of 167 Pesticides in Milk and Milk Products by LC-MS/MS

Cited by 44 publications

References 23 publications

Validation of a Multi-Residue Analysis Method for 287 Pesticides in Citrus Fruits Mandarin Orange and Grapefruit Using Liquid Chromatography–Tandem Mass Spectrometry

Validation of a Multi-Residue Analysis Method for 287 Pesticides in Citrus Fruits Mandarin Orange and Grapefruit Using Liquid Chromatography–Tandem Mass Spectrometry

Determination of more than 500 Pesticide Residues in Hen Eggs by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Gas Chromatography-Tandem Mass Spectrometry (GC/MS/MS)

The pesticide residue analysis in commodities with high content of chlorophyll based on the quick, easy, cheap, effective, rugged, and safe method: A review

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